Izvo zvine maitiro akanaka uye anofambira mberi kune kufarira kwemutengi, sangano redu rinogara richinatsiridza zvigadzirwa zvedu kugutsa zvinodiwa nevatengi uye rinowedzera kutarisa nezve kuchengeteka, kuvimbika, mamiriro ezvakatipoteredza, uye kuvandudzwa kweFactory Supply China Yakanakira uye Kurumidza Rt-PCR Dspathtm 4X Imwe-Nhanho Multiplex Master Mix, Rutsigiro rwedu rwese runogona kunge rwuine ruzivo rwakaoma kushanda nesimba.Tinokugamuchira nemoyo wese kuti unyatsomira newebhusaiti yedu nekambani uye utitumire kubvunza kwako.
Izvo zvine maitiro akanaka uye anofambira mberi kune kufarira kwemutengi, sangano redu rinogara richivandudza zvigadzirwa zvedu kugutsa zvinodiwa nevatengi uye rinowedzera kutarisa nezve kuchengeteka, kuvimbika, zvakatipoteredza, uye hunyanzviChina Taq DNA Polymerase, Qpcr, Isu iye zvino tine mhinduro dzakanakisisa uye hunyanzvi hwekutengesa uye tekinoroji timu.Nekuvandudzwa kwekambani yedu, isu tinokwanisa kupa vatengi zvigadzirwa zvakanakisa, rubatsiro rwehunyanzvi rwehunyanzvi, basa rakakwana mushure mekutengesa.

Tsanangudzo

Iyi kit inoshandisa yakasarudzika lysis buffer sisitimu inogona kukurumidza kuburitsa RNA kubva kune yakasimwa maseru masampula eRT-qPCR maitiro, nekudaro ichibvisa iyo inopedza nguva uye yakaoma RNA yekuchenesa maitiro.Iyo RNA template inogona kuwanikwa mumaminetsi manomwe chete.Iyo 5 × Direct RT Musanganiswa uye 2 × Direct qPCR Musanganiswa-SYBR reagents anopihwa nekiti anogona nekukurumidza uye zvinobudirira kuwana chaiyo-nguva yehuwandu hwePCR mhinduro.

5 × Yakananga RT Mix uye 2 × Yakananga qPCR Mix-SYBR ine inhibitor kushivirira kwakasimba, uye lysate yemasampuli inogona kushandiswa se template yeRT-qPCR zvakananga.Iyi kit ine yakasarudzika RNA yakakwirira-yakabatana Foregene reverse transcriptase, uye Inopisa D-Taq DNA polymerase, dNTPs, MgCl.₂, reaction buffer, PCR optimizer uye stabilizer.

Zvinotsanangurwa

200×20μl Rxns, 1000×20μl Rxns

Kit zvikamu

Zvimiro&zvakanakira

■ Zviri nyore uye zvinoshanda : neTekinoroji yeCell Direct RT, masampuli eRNA anogona kuwanikwa mumaminetsi manomwe chete.

∎ Sample kudiwa idiki, yakaderera semaseru gumi anogona kuongororwa.

■ High throughput: inogona kukurumidza kuona RNA mumaseru akagadzirwa mu384, 96, 24, 12, 6-tsime mahwendefa.

∎ DNA Eraser inogona kukurumidza kubvisa majenomes akaburitswa, kuderedza zvakanyanya kukanganiswa pamhedzisiro yekuedza.

■ Optimized RT uye qPCR sisitimu inoita kuti nhanho mbiri RT-PCR reverse transcription inyatsoita uye PCR yakanyatsojeka, uye inoshingirira kuRT-qPCR reaction inhibitors.

Kit application

Kuwanda kwekushandiswa: masero akagadzirwa.

- RNA yakaburitswa nesample lysis: inoshanda chete kune RT-qPCR template yeiyi kit.

- Iyo kit inogona kushandiswa kune zvinotevera zvinangwa: gene kutaura kuongororwa, kuoneswa kweSiRNA-mediated gene kunyaradza maitiro, kuongorora zvinodhaka, nezvimwe.

Mufananidzo

Storage uye Sherufu upenyu

Chikamu I chekiti ichi chinofanira kuchengetwa pa4 ℃;Chikamu II chinofanira kuchengetwa pa -20 ℃.

Foregene Protease Plus II inofanira kuchengetwa pa 4 ℃, isaome pa -20 ℃.

Reagent 2 × Direct qPCR Mix-SYBR inofanira kuchengetwa pa -20 ℃ murima;kana ikashandiswa kakawanda, inogonawo kuchengetwa pa4 ℃ kuitira kuchengetedza kwenguva pfupi (shandisa mukati memazuva gumi) .Iyo ine mafungiro akanaka uye anofambira mberi kune kufarira kwemutengi, sangano redu rinogara richivandudza zvigadzirwa zvedu kuti rigutse zvinodiwa nevatengi uye rinowedzera kutarisa nezvekuchengeteka, kuvimbika, kutsanangurwa kwezvakatipoteredza, uye innovation yeFactory Supply Chinat-Rxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxt vashandi vane ruzivo rwakaoma vanogona kunge vachitsigira nemoyo wese.Tinokugamuchira nemoyo wese kuti unyatsomira newebhusaiti yedu nekambani uye utitumire kubvunza kwako.
Factory SupplyChina Taq DNA Polymerase, Qpcr, Isu iye zvino tine mhinduro dzakanakisisa uye hunyanzvi hwekutengesa uye tekinoroji timu.Nekuvandudzwa kwekambani yedu, isu tinokwanisa kupa vatengi zvigadzirwa zvakanakisa, rubatsiro rwehunyanzvi rwehunyanzvi, basa rakakwana mushure mekutengesa.

Real Time PCR primer dhizaini misimboti

Pamberi Primer uye Reverse Primer

Kune Real Time PCR, dhizaini yekutanga yakakosha.MaPrimers ane hukama nehunyanzvi uye kugona kwePCR amplification, uye inogona kugadzirwa ichitarisa kune anotevera misimboti:

Kureba kwekutanga: 18-30bp.

GC yemukati: 40-60%.

Tm kukosha: Primer dhizaini software, senge Primer 5, inogona kupa iyo Tm kukosha kweiyo primer.Iyo Tm tsika dzekumusoro uye pasi pemvura dzekutanga dzinofanira kunge dziri padyo sezvinobvira.The Tm calculation formula inogonawo kushandiswa: Tm = 4 °C (G + C) + 2 °C (A + T).Paunenge uchiita PCR, tembiricha iri pasi peiyo primer Tm kukosha kwe5 ° C inowanzo sarudzwa seye tembiricha yekudziya (iyo inowirirana kuwedzera kwekushisa kweanona inogona kuwedzera iyo chaiyo yePCR reaction).

Primers uye PCR zvigadzirwa:

Dhizaini primer PCR yekusimudzira chigadzirwa kureba ingangoita 100-150bp.

Dhizaini yekutanga munzvimbo yechipiri yemaitiro eiyo template inofanira kudziviswa zvakanyanya sezvinobvira.

Dzivisa kuumbwa kwe 2 kana anopfuura mabhesi anowirirana pakati pe 3′ migumo yekumusoro nekudzika kwemvura.

Primer 3′ terminal base haigone kuvepo ne3 yekuwedzera yakatevedzana G kana C.

Iwo maprimer pachawo haagone kuve neanopindirana zvimiro, zvikasadaro chimiro chebvudzi chinoumbwa, chinokanganisa PCR amplification.

ATCG inofanirwa kugovaniswa zvakaenzana sezvinobvira mukutevedzana kwekutanga, uye 3 ′ terminal base inofanira kudzivirirwa seT.

Appendikisi 1: Cell Direct RT-qPCR Kit chikamu chekuwedzera paki

1.Sero Lysis Solution