Kutsvaga kwedu uye chinangwa chekambani kugara chiri "Nguva dzose tichigutsa zvinodiwa nevatengi vedu".Isu tinoramba tichitora uye dhizaini nekugadzira zvinoshamisa zvemhando yepamusoro kune yega yega vatengi vedu vekare uye vatsva uye tinosvika pakuhwina-kuhwina tarisiro yevatengi vedu pamwe nesu yeOEM/ODM China Viral DNA & Rna Nucleic Acid Extraction Kit YeNguva Yechokwadi PCR, Isu tinoramba tichiwana bhizinesi mweya wedu "mhando inogara sangano, chikwereti chinovimbisa kushandira pamwe uye ramba uchichengeta pfungwa dzedu.
Kutsvaga kwedu uye chinangwa chekambani kugara chiri "Nguva dzose tichigutsa zvinodiwa nevatengi vedu".Isu tinoramba tichiwana uye dhizaini nekugadzira zvinoshamisa zvemhando yepamusoro zvigadzirwa kune yega yega vatengi vedu vekare uye vatsva uye tinosvika pakukunda-kuhwina tarisiro yevatengi vedu pamwe nesuChina Viral Rna/DNA Extraction Kit uye Magnetic Bead Rna&DNA Extraction Kit, Takawana ISO9001 iyo inopa hwaro hwakasimba hwekumwe budiriro yedu.Kurambira mu "High quality, Prompt Delivery, Competitive Price", takagadzira kushandira pamwe kwenguva refu nevatengi vanobva mhiri kwemakungwa uye vemunyika uye tinowana mhinduro dzepamusoro dzevatengi uye dzekare.Kukudzwa kwedu kukuru kusangana nezvido zvenyu.Tiri kutarisira kutarisisa kwenyu nomwoyo wose.
Mirayiridzo yezvinyorwa:

Kutsvaga kwedu uye chinangwa chekambani kugara chiri "Nguva dzose tichigutsa zvinodiwa nevatengi vedu".Isu tinoramba tichitora uye dhizaini nekugadzira zvinoshamisa zvemhando yepamusoro kune yega yega vatengi vedu vekare uye vatsva uye tinosvika pakuhwina-kuhwina tarisiro yevatengi vedu pamwe nesu yeOEM/ODM China Viral DNA & Rna Nucleic Acid Extraction Kit YeNguva Yechokwadi PCR, Isu tinoramba tichiwana bhizinesi mweya wedu "mhando inogara sangano, chikwereti chinovimbisa kushandira pamwe uye ramba uchichengeta pfungwa dzedu.
OEM/ODM China China Viral Rna/DNA Extraction Kit uye Magnetic Bead Rna&DNA Extraction Kit, Takawana ISO9001 iyo inopa hwaro hwakasimba hwekuenderera mberi kwedu budiriro.Kurambira mu "High quality, Prompt Delivery, Competitive Price", takagadzira kushandira pamwe kwenguva refu nevatengi vanobva mhiri kwemakungwa uye vemunyika uye tinowana mhinduro dzepamusoro dzevatengi uye dzekare.Kukudzwa kwedu kukuru kusangana nezvido zvenyu.Tiri kutarisira kutarisisa kwenyu nomwoyo wose.

Dambudziko rekuongorora Guide

Izvi zvinotevera kuongororwa kwezvinetso zvinogona kusangana mukubviswa kwehutachiona hweDNA / RNA, uchitarisira kubatsira kune zvaunoedza.Pamusoro pezvo, kune mamwe matambudziko ekuyedza kana ehunyanzvi kunze kwemirairo yekushanda uye kuongororwa kwedambudziko, isu takatsaurira tekinoroji rutsigiro kuti ikubatsire.Kana uine chero zvaunoda, tapota taura nesu: 028-83360257 kana E-mail:

Tech@foregene.com.

Hapana nucleic acid inotorwa kana yakaderera nucleic acid goho

Pane kazhinji zvinhu zvakawanda zvinokanganisa kugona kudzoreredza, senge: sampuli nucleic acid yemukati, nzira yekushandisa, elution vhoriyamu, nezvimwe.

Kuongororwa kwezvikonzero zvinowanzoitika:

1. Bhati yechando kana kutonhora kwakadzika (4 ° C) centrifugation yakaitwa panguva yekuita.

Zano: Shanda pane tembiricha yekamuri (15-25 ° C) mukati mese muitiro, usaite ice yekugezesa uye yakaderera tembiricha centrifugation.

2. Muenzaniso wakachengetwa zvisina kunaka kana sampuli yakachengetwa kwenguva yakareba.

Kurudziro: Chengetedza samples pa -80 ° C uye usadzore kutonhora nekunyunguduka;edza kushandisa zvichangobva kuunganidzwa sampuli kuti nucleic acid kutorwa.

3. Kusakwana sampuli lysis.

Kurudziro: Tapota onai kuti sampuli uye lysis kushanda mhinduro yakanyatsosanganiswa uye incubated pakamuri tembiricha (15-25 ° C) kwemaminitsi gumi.

4. Kuwedzera zvisirizvo kweeluent.

Kurudziro: Ita shuwa kuti RNase-Yemahara ddH2O inowedzerwa inodzika kusvika pakati pechenese column membrane, uye usadonhedze pakona yekuchenesa mhete.

5. Vhoriyamu chaiyo yeabsolute ethanol haina kuwedzerwa kuBuffer RW2.

Zano: Ndokumbira uteedzere mirairo, wedzera huwandu chaihwo hweabsolute ethanol kuBuffer RW2 uye sanganisa zvakanaka usati washandisa kit.

6. Sample vhoriyamu isina kufanira.

Zano: 200µl yesampuli inogadziridzwa kune yega yega 500µl yeBuffer DRL.Yakawandisa sampuli yekugadzirisa inozokonzera yakaderera nucleic acid yekubvisa goho.

7. Vhoriyamu yekuvhota isina kufanira kana kusakwana.

Kurudziro: Vhoriyamu yakasarudzika yekoramu yekuchenesa ndeye 30-50μl;kana iyo elution mhedzisiro isiri inogutsa, zvinokurudzirwa kuwedzera nguva pakamuri tembiricha mushure mekuwedzera preheated RNase-Mahara ddH2O, senge 5-10min.

8. Ethanol inoramba iri pambiru mushure mekugeza neBuffer RW2.

Zano: Kana ethanol ikasara mushure mekuisa centrifugation neBuffer RW2 kwemaminetsi maviri, iyo column inogona kuiswa pakamuri tembiricha kwemaminetsi mashanu mushure mekuita centrifugation kuti ibvise zvachose yakasara ethanol.

Iyo yakacheneswa nucleic acid yakasvibiswa

Hunhu hwekucheneswa nucleic acid hunoenderana nekuchengetedza sampuli, kusvibiswa kweRNase, kushanda uye zvimwe zvinhu.Kuongororwa kwezvikonzero zvinowanzoitika:

1. Masampuli akaunganidzwa haana kuchengetwa nenguva.

Zvaungaita: Kana sampuli ikasashandiswa munguva mushure mekuunganidza, tapota chengeta pa -80 ° C pakupisa kwakaderera pakarepo.Kune RNA kutorwa, edza kushandisa masampuli achangobva kuunganidzwa.

2. Unganidza sampuli uye omesa uye unyunguduke kakawanda.

Zano: Dzivisa kutonhora nekunyunguduka (kwete kanopfuura kamwe) panguva yekuunganidza uye kuchengetwa kwemasampuli, zvikasadaro iyo nucleic acid goho ichaderedzwa.

3. RNase inounzwa mukamuri yekuvhiya kana magurovhosi anoraswa, masks, nezvimwe hazvina kupfekwa.

Kurudziro: Maedzo ekubvisa eRNA anoitwa zvakanyanya mukamuri yekuvhiya yeRNA, uye tafura yemurabhoritari inofanirwa kucheneswa kusati kwaitwa.

Pfeka magurovhosi anoraswa uye masiki panguva yekuyedza kudzivirira kusvibiswa kweRNA kunokonzerwa nekuunzwa kweRNase zvakanyanya.

4. Iyo reagent yakasvibiswa neRNase panguva yekushandiswa.

Kurudziro: Tsiva neViral DNA/RNA Isolation Kit yezviyedzo zvine hukama.

5. Machubhu ecentrifuge nemapipetti anoshandiswa paRNA manipulation akasvibiswa neRNase.

Zvaungaita: Ita shuwa kuti machubhu ecentrifuge, matipi epipette, mapipetti, nezvimwewo zvinoshandiswa pakubvisa RNA zvese zviri RNase-Mahara.

Yakanatswa nucleic acid inokanganisa kuyedza kwepasi

DNA neRNA zvakacheneswa nekoramu yekuchenesa, kana iyo ion yemunyu uye mapuroteni akanyanya, zvinokanganisa kuyedza kudzika, senge: PCR amplification, reverse transcription, nezvimwe.

1. Iyo yakaraswa DNA neRNA zvine maion emunyu asara.

Zano: Ita shuwa kuti vhoriyamu chaiyo yeabsolute ethanol inowedzerwa kuBuffer RW2, uye geza koramu yekuchenesa kaviri pakumhanya kwecentrifugation inotsanangurwa mumirairo yekushandisa;Ita centrifugation kuderedza kusvibiswa kwemunyu.

2. DNA neRNA yakaraswa ine zvisaririra zve ethanol.

Mazano: Mushure mekusimbisa kushambidza neBuffer RW2, ita isina chinhu tube centrifugation pa centrifugation speed mumirairo yekushanda;kana kuchine ethanol zvakasara, unogona centrifuge chubhu isina chinhu uye woiisa pakamuri tembiricha kwemaminitsi 5 kubvisa ethanol zvakasara kusvika pamwero mukuru.

Mirayiridzo yezvinyorwa:

Viral DNA & RNA Isolation Kit Instruction Manual