Komisheni yedu inofanirwa kunge iri yekushandira vashandisi vedu vekupedzisira nevatengi vane yakanakisa mhando yepamusoro uye inokwikwidza inotakurika zvigadzirwa zvedhijitari uye mhinduro dze.PCR Reagent, Isu tinotambira nemoyo wese vaviri vekunze uye vekumba bhizinesi bhizinesi vanobatana, uye tinotarisira kushanda pamwe newe mukati menguva yakareba!
Komisheni yedu inofanirwa kunge iri yekushandira vashandisi vedu vekupedzisira nevatengi vane yakanakisa mhando yepamusoro uye inokwikwidza inotakurika zvigadzirwa zvedhijitari uye mhinduro dze.PCR Reagent, Neanoda kusvika makore makumi matatu 'ruzivo mubhizinesi, tanga tichivimba nepamusoro sevhisi, mhando uye kuendesa.Isu tinotambira neushamwari vatengi vanobva kumativi ese enyika kuti tibatane nekambani yedu mukusimudzira kwakafanana.

Zvinotsanangurwa

5ml, 10ml, 40ml

Kit zvikamu

Zvimiro&zvakanakira

■ Kuvimbika kwepamusoro: kakapetwa ka6 kuya kweye enzyme yeTaqwo zvayo;

■ Inokurumidza amplification sipidhi : sipidhi yeamplification ndeye 5-10sec/kb, inova ka6-12 kakapetwa ka12 kune yakajairika Taq enzyme;

■ Kuwedzera kuchinjika kwetemplate: Inogona kukudza zvakasiyana-siyana zvakaoma matemplate eDNA nepamusoroGC zvemukati;

∎ Kukwirisa kwepamusoro: mitsetse yekusimudzira yakaderera pane yakajairika Taq enzyme;

■ Kushivirira kwezvakatipoteredza kwakasimba: kuiswa pa 37 ° C kwevhiki, kuchengetedza zvinopfuura 90% basa;

■ Iine 5′ →3′ DNA polymerase basa uye 5′→3′ exonuclease basa, pasina 3′ →5′ exonuclease basa.

■ Iyo kititi yakagara yakasanganiswa neElectrophoresis Loading Buffer, mushure mokunge PCR yapera, sampu inogona kutakurwa zvakananga kune electrophoresis;kushanda kuri nyore uye nyore.

Kit application

PCR kukwidziridzwa kwezvimedu zveDNA, kunyora DNA, DNA sequencing,PCR pamwe nemuswe

Storage uye Sherufu upenyu

Inogona kuchengetwa kwenguva yakareba pa -20 ° C;nokuda kwekushandisa nguva dzose, inogona kuchengetwa pa 4 ° C kwenguva pfupi.The 2 × Real PCR Easy TM Mix-SYBR inopiwa ne Real Time PCR EasyTM-SYBR Green I kit is a new premix system inoshandisa SYBR Green I for Real Time PCR amplification reactions, iyo inogona kuvandudza zvakanyanya chigadzirwa uye kunzwisiswa kwekunzwa .ROX inopihwa seyemukati yekudzora dhayi.Iyo fluorescence intensity yekiti iyi ndeye 3-5 nguva yezvigadzirwa zvakafanana, izvo zvinogona kuve zvakanyanya kunzwisisika uye intuitively kuratidza kusungwa kwechinangwa template DNA.
2×Real PCR EasyTM Mix-SYBR ine Forgene yakasarudzika inopisa-kutanga Taq DNA Polymerase.Kuenzaniswa neakajairwa maTaq enzymes, ine mabhenefiti ekukwirira kwepamusoro, kugona kwakasimba kwekusimudzira uye yakaderera mismatch mwero.Inogona kuderedza kusiri-chaiyo amplification uye kunatsiridza huchokwadi hwePCR.