Isu tinotevera manejimendi ehutongi hwekuti "Hunhu hwakasarudzika, Rubatsiro rwakanyanya, mukurumbira ndewekutanga", uye tichagadzira nemoyo wese kubudirira uye kugovana budiriro nevatengi vese veChina wholesale China Magpure Ffpe Rna Kit yeNucleic Acid Isolation& Yekuchenesa Kit, Isu tinovimbisa kuyedza zvatinokwanisa kuti tikupe iwe nemhando yepamusoro uye zvigadzirwa zvinoshanda nemasevhisi.
Isu tinotevera manejimendi ehutongi hwekuti "Hunhu hwakasarudzika, Rubatsiro rwakanyanya, mukurumbira ndewekutanga", uye tichagadzira nemoyo wese kubudirira uye kugovana budiriro nevatengi vese.China Rna / DNA Extraction Kucheneswa, Nucleic Acid Isolation, Nehurongwa hwekushanda hwakanyatsobatanidzwa, kambani yedu yakawana mukurumbira wakanaka wezvigadzirwa zvedu zvemhando yepamusoro, mitengo inonzwisisika uye masevhisi akanaka.Zvichakadaro, ikozvino takagadzira yakasimba yemhando yekutonga system inoitiswa mune zvinhu zvinouya, kugadzirisa uye kuendesa.Tichitevedzera musimboti we "Chikwereti chekutanga uye hukuru hwevatengi", tinotambira nemoyo wese vatengi vanobva kumusha nekune dzimwe nyika kuti tibatane nesu uye tifambire mberi pamwechete kugadzira ramangwana rakajeka.

Zvinotsanangurwa

50 Preps, 200 Preps Kit inoshandisa spin column uye fomula yakagadziridzwa neForegene, iyo inogona kunyatso tora yakakwirira-kuchena uye yepamusoro-soro yakazara RNA kubva kwakasiyana siyana matishu ane yakakwira polysaccharides kana polyphenols zvirimo.Inopa iyo DNA-Kuchenesa koramu iyo inogona nyore kubvisa genomic DNA kubva kune supernatant uye tishu lysate.RNA-chete column inogona kunyatsosunga RNA.Iyo kit inogona kugadzirisa nhamba huru yemasampuli panguva imwe chete.

Iyo yese system haina RNase, saka iyo yakacheneswa RNA haizodzikisirwe.Buffer PRW1 uye Buffer PRW2 inogona kuvimbisa kuti RNA inowanikwa haina kusvibiswa neprotein, DNA, ions, uye organic compounds.

Kit zvikamu

Zvimiro&zvakanakira

■ Kushanda pakamuri tembiricha (15-25 ℃) mukati mese muitiro, pasina kugeza nechando uye yakaderera tembiricha centrifugation.■ Yakazara kit RNase-Yemahara, hapana chikonzero chekunetseka nezveRNA kudzikira.∎ Inonyanya kukodzera kucheneswa kweRNA kubva kumiti yemichero yemapolysaccharides uye polyphenols.∎ DNA-Cleaning Column inosungirira kuDNA, kuitira kuti kit ibvise kusvibiswa kweDNA yegenomic pasina kuwedzera DNase.■ Yakakwira RNA goho: RNA-chete Column uye yakasarudzika fomula inogona kunyatsochenesa RNA.■ Kukurumidza kumhanya: nyore kushanda uye inogona kupedzwa mukati memaminitsi makumi matatu.■ Kuchengetedza: hapana organic reagent inodiwa.■ Hunhu hwepamusoro: Zvimedu zveRNA zvakacheneswa ndezvekuchena kwepamusoro, hazvina mapuroteni nehumwe tsvina, uye zvinokwanisa kusangana nemhando dzakasiyana dzekuedza dzepasi.

Product parameters

■ Zvishandiso zvepasi: yekutanga-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, zvichingodaro. ■ Muenzaniso: Zvitsva kana zvakaomeswa zvechirimwa tishu zvepolysaccharides nemapolyphenols ■ Muyero: 50mg chirimwa tishu ■ Yakanyanya RNA inosunga simba rekuchenesa koramu: 80 μg vhoriyamu: ■ 50 Elution2 ■ 50 Elution

Kit application

Yakakodzera kubviswa uye kucheneswa kweRNA yakazara kubva kutsva kana chando matishu echirimwa samples (kunyanya nyowani yemashizha emuti wetishu) ine yakakwira polysaccharide uye polyphenol zvemukati.

Kuyerera kwebasa

Mufananidzo

Plant Yese RNA Isolation Kit Plus yakagadziriswa 50mg yemashizha matsva epolysaccharides uye polyphenols, uye 5% yakacheneswa RNA yakaedzwa ne electrophoresis.1: Banana 2: Ginkgo 3: Donje 4: Damba

Storage uye Sherufu upenyu

Iyi kit inogona kuchengetwa kwemwedzi makumi maviri nemana pasi pemamiriro akaoma pane tembiricha yekamuri (15-25 ℃);kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa mu2–8℃.Buffer PSL1 inogona kuiswa pa 4 ℃ kwe 1 mwedzi mushure mekuwedzera β-mercaptoethanol (inokurudzirwa kuiwedzera panguva imwe chete yekuedza) .Tinotevedza dzidziso yehutungamiri ye "Unhu hunoshamisa, Rubatsiro rwakanyanya, Reputation ndiyo yekutanga", uye tichagadzira nemwoyo wose kubudirira nevatengi vose veChina yeFlasalef China Magpuret.Nucleic Acid Isolation& Yekuchenesa Kit, Isu tinovimbisa kuyedza zvatinokwanisa kuti tikupe iwe nemhando yepamusoro uye zvigadzirwa zvinoshanda nemasevhisi.
China wholesaleChina Rna / DNA Extraction Kucheneswa, Nucleic Acid Isolation, Iine yakanyatsobatanidzwa oparesheni system, kambani yedu yahwina mukurumbira wakanaka wemhando yepamusoro yekutengesa, mitengo inonzwisisika uye masevhisi akanaka.Zvichakadaro, ikozvino takagadzira yakasimba yemhando yekutonga system inoitiswa mune zvinhu zvinouya, kugadzirisa uye kuendesa.Tichitevedzera musimboti we "Chikwereti chekutanga uye hukuru hwevatengi", tinotambira nemoyo wese vatengi vanobva kumusha nekune dzimwe nyika kuti tibatane nesu uye tifambire mberi pamwechete kugadzira ramangwana rakajeka.

Iyo column yakavharwa

Mushure mekunge koramu yavharwa, goho reRNA rinodzikiswa kana kutotadza kuchenesa iyo RNA, uye iyo yakawanikwa RNA misa yakaderera.

Kazhinji chikonzero chekuongorora:

1. Sample breaks haisi yakakwana.

Sample kuputsika hakukonzerese zvachose kuti DNA-CLEANING COLUMN ivharwe, uku ichikanganisa goho reRNA uye mhando.Isu tinokurudzira kukurumidza kukuya kushanda mune yakakwana mvura nitrogen pawakatyora masampuli, Edza kupwanya sampuli sero madziro, sero membrane uye mamwe matishu.Kune zvidyarwa zvemasampunzi e polyol polysaccharides, isu tinokurudzira kuti iwe ushandise Plant Yese RNA IsolATION KIT PLUS.

2. Kana ukasveta sample yakapatsanurwa supernatant neDNA-Cleaning Column, sero rinobvira rakatsemuka mvura inogona kufemerwa.

Iyo sero yakatsemuka sediments yakatorwa inokonzeresa iyo RNA-CHETE Column iyo ichavharika kana RNA adsorption oparesheni yaitwa (ona danho rechitanhatu).Isu tinokukurudzira nekuchenjerera paunosveta iyi supernatant kudzivirira maseru mamarara ari kuyamwa.

3. Sample yekutanga mari yakawandisa.

Kunyanya kushandiswa kwemuenzaniso kuchaita kuti isapera kupatsanurwa kwemuenzaniso kana kusakwana sero lysis neBuffer PSL1, zvichikonzera kuvharika kwekoramu yekuchenesa panguva yekucheneswa.Plant Yese RNA Isolation Kit Imwe neimwe yakanatswa inoshanda sample i50 mg.Kune zvidyarwa zvemasampunzi e polyol polysaccharides, isu tinokurudzira kuti uedze Kudyara Yese RNA IsolATION KIT PLUS.

4. Kupisa kwe centrifuge kwakanyanya kuderera.

Iyo yese RNA yekuzviparadzanisa uye yekucheneswa maitiro inoitwa pane yekudziya tembiricha (20-25°C), kunze kwekunge iyo sampuli tishu yakatyoka ne nitrogen yemvura. Tembiricha yemamwe cryogenic centrifuges yakaderera kupfuura 20.℃, izvo zvinogona kukonzera kuvharika kweDNA-Cleaning Column uye/kana RNA-Chete Column.Kana izvi zvikaitika, isa tembiricha ye centrifuge kusvika 20-25℃,uyeita shuwa kuti musanganiswa welysis uye/kana iyo ethanol-yakawedzerwa supernatant yakapiswa kusvika 37°C.

Hapana RNA yakabviswa kana RNA goho rakaderera

Pane kazhinji zvinhu zvakawanda zvinokanganisa kugona kudzoreredza, senge: sampuli RNA yemukati, nzira yekushanda, iyo elution vhoriyamu, nezvimwe.

Kuongororwa kwezvikonzero zvakajairika sezvinotevera:

1.Kushamba kwechando kana kutonhora kwakadzika (4 ° C) centrifugation yakaitwa panguva yekushanda.

Zano: Shanda patembiricha yekamuri (15-25°C) mukuita kwese, usaite ice bath uye yakaderera tembiricha centrifugation.

2.RNA yakasvibiswa nekuda kwekuchengetedzwa kusina kunaka kwemuenzaniso kana kuchengetedza kwenguva refu kwemuenzaniso.

Kurudziro: Zvichangobva kuunganidzwa samples dzinofanirwa kukurumidza kuomeswa nechando mumvura ine nitrogen, yozochengetwa pa -80°C kwenguva yakareba, kudzivisa kuomesa nechando uye kunyungudutswa kwemasampuli;kana kuti nekukasira kunyudza masampuli muRNA stabilizer RNAlater mhinduro (samples dzemhuka).

3.Kusakwana kupatsanurwa kwemuenzaniso uye lysis kunotungamirira kukuvhara kwekoromo yekuchenesa.

Mazano: Paunenge uchikuya mutsara, tapota chengetedza kuti mutsara wakakwana pasi, uye nekukurumidza uende nayo kuBuffer pre-prepared PSL1 (simbisa kuti chikamu chakarurama che β-ME chakawedzerwa, ona danho rekutanga rekuita).

4.The eluent yakawedzerwa zvisizvo.

Kurudziro: Ita shuwa kuti RNase-Yemahara ddH2O yakadonhedzwa pakati pechenese column membrane.

5.Iyo chaiyo vhoriyamu yeabsolute ethanol haina kuwedzerwa kuBuffer PSL2 kana Buffer PRW2.

Zvaungaita: Tapota tevera mirairo, wedzera huwandu chaihwo hweabsolute ethanol kuBuffer PSL2 neBuffer PRW2 uye sanganisa zvakanaka kititi isati yashandiswa.

6.Kuwanda kwemuenzaniso wenyama hakuna kufanira.

Zano: Shandisa 50 mg yetishu pa500 μl yeBuffer PSL1.Kushandisa nyama yakawandisa kunoderedza huwandu hweRNA yakabviswa uye kuchena kweiyo RNA inoguma kunodzikiswa zvakare.Isu tinokurudzira zvakasimba kuti yekutanga sampuli dosage haifanire kudarika 50 mg paRNA yekubvisa oparesheni.

7.Inappropriate elution volume kana kusakwana.

Zano: Vhoriyamu yakasarudzika yekoramu yekuchenesa ndeye 50-200 μl;kana iyo elution mhedzisiro isiri inogutsa, zvinokurudzirwa kuwedzera nguva pakamuri tembiricha mushure mekuwedzera preheated RNase-Mahara ddH2O, senge 5-10min.

8.Koramu yekuchenesa ine ethanol yakasara mushure mekugeza neBufferPRW2.

Zano: Kana iyo chubhu isina chinhu iine centrifuged ye1 min uye ichine ethanol yakasara mushure mekugezwa muBuffer PRW2, unogona kuwedzera nguva yechubhu isina chinhu centrifugation kusvika ku2 min, kana kuisa mbiru yekuchenesa pakupisa kwekamuri ye5 min kuti ubvise zvachose ethanol yakasara.

9.Kiti yakashandiswa zvisizvo.

Zano: Kune zvidyarwa zvepolyphenolic polysaccharides, uchishandisa zvakajairika kits sePlant Yese RNA Isolation Kit inogona kusakwanisa kuwana yakakodzera RNA samples.Isu tinokukurudzira kuti ushandise Plant Yese RNA IsolationKit Plus, iyo yakanyatsogadzirirwa polyphenolic polysaccharide chirimwa samples.Kiti yakagadziridzwa kuburitsa RNA kubva ku polyphenol uye polysaccharide chirimwa samples.

OD260/OD280 kukosha kwakaderera

RNA elution ine ddH2O uye inoshandiswa kuverenga spectrophotometer inoguma yakaderera OD260/OD280 kukosha.Tinokurudzira kushandisa 10 mM Tris-HCl, pH 7.5 (panzvimbo peRNase-Yemahara ddH2O kuti utore RNA) kuti uwane zvakati tsvikiti zveOD260/OD280, ona “RNA Concentration and Purification Assays” papeji 19.

Iyo RNA yakacheneswa yakaderedzwa

Hunhu hweRNA yakacheneswa hune hukama nezvinhu zvakaita sekuchengetedza sampuli, kusvibiswa kweRNase, uye kunyengera.

Kuongororwa kwezvikonzero zvinowanzoitika:

1.Tissue samples haana kuchengetwa munguva mushure mekuunganidza.

Kurudziro: Kana masampuli ematishu asina kushandiswa munguva mushure mekuunganidzwa, ndapota achengetedze mu nitrogen yemvura pa tembiricha yakaderera pakarepo kana kuti uendese kune -80 ° C kuitira kuchengetedza kwenguva refu mushure mekukurumidza kutonhora mu nitrogen yemvura, kana kunyudza nekukurumidza masampuli muRNA stabilizer RNAlater solution (mhuka samples ).Nekubvisa RNA, edza kushandisa masampula ematishu achangobva kuunganidzwa.

2.Kudzokororwa kwechando uye kunyunguduka kwemasampuli ematishu.

Zvaungaita: Paunenge uchichengeta matishu esampuli, zviri nani kuacheka kuita zvidimbu zvidiki kuti zvichengeteke, uye wotora chikamu chawo paunenge uchiashandisa kudzivirira kuparara kweRNA kunokonzerwa nekuomesa nechando kakawanda uye kunyunguduka kwemasamples acho.

3.RNase inounzwa mukamuri yekuvhiya kana kusapfekwa magirovhosi anoraswa, masiki, nezvimwe.

Zano: RNA kutorwa kwekuyedza kunoitwa zvakanyanya mumabasa akasiyana eRNA, uye tafura yemurabhoritari inofanirwa kucheneswa kusati kwaedzwa, uye magirovhosi nemasks anoraswa anofanira kupfekwa panguva yekuyedza kudzivirira kuparara kweRNA kunokonzerwa nekuunzwa kweRNase zvakanyanya.

4.Iyo reagent yakasvibiswa neRNase panguva yekushandiswa.

Zano: Tsiva nenhevedzano itsva yechirimwa yakazara RNA yekubvisa kits yezviyedzo zvinoenderana.

5.The centrifuge tubes uye pipette mazano anoshandiswa paRNA manipulation akasvibiswa neRNase.

Zvaungaita: Ita shuwa kuti machubhu ecentrifuge, matipi epipette, mapipetti, nezvimwewo zvinoshandiswa muRNA kudhirowa zvese zviri RNase-Mahara.