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pcr master mix

pcr master mix Featured Image
  • pcr master mix
  • pcr master mix
  • pcr master mix
  • pcr master mix

Kit Description:

Iyi kit inoshandisa yakasarudzika lysis buffer system kukurumidza kuburitsa genomic DNA kubva kuzebrafish uye mamwe matishu ehove yemvura yakachena, zvimbi zvemuswe kana mazai ehove samples for PCR reactions, saka inonyanya kukodzera kuongororwa kwakakura kwemajini.Iyo nzira yekuburitsa genomic DNA kubva kulysis buffer inopedzwa mukati me10-30 maminetsi pa65 ° C.Hapana mamwe maitiro akadai seprotein uye RNA kubviswa anodiwa, uye yakaburitswa trace DNA inogona kushandiswa setemplate yePCR reaction.

2 × PCR Easy TM Mix ine kushivirira kwakasimba kuPCR reaction inhibitors, uye inogona kushandisa lysate yemuenzaniso kuti iongororwe se template yekushanda uye yakananga amplification.Iyi reagent ine ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer uye stabilizer.Inoshandiswa pamwe chete ne lysis buffer inogona kukurumidza uye nyore kuona samples, uye ine hunhu hwekunzwa kwepamusoro, kusimba kwakasimba, uye kugadzikana kwakanaka.

D-Taq DNA polymerase ndeye DNA polymerase yakanyatso gadzirwa neForegene kune yakananga PCR maitiro.D-Taq DNA polymerase ine kushivirira kwakasimba kune akasiyana PCR reaction inhibitors, uye inokwanisa kunyatso kukwidziridza huwandu hweDNA mune dzakasiyana siyana dzakaomarara maitiro masisitimu, uye iyo amplification kumhanya inogona kusvika 2Kb/min, iyo inonyanya kukodzera Direct PCR reaction.


Product Detail

Product Tags

Tichaita nhamburiko yega yega kuti ive yakasarudzika uye yakanaka, uye nekumhanyisa nhanho dzedu dzekumira mukati mechikamu chepasirese epamusoro-giredhi uye epamusoro-tech mabhizinesi epcr master mix, Isu tanga tichidzingirira WIN-WIN dambudziko nevatengi vedu.Isu tinogashira neushamwari vatengi kubva kwese kwese pasirese vari kuuya kupfuura kushanya uye nekutanga kubatanidza kwenguva refu.
Tichaita nhamburiko yega yega kuti ive yakasarudzika uye yakanaka, uye nekumhanyisa nhanho dzedu dzekumira mukati mechikamu chepasirese epamusoro-giredhi uye epamusoro-tech mabhizinesi epcr master mix, Iyo kambani ine yakakwana manejimendi system uye mushure mekutengesa sevhisi system.Isu tinozvipira pakuvaka piyona muindasitiri yemasefa.Fekitori yedu inoda kushanda pamwe nevatengi vakasiyana vekumba nekumhiri kwemakungwa kuti vawane ramangwana riri nani uye riri nani.

Zvinotsanangurwa

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Kit zvikamu

Chikamu I

Buffer FP

Foregene Protease

6 × DNA Loading Buffer

Chikamu II

2 × PCR NyoreTMMix

Mirayiridzo

Zvimiro&zvakanakira

■ Hapana kunotora nguva uye kunodhura kuchenesa DNA kunodiwa.

∎ Sample inodikanwa idiki, zvishoma se1mg hove caudal fin kana 10 mazai ehove anogona kuongororwa.

∎ Hapana mishonga yakakosha yakadai sekukuya nekupwanya inodiwa, uye kuvhiya kuri nyore.

■ Optimized PCR system inoita kuti PCR ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.

Kit parameters

Kushandisa: Hove dzeZebra nedzimwe hove dzemvura yakachena.

DNA yakaburitswa kubva kumuenzaniso lysis: inoshandiswa chete sePCR template.

Iyo kit inogona kushandiswa kune zvinotevera zvinangwa: kuzivikanwa kwe transgenosis, mhuka genotyping, nezvimwe.

Kuyerera kwebasa

wk-flw-zebra-fish-direct-PCR

Storage uye Sherufu upenyu

Chikamu I chekiti ichi chinofanira kuchengetwa pa2-8 ℃.

Iyo reagent Buffer FP inogona kuchengetwa kwemwedzi gumi nemiviri pasi pemamiriro akaoma;inogona kuchengetwa pa2-8 ℃ kuitira kuchengetedza kwenguva refu.

v Reagent Forgene Protease ine yakasarudzika fomula.Kuti ive nechokwadi chekuita kwayo uye kugadzikana, ndapota chengeta pa4 ℃ kwemwedzi gumi nemiviri.

vReagent 6 × DNA Loading Buffer inogona kuchengetwa pa4 ℃ kana -20 ℃ kwenguva yakareba.

Chikamu II chekiti ichi chinofanira kuchengetwa pa -20 ℃.

vReagent 2 × PCR Easy TM Mix, kana inoshandiswa kakawanda, inogonawo kuchengetwa pa 4 ℃ yekuchengetedza kwenguva pfupi (shandisa mukati memazuva gumi).

Tichaita nhamburiko yega yega kuti ive yakasarudzika uye yakanaka, uye nekumhanyisa nhanho dzedu dzekumira mukati mechikamu chepasirese epamusoro-giredhi uye epamusoro-tech mabhizinesi eIOS Certificate China All Steel Radial Truck Matairi, Mabhazi Matai, TBR Matayi, Radial Matayi (315/80R22.5, 11R22.52.Isu tinogashira neushamwari vatengi kubva kwese kwese pasirese vari kuuya kupfuura kushanya uye nekutanga kubatanidza kwenguva refu.
IOS Chitupa China Rori Tire, Radial Rori Tire, Iyo kambani ine yakakwana manejimendi system uye mushure-yekutengesa sevhisi system.Isu tinozvipira pakuvaka piyona muindasitiri yemasefa.Fekitori yedu inoda kushanda pamwe nevatengi vakasiyana vekumba nekumhiri kwemakungwa kuti vawane ramangwana riri nani uye riri nani.

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