fekitori Zvitoro zveChina Yakanyanya Kushanda PCR, Taq Plus DNA Polymerase
Kit Description:
◮Yakanyanya kujeka: Iyo enzyme ine imwe inopisa-yekutanga chiitiko.
◮Kukurumidza Kuwedzera: 10 sec/kb.
◮Yakanyanya kuchinjika template: inogona kushandiswa kunyatso kusimudzira GC Yakakwirira kukosha, akasiyana-siyana akaoma-ku-kurisa DNA template.
◮Kuvimbika kwakasimba: yakajairwa Taq Enzyme 6 nguva.
◮Kugadzikana kwekushisa kwakasimba: Inogona kuiswa pa 37 ° C kwevhiki uye inochengetedza zvinopfuura 90% chiitiko.
Tichifunga nezve motto iyi, tashanduka kuita imwe yevanonyanya kugadzira tekinoroji, inodhura-inoshanda, uye inokwikwidza mutengo vagadziri vefekitori Zvigadzirwa zveChina High Efficiency PCR, Taq Plus DNA Polymerase, Kufemu yedu ine mhando yemhando yepamusoro yekutanga semotto yedu, tinogadzira zvigadzirwa zvinogadzirwa zvachose muJapan, kubva pakutenga zvinhu.Izvi zvinoita kuti vakwanise kushandiswa vaine rugare rwepfungwa.
Tichifunga nezve motto iyi, tave imwe yevanonyanya kugadzira tekinoroji, inodhura-inoshanda, uye inokwikwidza mutengo vagadziriChina PCR Amplification, Qpcr, Basa, Kuzvipira nguva dzose kwakakosha kuchinangwa chedu.Isu tagara tichienderana nekushandira vatengi, kugadzira kukosha manejimendi zvinangwa uye kuomerera kune kutendeseka, kuzvipira, inoshingirira manejimendi zano.
Zvinotsanangurwa
50×20μl rxns,200×20μl rxns,1000×20μl rxns, 2000×20μl rxns
The 2X Real PCR Easy TM Mix-Taqman inopiwa neReal Time PCR EasyTM-Taqman kit is a new premix system inoshandisa chaiyo fluorescent probes yeReal Time PCR amplification reactions, iyo inogona kuvandudza zvakanyanya chigadzirwa uye maitiro ekunzwa.ROX inopihwa seyemukati yekudzora dhayi.
2X Real PCR EasyTM Mix-Taqman ine Forgene yakasarudzika inopisa-kutanga Taq DNA Polymerase.Kuenzaniswa neakajairwa maTaq enzymes, ine mabhenefiti ekukwirira kwepamusoro, kugona kwakasimba kwekusimudzira uye yakaderera mismatch mwero.Inogona kuderedza kusiri-chaiyo amplification uye kunatsiridza huchokwadi hwePCR.
Kit zvikamu
| 2 × Real PCR EasyTM Mix-Taqman |
| 20 × ROX Reference Dye |
| DNase-Yemahara ddH2O |
| Mirayiridzo |
Zvimiro&zvakanakira
■ Nyore—2X PCR Sanganisa kuderedza chikanganiso chekuedza uye nguva yekushandisa
■ Yakananga-yakagadziridzwa buffer uye inopisa-kutanga Taq enzyme inogona kudzivirira isiri-chaiyo amplification uye primer dimer kugadzirwa.
■ Kunzwa kwakanyanya-inokwanisa kuona makopi akaderera etemplate
■ Kugona kuita zvakasiyana-siyana-kunoenderana nezvizhinji-chaiyo-chaiyo-chaiyo-chaiyo-yehuwandu PCR zviridzwa
Kit application
qPCR ongororo
Kuyerera Kwebasa
Graphic
Storage uye Sherufu upenyu
Iyi kit inofanira kuchengetwa kure nechiedza uye inofanira kuchengetwa pa -20 ℃.Kana ikashandiswa kakawanda, inogona zvakare kuchengetwa pa4℃ kwenguva pfupi (mazuva gumi). Tichifunga nezve motto iyi, tashanduka kuita imwe yevanonyanya kugadzira tekinoroji, inodhura-inoshanda, uye inokwikwidza mutengo vagadziri vefekitori Outlets yeChina High Efficiency PCR, Taq Plus DNA Polymerase, to 2503 yedu yekugadzira DNA, totanga ne2503 yedu yemhando yepamusoro, totanga pa2503 mokugadzira DNA yedu zvigadzirwa zvinogadzirwa zvachose muJapan, kubva pakutenga zvinhu kusvika pakugadziriswa.Izvi zvinoita kuti vakwanise kushandiswa vaine rugare rwepfungwa.
factory Zvitengesi zveChina PCR Amplification, Qpcr, Basa, Kuzvipira nguva dzose kwakakosha kuchinangwa chedu.Isu tagara tichienderana nekushandira vatengi, kugadzira kukosha manejimendi zvinangwa uye kuomerera kune kutendeseka, kuzvipira, inoshingirira manejimendi zano.
Hapana masaini ekuwedzera
1.Taq DNA Polymerase mukiti inorasikirwa nebasa rayo nekuda kwekuchengetedza kusina kunaka kana kupera kwekiti.
Kurudziro: Simbisa mamiriro ekuchengetedza ekiti;wedzerazve huwandu hwakakodzera hweTaq DNA Polymerase kuPCR system kana kutenga itsva Real Time PCR Kit yezviedzo zvinoenderana.
2.Kune akawanda inhibitors yeTaq DNA Polymerase muDNA template.
Zvaungaita: Natsazve template kana kuderedza huwandu hwetemplate yakashandiswa.
3.Mg2 + concentration haina kukodzera.
Kurudziro: Iyo Mg2 + yekumisikidza ye2 × Real PCR Mix yatinopa ndeye 3.5mM.Nekudaro, kune mamwe akakosha ekutanga uye matemplate, iyo Mg2 + yekumisikidza inogona kunge yakakwira.Naizvozvo, unogona kuwedzera zvakananga MgCl2 kukwidziridza iyo Mg2+ concentration.Zvinokurudzirwa kuwedzera iyo Mg2+ 0.5mM nguva yega yega kuitira optimization.
4.Iyo PCR amplification mamiriro haana kukodzera, uye kutevedzana kweprimer kana kuisa pfungwa hakuna kunaka.
Mazano: simbisa kururamisa kwekutevedzana kweprimer uye primer haina kuderedzwa;kana chiratidzo chekusimudzira chisina kunaka, edza kudzikisira tembiricha yekumisa uye gadzirisa iyo primer concentration nenzira kwayo.
5.Kuwanda kwe template kuduku kana kuwanda.
Kurudziro: Ita template linearization gradient dilution, uye sarudza iyo template yekumisikidza ine yakanakisa PCR mhedzisiro yeChaiyo Nguva PCR kuyedza.
NTC ine yakawandisa fluorescence kukosha
1.Reagent kusvibiswa kunokonzerwa panguva yekushanda.
Kurudziro: Tsiva nema reagents eChaiyo Nguva PCR kuyedza.
2.Kusvibiswa kwakaitika panguva yekugadzirira kwePCR reaction system.
Kurudziro: Tora matanho ekudzivirira anodiwa panguva yekushanda, senge: kupfeka magurovhosi e latex, kushandisa piipi tipi ine sefa, nezvimwe.
3.The primers yakasvibiswa, uye kuderedzwa kwezvinyorwa kuchaita kuti kusava kwakananga amplification.
Kurudziro: Shandisa SDS-PAGE electrophoresis kuona kana maprimers akashatiswa, woatsiva nemaprimer matsva eChaiyo Nguva PCR kuyedza.
Primer dimer kana kuti non-specific amplification
1.Mg2 + concentration haina kukodzera.
Kurudziro: Iyo Mg2 + yepakati ye2 × Real PCR EasyTM Mix yatinopa ndeye 3.5 mM.Nekudaro, kune mamwe akakosha ekutanga uye matemplate, iyo Mg2 + yekumisikidza inogona kunge yakakwira.Naizvozvo, unogona kuwedzera zvakananga MgCl2 kukwidziridza iyo Mg2+ concentration.Zvinokurudzirwa kuwedzera iyo Mg2+ 0.5mM nguva yega yega kuitira optimization.
2.The PCR annealing tembiricha yakaderera.
Zano: Wedzera tembiricha yePCR annealing ne1 ℃ kana 2 ℃ nguva yega yega.
3.Chigadzirwa chePCR chakareba.
Kurudziro: Hurefu hweiyo Real Time PCR chigadzirwa hunofanirwa kunge huri pakati pe100-150bp, kwete kupfuura 500bp.
4.The primers yakasvibiswa, uye kusvibiswa kwezvinyorwa zvinotungamirira pakuonekwa kwekuwedzerwa kwakananga.
Kurudziro: Shandisa SDS-PAGE electrophoresis kuona kana maprimers akashatiswa, woatsiva nemaprimer matsva eChaiyo Nguva PCR kuyedza.
5.Iyo PCR system haina kunaka, kana hurongwa hwakanyanya kuduku.
Zano: Iyo PCR reaction system idiki zvakanyanya inokonzeresa kuti yekuona iderere.Zvakanakisa kushandisa reaction system inokurudzirwa neakawanda PCR chiridzwa kumhanyisa zvakare Yechokwadi Nguva PCR kuyedza.
Kusagona kudzokororwa kwehuwandu hunokosha
1.Chiridzwa chiri kutadza kushanda.
Zano: Panogona kunge paine kukanganisa pakati pegomba rega rega rePCR yechiridzwa, zvichikonzera kusaberekana zvakanaka panguva yekutarisira tembiricha kana kuona.Ndokumbira utarise maererano nemirairo yechiridzwa chinoenderana.
2.Kuchena kwemuenzaniso hakuna kunaka.
Kurudziro: Samples dzisina kuchena dzinotungamira mukutadza kuberekana kwekuyedza, izvo zvinosanganisira kuchena kwetemplate uye yekutanga.Zvakanakisa kuchenesa template, uye iyo yekutanga inonatswa zvakanyanya neSDS-PAGE.
3.Iyo PCR system yekugadzirira uye nguva yekuchengetedza yakanyanyisa.
Kurudziro: Shandisa iyo Real Time PCR system yePCR kuyedza nekukurumidza mushure mekugadzirira, uye usaisiye parutivi kwenguva yakareba.
4.Iyo PCR amplification mamiriro haana kukodzera, uye kutevedzana kweprimer kana kuisa pfungwa hakuna kunaka.
Mazano: simbisa kururamisa kwekutevedzana kweprimer uye primer haina kuderedzwa;kana chiratidzo chekusimudzira chisina kunaka, edza kudzikisira tembiricha yekumisa uye gadzirisa iyo primer concentration nenzira kwayo.
5.Iyo PCR system haina kunaka, kana hurongwa hwakanyanya kuduku.
Zano: Iyo PCR reaction system idiki zvakanyanya inokonzeresa kuti yekuona iderere.Zvakanakisa kushandisa reaction system inokurudzirwa neakawanda PCR chiridzwa kumhanyisa zvakare Yechokwadi Nguva PCR kuyedza.
