Yakananga RT-qPCR Kit
Kit Description:
◮ Yakananga RT-qPCR Kit, uchishandisa Foreasy Reverse Transcriptase uye Foreasy HS Taq DNA Polymerase inogadzirwa neForegene, ine yakasarudzika reaction buffer, inoita kuti kit iyi ive nekupikisa kwakasimba uye kuenderana, uye inogona kuyedza zvakananga kuita kushandisa Foregene Lysis system setemplate.Iyi kit inogona kunyorera COVID-19 nucleic acid yekuona kit uye mamwe pathogenic bhakitiriya nucleic acid yekuona kit yekuvandudza.
Tsanangudzo
Yakananga RT-qPCR Kit inopa yakarongedzerwa RT-qPCR buffer uye yakakwira inoshanda enzyme.musanganiswa (Taq, M-MLV, RI), zvichiita kuti zvive nyore kugadzirwa kwemashure-ekupedzisira kits uye system.kugadzirisa, mukati mechokwadi chechokwadi.
Zvinotsanangurwa
500T, 50,000T
Kit zvikamu
| Kit zviri mukati (20 μL reaction system) | IM-05111-01 | IM-05112-01 |
| 500 T | 50,000 T | |
| Direct Enzyme Musanganiswa | 500 μL×1 | 50 mL×1 |
| 2 × Yakananga RT-qPCR Buffer | 1 mL×5 | 500 mL×1 |
| Instruction | 1 | 1 |
Matanho Okushanda
Pamberi pekugadzirira reagent, mareagents ese ari mukiti anofanirwa kunyungudutswa pakushambidzika kwekamuri uye kusanganiswa zvinyoro.
A: Gadzirira of template uye reagent
1. Gadzirira yakagadzirirwa RNA template (Inokurudzirwa kushandisa Foregene Total RNA Isolation Kit series kit kuti ubvise uye uchenese RNA template) kana sampuli yekugadzira chigadzirwa (Inokurudzirwa kushandisa Foregene Lysis system), mapeji chaiwo (10 μM) uye zvimwe zvinoshandiswa zvinoshandiswa, chiridzwa.
2. Isa iyo 2 × Yakananga RT-qPCR Buffer, RNase-Mahara ddH2O uye 20 × ROX Reference Dye (kana's inodiwa) mubhokisi nechando, zvichiita kuti izvi zvinyunguduke, uye zvakasanganiswa zvinyoro.
B: Gadzirira of RT-qPCR system
Tafura 1 : Gadzirira of RT-qPCR ssystem
| Chikamu | Volume | Final concentration |
| 2 × Yakananga RT-qPCR Buffer | 10 μL | 1× |
| Direct Enzyme Musanganiswa | 1μL | |
| Pamberi Pekutanga (10 μM) | 0.8 μL | 50-900nM |
| Reverse Primer(10 μM) | 0.8 μL | 50-900nM |
| Probe (4 μM) | 1μL | 200nM |
| Template (RNA kana Lysate) | X μL | |
| 20 × ROX Reference Dye | - | 1* |
| RNase-FreeddH2O | (6.4-X) μL | |
| Total Volume | 20μL |
C: Set the RT-qPCR reaction program
Tafura 2 : RT-qPCR reaction program setting (nokuti muenzaniso: piri-step nzira)
| Danho | Tembiricha | Nguva | Cycles | Content | |
| 1 | 50 ℃ | 15 min | 1* | 1 | Reverse transcription |
| 2 | 95℃ | 1 min | 1 | Pre-denaturation | |
| 3 | 95℃ | 10 sec | 40-45 | Denaturation | |
| 60 ℃ | 30 sec 2* | Anneal/Kuwedzera | |||
Storage uye Sherufu upenyu
-Kiti inofanira kuchengetwa pa -20± 5℃.Nguva yekushanda ndeye 12 mwedzi.
-Dzivisa kudzokororwa kwechando-thaw kutenderera (<5 kutenderera).
