Fekitari yakapihwa 0.2ml Flat Cap Transparent PCR Tube
Kit Description:
◮A new mouse-derived RNase inhibitor inoratidzwa muE. coli engineered mabhakitiriya achishandisa gene recombination technology.Iyo inhibitor inhibitor RNase chiitiko nekusava nemakwikwi kusanganisa neRNase 1: 1, nekudaro ichidzivirira.RNAkutendeseka, uye inogona kuvharidzira zvinobudiriratheRNase A, B, C chiitiko , asi kwete RNase T1, T2, H, nezvimwewo; Inodzoserwa kumashure kwekusungirirwa kwe RNase Inhibitor uye RNase, uye iyo yakaoma inogona kuparadzaniswa ne urea uye sulfhydryl reagents, kuitira kuti RNase renatures uye inhibitor isingagadziriswi inactivated.
Isu tinovimba nekufunga kwehungwaru, kugara kwechimanjemanje muzvikamu zvese, kufambira mberi kwetekinoroji uyezve kune vashandi vedu vanotora chikamu mukubudirira kwedu kweFekitori yakapihwa 0.2ml Flat Cap Transparent PCR Tube, Isu tinotambira neushamwari kutora kwako kutora chikamu zvinoenderana nekudyidzana kwakawedzerwa mabhenefiti mukati mepedyo nekuuya.
Isu tinovimba nekufunga kwakadzikama, kugara uchivandudzika muzvikamu zvese, kufambira mberi kwetekinoroji uyezve kune vashandi vedu vanotora chikamu mukubudirira kweduChina PCR Tube uye 0.2ml PCR Tube, Mhinduro dzedu dzakanyanya kutengesa kumaodzanyemba-kumabvazuva kweAsia Euro-America, uye kutengesa kunyika yedu yose.Uye zvichienderana nemhando yepamusoro, mutengo unonzwisisika, sevhisi yakanakisa, isu tine mhinduro yakanaka kubva kune vatengi mhiri kwemakungwa.Unogamuchirwa kuti ubatane nesu kune mamwe mikana uye mabhenefiti.Isu tinogashira vatengi, masangano emabhizinesi uye shamwari kubva kumativi ese epasi kuti tibate isu uye kutsvaga kushandira pamwe kuti zvibatsire.
Zvinotsanangurwa
5ml, 10ml, 40ml
PCR Gamba (NeDye) Yekuwedzera kit inosanganisira yeForegene yakasarudzika chizvarwa chitsva cheTaq-Foregene Taq Gamba, yakagadziridzwa dNTPs, MgCl2, reaction buffer, PCR reaction inosimudzira, optimizer uye stabilizer.Iyo 2 × PCR Hero TM Musanganiswa (dhayi) system ine kushivirira kwakanyanya kune PCR inhibitors kupfuura yakajairwa PCR Mix system, uye inogona kutarisana nyore nePCR kukwidziridzwa kweakasiyana matemplate akaoma.Iyo yakasarudzika maitiro sisitimu uye yakakwirira-inoshanda Taq Gamba inoita kuti PCR iite ive nepamusoro amplification kunyatsoita, kujeka uye kunzwa.
Kit zvikamu
| 0.1U Taq Gamba/μl |
| 400μM dNTPs |
| 20mM Tris-HCl(pH8.8) |
| 100mM KCl |
| 5mM MgCl2 |
| Zvimwe zvinosimbisa uye zvinowedzera |
| DNA Loading Buffer |
Zvimiro&zvakanakira
■ Kuvimbika kwepamusoro: kakapetwa ka6 kuya kweye enzyme yeTaqwo zvayo;
■ Inokurumidza amplification sipidhi : sipidhi yeamplification ndeye 5-10sec/kb, inova ka6-12 kakapetwa ka12 kune yakajairika Taq enzyme;
■ Kuwedzera kuchinjika kwetemplate: Inogona kukudza zvakasiyana-siyana zvakaoma matemplate eDNA nepamusoroGC zvemukati;
∎ Kukwirisa kwepamusoro: mitsetse yekusimudzira yakaderera pane yakajairika Taq enzyme;
■ Kushivirira kwezvakatipoteredza kwakasimba: kuiswa pa 37 ° C kwevhiki, kuchengetedza zvinopfuura 90% basa;
■ Iine 5′ →3′ DNA polymerase basa uye 5′→3′ exonuclease basa, pasina 3′ →5′ exonuclease basa.
■ Iyo kititi yakagara yakasanganiswa neElectrophoresis Loading Buffer, mushure mokunge PCR yapera, sampu inogona kutakurwa zvakananga kune electrophoresis;kushanda kuri nyore uye nyore.
Kit application
PCR kukwidziridzwa kwezvimedu zveDNA, kunyora DNA, DNA sequencing,PCR pamwe nemuswe
Storage uye Sherufu upenyu
Inogona kuchengetwa kwenguva yakareba pa -20 ° C;kuti ishandiswe nguva dzose, inogona kuchengetwa pa4 ° C kwenguva pfupi. Isu tinovimba nekufunga kwakarongeka, nguva dzose yemazuva ano muzvikamu zvose, kufambira mberi kwetekinoroji uye hongu pane vashandi vedu vanotora chikamu mukubudirira kwedu kweFactory yakapiwa 0.2ml Flat Cap Transparent PCR Tube, Tinokugamuchira noushamwari kutora chikamu chako kunoenderana nepedyo kune mamwe mabhenefiti ari kuuya.
Factory inopihwaChina PCR Tube uye 0.2ml PCR Tube, Mhinduro dzedu dzakanyanya kutengesa kumaodzanyemba-kumabvazuva kweAsia Euro-America, uye kutengesa kunyika yedu yose.Uye zvichienderana nemhando yepamusoro, mutengo unonzwisisika, sevhisi yakanakisa, isu tine mhinduro yakanaka kubva kune vatengi mhiri kwemakungwa.Unogamuchirwa kuti ubatane nesu kune mamwe mikana uye mabhenefiti.Isu tinogashira vatengi, masangano emabhizinesi uye shamwari kubva kumativi ese epasi kuti tibate isu uye kutsvaga kushandira pamwe kuti zvibatsire.
