Lnc-RT Heroᵀᴹ I(Ne gDNase)(Super Premix yekutanga-strand cDNA synthesis kubva kulncRNA)
Zvinotsanangurwa
25×20μl Rxn, makumi mashanu×20μl Rxns
Lnc-RT Hero TM I (Ne gDNase) is a reverse transcription system yakanyatsogadzirirwa lncRNA kuti ikurumidze kubvisa genomic DNA kusvibiswa.5 × gDNase Mix inogona kukurumidza kubvisa genome yakasara muRNA pa42 ° C kwemaminetsi maviri, inonyatsodzivirira kupindira kwegenome pamigumisiro yeqPCR.
5 × L-RT Hero TM Musanganiswa ine Foregene LncRNA Reverse Transcriptase yakanyatsogadzirwa neForegene, inova rudzi rutsva rwe reverse transcriptase rakanangana nelncRNA uye mamwe marefu eRNA matemplate akaoma, ane yakasimba RNA affinity uye yakakwirira reversal kurekodha kunyatsoita.Iyo yakagadziridzwa sisitimu inoita kuti reverse transcription rate ikurumidze uye inogona kunyora zviri nyore RNA matemplate ane yakakwira GC zvemukati uye yakaoma yechipiri chimiro.Yekutanga strand cDNA synthesis inogona kupedzwa pa42 ° C mumaminitsi gumi nemashanu.
Kit zvikamu
| 5× gDNase Mix |
| 5× L-RT HeroTM Musanganiswa |
| RNase-Yemahara ddH2O |
| Mirayiridzo |
Zvimiro&zvakanakira
■Kugona kugona kubvisa gDNA, iyo inogona kubvisa gDNA mu template mukati me2 maminetsi.
∎ Inokwanisa kudzosera kumashure kunyorwa kwelncRNA.Kana iyo reverse transcript chigadzirwa ichiiswa pasi peqPCR, kukosha kweCt kwakaderera pane kuya kweyakajairwa reverse transcription system.
∎ Sistimu yekunyoreswa kwereverse inoshanda, zvinongotora maminetsi gumi nemashanu kupedzisa kuumbwa kwetambo yekutanga cDNA.
■ Matemplate akaomesesa: matemplate ane yakakwira GC zvemukati uye yakaoma yechipiri chimiro anogona zvakare kudzoserwa kumashure nekunyatsoshanda.
■ High-sensitivity reverse transcription system, pg-level templates inogonawo kuwana yemhando yepamusoro cDNA.
■ Iyo reverse transcription system ine yakanyanya kugadzikana yekupisa, iyo yakakwana yekuita tembiricha i42℃, uye ichine yakanaka reverse transcription performance pa50℃.
Kit application
lncRNA hama yehuwandu hwekuongorora.
lncRNA absolute quantitative analysis.
Inogona kukurumidza uye kunyatsoongorora kutsvaga RNA senge RNA hutachiona.
Reverse transcript yeRNA templates ine yakakwira GC zvemukati kana yakaoma yechipiri chimiro.
Kuyerera kwebasa
Storage uye Sherufu upenyu
Iyo kit inofanira kuchengetwa pa -20°C. Chengetedza chigadzirwa mufiriji yekushisa nguva dzose pa -20°C pakarepo mushure mekugamuchira.Kana mamiriro ekuchengetedza akakodzera, chigadzirwa hachizodzikisi chero mashandiro panguva ye1-gore yechokwadi nguva.
Dambudziko rekuongorora Guide
The following is an analysis of the problems that may be encountered in the use of Lnc-RT HeroTM I (With gDNase) series kits, hoping to be helpful to your experiments. In addition, we have dedicated technical support to help you with other experimental or technical problems beyond the operating instructions and questions. If you have any needs, please contact us: 028-83361257 or E-mail: Tech@foregene.com.
Non-chaiyo amplification
1. Kusanzwisisika primer dhizaini
Kurudziro: Dhizaina maprimers zvinoenderana neprimer dhizaini misimboti.
2. Zvisaririra zveGenome.
Zano: Ita shuwa kuti nhanho yekutanga yekubvisa gDNA reaction tembiricha i42 ° C, uye nguva yekuita inogona zvakare kuwedzerwa kusvika ku5min.
Hapana chiratidzo chekusimudzira neRT-qPCR
1. RNA yakaderedzwa.
Kurudziro: Izvo zvinyorwa zveRNA kudhirowa zvinofanirwa kunge zviri nyowani sezvinobvira, uye yemhando yepamusoro uye yakakwirira-kuchena RNA inofanirwa kushandiswa.
2. RNA ine inhibitors.
Kurudziro: Reverse transcription inhibitors kazhinji inosanganisira SDS, guanidine salts, EDTA, etc. Zvinokurudzirwa kugeza RNA precipitate ne 70% ethanol kubvisa inhibitors.
3. Nyaya dzekutanga dzekugadzira.
Kurudziro: Zvinoenderana neprimer dhizaini musimboti, gadzira patsva maprimers kuti aongororwe.
Iyo bhendi yakanangwa inoonekwa mune isina chinhu control
1. Kusvibiswa kwezvishandiso zvekushandisa kana reagents.
Kurudziro: Zvese ma reagents kana zvishandiso zvekuyedza zvinofanirwa kuve autoclaved.Chenjerera uye unyoro paunenge uchibata kudzivirira DNA samples kubva pakuyamwa mupipette kana kubuda kunze kwechubhu yecentrifuge.
2. Kusvibiswa kwakaitika panguva yekugadzirira kwePCR reaction system.
Kurudziro: Tora matanho anodiwa paunenge uchibata, senge: kupfeka magurovhosi elatex, uchishandisa mafirita matipi.Shandisa Real Time PCR Mix mune inosvibisa-proof system.
3. Maprimers akadzikiswa.
Zvaungaita: Shandisa SDS-PAGE electrophoresis kuona kana maprimers akashatiswa, uye tsiva nemaprimers matsva ekuedza kuongorora fluorescence.
Chinyorwa cheMirayiridzo:
