ForeDirect RT-qPCR Kit
Kit Description:
◮YeRNA yakananga chaiyo-nguva amplification kubva swab kuunganidzwa pasina RNA yekuchenesa maitiro.Iyi kit inopedzisa qRT-PCR kutenderera mukati meawa imwe.Iyo enzyme musanganiswa musanganiswa wakagadziridzwa weReverse Transcriptase, Hot-Start Taq DNA polymerase, RNase inhibitor.Iyo Reaction Buffer ine zvese zvinodikanwa zvikamu, zvinosanganisira optimized buffer zvikamu, Mg2+, dUTP, uye dNTPs.
Tsanangudzo
ForeDirect RT-qPCR Kit yakagadzirirwa RNA yakananga chaiyo-nguva amplification kubva swab kuunganidzwa pasina RNA yekucheneswa maitiro.Iyi kit inopedzisa qRT-PCR kutenderera mukati meawa imwe.Iyo enzyme musanganiswa musanganiswa wakagadziridzwa weReverse Transcriptase, Hot-Start Taq DNA polymerase, RNase inhibitor.Iyo Reaction Buffer ine zvese zvinodikanwa zvikamu, zvinosanganisira optimized buffer zvikamu, Mg2+, dUTP, uye dNTPs.
Zvinotsanangurwa
100T
Kit zvikamu
| Nucleic acid kuburitsa agent |
| RNA inodzivirira |
| 2 × RT-qPCR Buffer |
| Enzyme Musanganiswa(Taq&M-MLV) |
| Mirayiridzo |
PCR application
1. Kana PCR reaction chubhu dzave pfupi centrifuged, dziise mumuenzaniso tangi yechiridzwa amplification.
2. Thermal protocol
| Danho | Tembiricha | Nguva | Cycles | |
| 1 | Reverse transcription | 50 ℃ | 15 mins | 1 |
| 2 | Pre-denaturation | 95℃ | 1 min | 1 |
| 3 | Denaturation | 95℃ | 10 seconds | 42 |
| 4 | Anneal/Kuwedzera | 60 ℃ | 30 seconds | |
Cherechedza:Chiratidzo chefluorescent chakaonekwa pakarepo mushure mekuwedzera danho rekutenderera kwega.
3. Mushure mekugadzirisa, chengetedza faira uye shandisa purogiramu yekuita.
Matanho Okushanda
Pamberi pekugadzirira reagent, mareagents ese ari mukiti anofanirwa kunyungudutswa pakushambidzika kwekamuri uye kusanganiswa zvinyoro.
Reagent kugadzirira
1. Gadzirira Nucleic Acid Release Mix
| Chikamu | Vhoriyamu paSample kana Kudzora |
| Nucleic acid kuburitsa agent | 5μL |
| RNA inodzivirira | 0.5 μL |
2. Gadzirira Reaction Mix
| Chikamu | Vhoriyamu paSample kana Kudzora |
| 2 × RT-qPCR Buffer | 15 μL |
| Enzyme Musanganiswa(Taq&M-MLV) | 1.5 μL |
| Primers uye Probes | 1μL |
Storage uye Sherufu upenyu
-Kiti inofanira kuchengetwa pa -20± 5℃.Nguva yekushanda ndeye 12 mwedzi.
-Dzivisa kudzokororwa kwechando-thaw kutenderera (<5 kutenderera).
