Wholesale Dealers of China Techstar Magnetic Bead Method Swab Sample Nucleic Acid Extraction Kit Rna Isolation Reagent DNA Purification Kit
Kit Description:
RNase-Yemahara
Inoshanda pakamuri tembiricha (15-25 ℃)
NeDNA-Kuchenesa Column
High RNA goho
Kutsanya:Pedzisa kudhirowa mumaminitsi gumi nerimwe
Kuchengetedza: Hapana Organic makemikari
Sangano rinotsigira huzivi hwe "Iva No.1 muhutano hwakanaka, dzika midzi panhoroondo yechikwereti uye kuvimbika kwekukura", icharamba ichipa vatengi vekare uye vatsva kubva kumusha uye mhiri kwemakungwa zvizere-kupisa kune Vatengesi veHolesale yeChina Techstar Magnetic Bead Method Swab Sample Nucleic Acid Extraction Kit Rna Isolation Reagent , DNA Isolation Reagent , DNA yakanaka uye tive negoti tinogamuchira bhizinesi duku, DNA negoti .Isu tinotarisira kurovanisa maoko neshamwari mumaindasitiri akasiyana kuti tigadzire yakanakisa inouya.
Sangano rinotsigira huzivi hwe "Iva No.1 muhutano hwakanaka, dzika midzi panhoroondo yechikwereti uye kuvimbika kwekukura", icharamba ichipa vatengi vekare uye vatsva kubva kumba uye mhiri kwemakungwa zvizere.China Nucleic Acid Reagent, DNA/Rna Extraction Kit, Nekukura kwekambani, ikozvino zvigadzirwa zvedu zvinotengeswa uye zvakashanda kune dzinopfuura 15 nyika pasi rose, seEurope, North America, Middle-East, South America, Southern Asia uye zvichingodaro.Sezvatinorangarira mupfungwa dzedu kuti hutsva hwakakosha mukukura kwedu, kuvandudzwa kwechigadzirwa kutsva nguva dzose.Besides, Maitiro edu anochinjika uye anoshanda anoshanda,Zvigadzirwa zvemhando yepamusoro uye mitengo yemakwikwi ndizvo chaizvo izvo vatengi vedu vari kutsvaga.Uyewo basa rakakura rinotiunzira mukurumbira wakanaka wechikwereti.
Tsanangudzo
Iyi kit inoshandisa spin column uye fomula yakagadziridzwa naForegene, iyo inogona kunyatso kuburitsa yakakwirira-kuchena uye yemhando yepamusoro RNA kubva kumaseru akagadzirwa mu96, 24, 12, uye 6-matsime mahwendefa.
Iyo kit inopa inoshanda DNA-Kuchenesa Column, iyo inogona nyore kuparadzanisa supernatant uye sero lysate, kusunga uye kubvisa genomic DNA.Kuvhiya kuri nyore uye kunochengetedza nguva.
Iyo RNA-chete Column inogona kunyatso kusunga RNA neyakasiyana fomula.Nhamba huru yemasampuli inogona kugadziriswa panguva imwe chete.
Kit zvikamu
| Kit composition | RE-03111 | RE-03114 |
| 50 T | 200 T | |
| Buffer cRL1* | 25 mL | 100 mL |
| Buffer cRL2 | 15 mL | 60 mL |
| Buffer RW1* | 25 mL | 100 mL |
| Buffer RW2 | 24 mL | 96 mL |
| RNase-Yemahara ddH2O | 10 mL | 40 mL |
| RNA-Chete Column | 50 | 200 |
| DNA-Kuchenesa Column | 50 | 200 |
| Instruction | 1 | 1 |
* Ndokumbira upfeke magirovhosi uye tora matanho ekudzivirira panguva yekushanda sezvo Buffer cRL1 uye Buffer RW1 ine inogumbura chaotropic munyu.
Zvimiro&zvakanakira
∎ Iyo yese nzira inoshandiswa pakudziya kwekamuri (15-25 ℃), pasina kugeza echando uye yakaderera tembiricha centrifugation.
■ Kiti yese ndeyeRNase-Yemahara, hapana chikonzero chekunetseka nezveRNA kuparara.
■ DNA-Cleaning Column inosunga zvakananga DNA, kuitira kuti kit ikwanise kubvisa genomic DNA kusvibiswa pasina kuwedzera imwe DNase.
■ Yakakwira RNA goho: RNA-chete Column uye yakasarudzika fomula inogona kunyatsochenesa RNA.
■ Kukurumidza kumhanya: nyore kushanda uye inogona kupedzwa mumaminitsi gumi nerimwe.
■ Chengetedzo: Hapana organic reagent inodiwa.
■ Hunhu hwepamusoro: RNA yakacheneswa ndeyekuchena kwepamusoro, haina mapuroteni uye kumwe kusvibiswa, uye inogona kusangana nemiedzo yakasiyana-siyana inotevera.
Kit application
Yakakodzera kudhirowa uye kucheneswa kweiyo RNA yakazara kubva kumasero akagadzirwa mu96, 24, 12, uye 6-matsime mahwendefa.
Kuyerera kwebasa
Mufananidzo
Iyo agarose gel bhatiri dhayagiramu yeSero Yese RNA Isolation Kit yakabata nhamba dziri pamusoro dzakasiyana dzemasero, 20μl vhoriyamu elution, tora 2μl yakacheneswa yakazara RNA 1%.
Kuchengetedza uye hupenyu hwesherufu
Iyo kit inogona kuchengetwa kwemwedzi gumi nemaviri pane tembiricha yekamuri (15-25 ℃) kana 2-8 ℃ kwenguva yakareba (24 mwedzi).
Buffer cRL1 inogona kuchengetwa pa 4 ℃ kwemwedzi 1 mushure mekuwedzera 2-hydroxy-1-ethanethiol(optional).Sangano rinotsigira huzivi hwe"Iva No.1 mumhando yakanaka, dzika midzi panhoroondo yechikwereti uye kuvimbika kwekukura", icharamba ichipa vatengi vekare uye vatsva kubva kumba nekumhiri kwemakungwa yose-heatedly yeChina Bead Exthocleation yeActhocles Kit Rna Isolation Reagent DNA Kuchenesa Kit, Isu tinogashira nemoyo wese shamwari dzakanaka kutaurirana bhizinesi diki uye kutanga kushandira pamwe nesu.Isu tinotarisira kurovanisa maoko neshamwari mumaindasitiri akasiyana kuti tigadzire yakanakisa inouya.
Vatengesi vezvitoro zveChina Nucleic Acid Reagent, DNA/Rna Extraction Kit, Nekukura kwekambani, ikozvino zvigadzirwa zvedu zvinotengeswa uye zvakashanda kune dzinopfuura 15 nyika pasi rose, seEurope, North America, Middle-East, South America, Southern Asia uye zvichingodaro.Sezvatinorangarira mupfungwa dzedu kuti hutsva hwakakosha mukukura kwedu, kuvandudzwa kwechigadzirwa kutsva nguva dzose.Besides, Maitiro edu anochinjika uye anoshanda anoshanda,Zvigadzirwa zvemhando yepamusoro uye mitengo yemakwikwi ndizvo chaizvo izvo vatengi vedu vari kutsvaga.Uyewo basa rakakura rinotiunzira mukurumbira wakanaka wechikwereti.
RNA haina kubviswa kana RNA goho rakaderera
Panowanzove nemhando dzakasiyana dzezvinhu zvinokanganisa kudzoreredza kushanda zvakanaka, senge: tishu sampuli RNA yemukati, nzira yekushanda, elution vhoriyamu, nezvimwe.
1. Ice bath kana cryogenic (4 ° C) centrifugation yakaitwa panguva yekushanda.
Kurudziro: Shanda pamhepo tembiricha (15-25 ° C) mukati mese muitiro, usaite ice yekugezesa uye centrifuge pane yakaderera tembiricha.
2. Kuchengetedzwa kwemuenzaniso kusina kukodzera kana nguva yakawandisa yekuchengetedza sampuli.
Kurudziro: Chengetedza samples pa -80 °C kana kuomesa munitrogen yemvura uye dzivirira kudzokororwa kushandiswa kwechando-nyoro;edza kushandisa matishu matsva kana maseru akagadzirwa eRNA kutorwa.
3. Kusakwana sampuli lysis.
Kurudziro: Paunenge uchigadzira matishu, ita shuwa kuti matishu akanyatsoenderana uye kuti masero ematishu akapatsanurwa zvakakwana kutsanangura kuburitswa kweRNA.
4. Iyo enzi haina kuwedzerwa nemazvo.
Kurudziro: Simbisa kuti RNase-Yemahara ddH2O inowedzerwa kudonha kusvika pakati peyekucheneswa column membrane.
5. Vhoriyamu chaiyo yeabsolute ethanol haina kuwedzerwa kuBuffer RL2 kana Buffer RW2.
Kurudziro: Tevedzera mirairo, wedzera iyo chaiyo vhoriyamu yeabsolute ethanol kuBuffer RL2 uye Buffer RW2 uye sanganisa zvakanaka usati washandisa kiti.
6. Tissue sample dosage haina kukodzera.
Kurudziro: Shandisa 10-20 mg yetishu kana (1-5) × 106masero pa 500 μl buffer RL1, sezvo kushandiswa kwematishu zvakanyanya kunogona kukonzera kuderedzwa kweRNA.
7. Vhoriyamu yakashata isina kunaka kana kuti kuburitsa kusina kukwana.
Kurudziro: Vhoriyamu yekujekesa yekoramu yekuchenesa ndeye 50-200 μl;kana iyo elution mhedzisiro isingagutsi, zvinokurudzirwa kuwedzera tembiricha yekuisa yekamuri nguva mushure mekuwedzera preheated RNase-Mahara ddH.2O, eg kwe5-10 min.
8.Koramu yekuchenesa ine ethanol yakasara mushure meBuffer RW2 wash.
Kurudziro: Kana paine ethanol yakasara mushure mekugezwa kweBuffer RW2, isina chinhu chubhu centrifugation ye1min, nguva yekushayikwa kwechubhu centrifugation operation inogona kuwedzerwa kusvika 2min, kana mbiru yekuchenesa inogona kuiswa pakushambidzika kwemaminetsi mashanu kuti ibvise zvakakwana ethanol yakasara.
Yakanatswa RNA yakaderedzwa
Hunhu hweRNA yakacheneswa hune hukama nezvinhu zvakaita sekuchengetedza sampuli, kusvibiswa kweRNase, uye kunyengera, nezvimwe.
1. Tissue samples haisi kuchengetwa munguva.
Kurudziro: Kana masamples ematishu kana maseru akasashandiswa nenguva mushure mekuunganidzwa, pakarepo cryopreserve pa -80 ° C kana liquid nitrogen.Kuti ubvise RNA, shandisa ichangobva kutorwa tishu kana sero sampuli pese pazvinogoneka.
2. Kudzokororwa kwechando-kunyunguduka kwemasampuli ematishu.
Kurudziro: Paunenge uchichengeta matishu samples, zviri nani kuacheka kuita zvidimbu zvidiki kuti zvichengetedzwe, uye kubvisa chimwe chezvidimbu paunenge uchizvishandisa kudzivirira kudzokororwa nechando-kunyunguduka kwesample uye kushatiswa kweRNA.
3. RNase inounzwa kana kusapfeka magirovhosi anoraswa, masiki, nezvimwewo panguva yekushanda.
Kurudziro: RNA yekuwedzera yekuedza inoitwa zvakanyanya mumakamuri akasiyana eRNA manipulation uye tafura inocheneswa isati yaedzwa.
Pfeka magurovhosi anoraswa uye masks panguva yekuyedza kuderedza kuderedzwa kweRNA kunokonzerwa nekuunzwa kweRNase.
4. Zvigadzirwa zvakasvibiswa neRNase panguva yekushandiswa.
Kurudziro: Tsiva neMhuka Yakazara RNA Isolation Kit yezviyedzo zvinoenderana.
5. Machubhu ecentrifuge, mazano, nezvimwewo zvinoshandiswa muRNA manipulation zvakasvibiswa neRNase.
Kurudziro: Simbisa kuti machubhu ecentrifuge, matipi, mapipette, nezvimwewo anoshandiswa muRNA kudhirowa ese RNase-Mahara.
Yakacheneswa yakawanikwa RNA inokanganisa kuyedza kwepasi
RNA yakacheneswa nekoramu yekuchenesa, kana maion emunyu, mapuroteni akawandisa anozokanganisa kuyedza kwakadzika, senge: reverse transcription,Northern Blot et al.
1. Iyo elutioned RNA ine salt ion residues.
Kurudziro: Simbisa kuti vhoriyamu chaiyo ye ethanol yawedzerwa kuBuffer RW2 uye ita 2 mbiru yekunatsa washes pa centrifugal speed inoratidzwa kuti ishande;kana paine ion inosara yemunyu, siya mbiru yekuchenesa kuBuffer RW2 kwemaminetsi mashanu patembiricha yekamuri uye ita centrifugation kuti uwedzere kubviswa kwemunyu.
2. Ethanol yakasara mune elutioned RNA.
Recommendation: Simbisa kuti mushure mekugeza RW2 buffer, ita isina chinhu chubhu centrifugation operation pa centrifugation speed inoratidzwa kuti ishande, wedzera nguva yekushaikwa kwechubhu centrifugation oparesheni kusvika 2 min kana kuchine ethanol yasara, kana kuisiya patembiricha ye 5 m.
