Kutengesa Kutengesa kweImwe-Nhanho Probe Rt-Qpcr Kit Yekutsvaga Shandisa Chete V5001 200 Rxn
Kit Description:
◮Nyore-2 × PCR Sanganisa kuderedza kuyedza kukanganisa uye nguva yekushandisa
◮Yakananga-yakagadziridzwa buffer uye inopisa-kutanga Taq enzyme inogona kudzivirira isina-chaiyo amplification uye primer dimer kuumbwa.
◮Kunzwa kwakanyanya-inogona kuona yakaderera makopi etemplate
◮Zvakanaka kuita zvakasiyana-siyana-inoenderana neyakawanda-chaiyo-nguva yakawanda PCR zviridzwa
Komisheni yedu inofanirwa kunge iri yekushandira vashandisi vedu vekupedzisira nevatengi nemhando yepamusoro yepamusoro uye inokwikwidza inotakurika zvigadzirwa zvedhijitari uye mhinduro dzeKutengesa Kutengesa kune Imwe-Nhanho Probe Rt-Qpcr Kit Yekutsvagisa Shandisa V5001 200 Rxn Chete, Nheyo yebhizinesi redu inowanzo kupa zvinhu zvemhando yepamusoro, masevhisi ane hunyanzvi, uye kutaura kwechokwadi.Gamuchirai vese vakaroorana kuti vaise odha yekuedza yekugadzira hukama hwesangano kwenguva refu.
Komisheni yedu inofanirwa kunge iri yekushandira vashandisi vedu vekupedzisira nevatengi vane yakanakisa mhando yepamusoro uye inokwikwidza inotakurika zvigadzirwa zvedhijitari uye mhinduro dze.China Taq DNA Polymerase uye Qpcr, Zvinhu zvedu zvinotengeswa pasi rose.Vatengi vedu vanogara vachigutsikana nemhando yedu yakavimbika, masevhisi anotungamirwa nevatengi uye mitengo yemakwikwi.Basa redu ndere "kuramba tichiwana kuvimbika kwako nekuzvipira kuedza kwedu mukuvandudza nguva dzose kwezvinhu zvedu nemasevhisi kuitira kuti tive nechokwadi chekugutsikana kwevashandisi vedu vekupedzisira, vatengi, vashandi, vatengesi uye nharaunda dzepasirese dzatinoshandira pamwe".
Kit Descriptions
Iyo 2X Chaiyo PCR iri NyoreTMMix-Taqman yakapihwa neiyo Real Time PCR EasyTM-Taqman kit inyowani premix sisitimu inoshandisa chaiyo fluorescent probes yeReal Time PCR amplification reactions, inogona kuvandudza zvakanyanya chigadzirwa chakanangana nekuita senitivity.ROX inopihwa seyemukati yekudzora dhayi.
2X Real PCR NyoreTMMix-Taqman ine Forgene yakasarudzika inopisa-kutanga Taq DNA Polymerase.Kuenzaniswa neakajairwa maTaq enzymes, ine mabhenefiti ekukwirira kwepamusoro, kugona kwakasimba kwekusimudzira uye yakaderera mismatch mwero.Inogona kuderedza kusiri-chaiyo amplification uye kunatsiridza huchokwadi hwePCR.
Zvinotsanangurwa
| Real Time PCR EasyTM-Taqman | ||||
| Kit kuumbwa (20μl system) | QP-01021 | QP-01022 | QP-01023 | QP-01024 |
| 200T | 500T | 1000T | 2000T | |
| 2 × Real PCR EasyTMMix-Taqman | 1 ml × 2 | 1.7 ml × 3 | 1.7 ml × 6 | 1.7 ml × 12 |
| 20 × ROX Reference Dye | 200 μl | 0.5 ml | 1 ml | 1 ml × 2 |
| DNase-Yemahara ddH2O | 1.7 ml | 1.7 ml × 2 | 10 ml | 20 ml |
| Instruction | 1 | 1 | 1 | 1 |
Zvimiro&zvakanakira
■ Nyore—2X PCR Sanganisa kuderedza chikanganiso chekuedza uye nguva yekushandisa
■ Yakananga-yakagadziridzwa buffer uye inopisa-kutanga Taq enzyme inogona kudzivirira isiri-chaiyo amplification uye primer dimer kugadzirwa.
■ Kunzwa kwakanyanya-inokwanisa kuona makopi akaderera etemplate
■ Kugona kuita zvakasiyana-siyana-kunoenderana nezvizhinji-chaiyo-chaiyo-chaiyo-chaiyo-yehuwandu PCR zviridzwa
Kit application
qPCR ongororo
Kuyerera kwebasa
Graphic
Kuchengetedza uye hupenyu hwesherufu
Iyi kit inofanira kuchengetwa kure nechiedza uye inofanira kuchengetwa pa -20 ℃.Kana ikashandiswa kazhinji, inogona zvakare kuchengetwa pa4 ℃ kwenguva pfupi (mazuva gumi).
Komisheni yedu inofanirwa kunge iri yekushandira vashandisi vedu vekupedzisira nevatengi nemhando yepamusoro yepamusoro uye inokwikwidza inotakurika zvigadzirwa zvedhijitari uye mhinduro dzeKutengesa Kutengesa kune Imwe-Nhanho Probe Rt-Qpcr Kit Yekutsvagisa Shandisa V5001 200 Rxn Chete, Nheyo yebhizinesi redu inowanzo kupa zvinhu zvemhando yepamusoro, masevhisi ane hunyanzvi, uye kutaura kwechokwadi.Gamuchirai vese vakaroorana kuti vaise odha yekuedza yekugadzira hukama hwesangano kwenguva refu.
Kutengesa Kutsva kweChina Taq DNA Polymerase uye Qpcr, Zvinhu zvedu zvinotengeswa pasi rose.Vatengi vedu vanogara vachigutsikana nemhando yedu yakavimbika, masevhisi anotungamirwa nevatengi uye mitengo yemakwikwi.Basa redu ndere "kuramba tichiwana kuvimbika kwako nekuzvipira kuedza kwedu mukuvandudza nguva dzose kwezvinhu zvedu nemasevhisi kuitira kuti tive nechokwadi chekugutsikana kwevashandisi vedu vekupedzisira, vatengi, vashandi, vatengesi uye nharaunda dzepasirese dzatinoshandira pamwe".
Hapana masaini ekuwedzera
1.Taq DNA Polymerase mukiti inorasikirwa nebasa rayo nekuda kwekuchengetedza kusina kunaka kana kupera kwekiti.
Kurudziro: Simbisa mamiriro ekuchengetedza ekiti;wedzerazve huwandu hwakakodzera hweTaq DNA Polymerase kuPCR system kana kutenga itsva Real Time PCR Kit yezviedzo zvinoenderana.
2.Kune akawanda inhibitors yeTaq DNA Polymerase muDNA template.
Zvaungaita: Natsazve template kana kuderedza huwandu hwetemplate yakashandiswa.
3.Mg2 + concentration haina kukodzera.
Kurudziro: Iyo Mg2 + yekumisikidza ye2 × Real PCR Mix yatinopa ndeye 3.5mM.Nekudaro, kune mamwe akakosha ekutanga uye matemplate, iyo Mg2 + yekumisikidza inogona kunge yakakwira.Naizvozvo, unogona kuwedzera zvakananga MgCl2 kukwidziridza iyo Mg2+ concentration.Zvinokurudzirwa kuwedzera iyo Mg2+ 0.5mM nguva yega yega kuitira optimization.
4.Iyo PCR amplification mamiriro haana kukodzera, uye kutevedzana kweprimer kana kuisa pfungwa hakuna kunaka.
Mazano: simbisa kururamisa kwekutevedzana kweprimer uye primer haina kuderedzwa;kana chiratidzo chekusimudzira chisina kunaka, edza kudzikisira tembiricha yekumisa uye gadzirisa iyo primer concentration nenzira kwayo.
5.Kuwanda kwe template kuduku kana kuwanda.
Kurudziro: Ita template linearization gradient dilution, uye sarudza iyo template yekumisikidza ine yakanakisa PCR mhedzisiro yeChaiyo Nguva PCR kuyedza.
NTC ine yakawandisa fluorescence kukosha
1.Reagent kusvibiswa kunokonzerwa panguva yekushanda.
Kurudziro: Tsiva nema reagents eChaiyo Nguva PCR kuyedza.
2.Kusvibiswa kwakaitika panguva yekugadzirira kwePCR reaction system.
Kurudziro: Tora matanho ekudzivirira anodiwa panguva yekushanda, senge: kupfeka magurovhosi e latex, kushandisa piipi tipi ine sefa, nezvimwe.
3.The primers yakasvibiswa, uye kuderedzwa kwezvinyorwa kuchaita kuti kusava kwakananga amplification.
Kurudziro: Shandisa SDS-PAGE electrophoresis kuona kana maprimers akashatiswa, woatsiva nemaprimer matsva eChaiyo Nguva PCR kuyedza.
Primer dimer kana kuti non-specific amplification
1.Mg2 + concentration haina kukodzera.
Kurudziro: Iyo Mg2 + yepakati ye2 × Real PCR EasyTM Mix yatinopa ndeye 3.5 mM.Nekudaro, kune mamwe akakosha ekutanga uye matemplate, iyo Mg2 + yekumisikidza inogona kunge yakakwira.Naizvozvo, unogona kuwedzera zvakananga MgCl2 kukwidziridza iyo Mg2+ concentration.Zvinokurudzirwa kuwedzera iyo Mg2+ 0.5mM nguva yega yega kuitira optimization.
2.The PCR annealing tembiricha yakaderera.
Zano: Wedzera tembiricha yePCR annealing ne1 ℃ kana 2 ℃ nguva yega yega.
3.Chigadzirwa chePCR chakareba.
Kurudziro: Hurefu hweiyo Real Time PCR chigadzirwa hunofanirwa kunge huri pakati pe100-150bp, kwete kupfuura 500bp.
4.The primers yakasvibiswa, uye kusvibiswa kwezvinyorwa zvinotungamirira pakuonekwa kwekuwedzerwa kwakananga.
Kurudziro: Shandisa SDS-PAGE electrophoresis kuona kana maprimers akashatiswa, woatsiva nemaprimer matsva eChaiyo Nguva PCR kuyedza.
5.Iyo PCR system haina kunaka, kana hurongwa hwakanyanya kuduku.
Zano: Iyo PCR reaction system idiki zvakanyanya inokonzeresa kuti yekuona iderere.Zvakanakisa kushandisa reaction system inokurudzirwa neakawanda PCR chiridzwa kumhanyisa zvakare Yechokwadi Nguva PCR kuyedza.
Kusagona kudzokororwa kwehuwandu hunokosha
1.Chiridzwa chiri kutadza kushanda.
Zano: Panogona kunge paine kukanganisa pakati pegomba rega rega rePCR yechiridzwa, zvichikonzera kusaberekana zvakanaka panguva yekutarisira tembiricha kana kuona.Ndokumbira utarise maererano nemirairo yechiridzwa chinoenderana.
2.Kuchena kwemuenzaniso hakuna kunaka.
Kurudziro: Samples dzisina kuchena dzinotungamira mukutadza kuberekana kwekuyedza, izvo zvinosanganisira kuchena kwetemplate uye yekutanga.Zvakanakisa kuchenesa template, uye iyo yekutanga inonatswa zvakanyanya neSDS-PAGE.
3.Iyo PCR system yekugadzirira uye nguva yekuchengetedza yakanyanyisa.
Kurudziro: Shandisa iyo Real Time PCR system yePCR kuyedza nekukurumidza mushure mekugadzirira, uye usaisiye parutivi kwenguva yakareba.
4.Iyo PCR amplification mamiriro haana kukodzera, uye kutevedzana kweprimer kana kuisa pfungwa hakuna kunaka.
Mazano: simbisa kururamisa kwekutevedzana kweprimer uye primer haina kuderedzwa;kana chiratidzo chekusimudzira chisina kunaka, edza kudzikisira tembiricha yekumisa uye gadzirisa iyo primer concentration nenzira kwayo.
5.Iyo PCR system haina kunaka, kana hurongwa hwakanyanya kuduku.
Zano: Iyo PCR reaction system idiki zvakanyanya inokonzeresa kuti yekuona iderere.Zvakanakisa kushandisa reaction system inokurudzirwa neakawanda PCR chiridzwa kumhanyisa zvakare Yechokwadi Nguva PCR kuyedza.
