Isu tinosimbisa kusimudzira uye kuunza mhinduro nyowani mumusika munenge gore rega rega reFactory sosi Yakakwirira Kuvimbika Hotstart Taq Mix PCR Kit PCR Master Mix 2 × A8 Fasthifi PCR Mastermix, Takazvipira kupa hunyanzvi hwekunatsa tekinoroji uye sarudzo dzako iwe pachako!
Isu tinosimbisa kusimudzira uye kuunza mhinduro nyowani mumusika ingangoita gore rega regaChina PCR Kit uye PCR, Kusvika parizvino, runyorwa rwezvinhu rwakagadziridzwa nguva nenguva uye rwunokwezva vatengi kubva kutenderera pasirese.Zvakadzama chokwadi chinowanzo kuwanikwa muwebhu-saiti yedu uye iwe unozoshandirwa neprimiyamu yemhando yezano sevhisi neboka redu rekutengesa.Ivo vachakubatsira iwe kuwana zvakadzama kubvuma nezvezvinhu zvedu uye kuita kugutsikana kutaurirana.Kambani kuenda kufekitari yedu muBrazil inogamuchirwawo chero nguva.Ndinovimba kuwana kubvunza kwako kune chero inofara co-operation.
Chinyorwa cheMirayiridzo:

Isu tinosimbisa kusimudzira uye kuunza mhinduro nyowani mumusika munenge gore rega rega reFactory sosi Yakakwirira Kuvimbika Hotstart Taq Mix PCR Kit PCR Master Mix 2 × A8 Fasthifi PCR Mastermix, Takazvipira kupa hunyanzvi hwekunatsa tekinoroji uye sarudzo dzako iwe pachako!
Factory sourceChina PCR Kit uye PCR, Kusvika parizvino, runyorwa rwezvinhu rwakagadziridzwa nguva nenguva uye rwunokwezva vatengi kubva kutenderera pasirese.Zvakadzama chokwadi chinowanzo kuwanikwa muwebhu-saiti yedu uye iwe unozoshandirwa neprimiyamu yemhando yezano sevhisi neboka redu rekutengesa.Ivo vachakubatsira iwe kuwana zvakadzama kubvuma nezvezvinhu zvedu uye kuita kugutsikana kutaurirana.Kambani kuenda kufekitari yedu muBrazil inogamuchirwawo chero nguva.Ndinovimba kuwana kubvunza kwako kune chero inofara co-operation.

Dambudziko rekuongorora Guide

The following is an analysis of the problems that may be encountered in the extraction of plant genomic DNA, hoping to be helpful to your experiments. In addition, for other experimental or technical problems other than operation instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us: 028-83360257 or E-mali: Tech@foregene.com.

Goho rakaderera kana pasina DNA

Kazhinji pane zvinhu zvakawanda zvinokanganisa goho rejenomic DNA, kusanganisira kunobva sampuli, zera remuenzaniso, mamiriro ekuchengetedza emuenzaniso, uye kushanda.

Genomic DNA haina kukwanisa kuwanikwa panguva yekubvisa

1. Matisue samples anochengetwa zvisina kunaka kana kuchengetwa kwenguva yakareba, zvichikonzera kuparara kwegenomic DNA.

Kurudziro: Chengeta masamples ematishu mune mvura nitrogen kana -20°C;edza kushandisa zvichangobva kuunganidzwa sampuli dzegenomic DNA kutorwa.

2. Yakanyanya kudikisa sampuli yemari inogona kukonzera inoenderana genomic DNA kuti isaburitswe.

Zano: Kumasampuli ematishu akachengetwa kwenguva yakareba kana ane yakanyanya genomic DNA degradation, huwandu hwemasampure ematissue hunogona kuwedzerwa nenzira kwayo kuitira kuburitsa yakawanda genomic DNA.Nhamba yemuenzaniso inogona kugadziriswa maererano nezvinodiwa zveDNA, asi mutsva mutsva haufaniri kudarika 100mg, uye sampuli yakaoma haifaniri kudarika 30mg.

3. Muenzaniso hausi pasi nenitrogen yemvura kana kuiswa kwenguva yakareba mushure memvura yenitrogen.

Zano: Panguva yekutorwa kweDNA, sampuli inoda kuve yakazara pasi nenitrogen yemvura kuti iparadze rusvingo rwesero;mushure mekugaya, ndapota tumira sampuli yehupfu kuPL1 preheated pa65°C nokukurumidza sezvinobvira (kana pasi poda yakanyunguduka, genomic DNA inotanga kuderera nokukurumidza) .

4. Kuchengetwa kusina kunaka kweForegene Protease kunoguma nekuderedzwa kana kusashanda basa.

Kurudziro: Simbisa mamiriro ekuchengetedza eForegene Protease kana kuitsiva neForegene Protease itsva ye enzymatic hydrolysis.

5. Iyo kiti inochengetwa zvisina kunaka kana kuchengetwa kwenguva yakareba, zvichiita kuti zvimwe zvikamu mukiti zvikundike.

Kurudziro: Tenga chirimwa chitsva genomic DNA yekubvisa kit yemabasa ane hukama.

6. Kushandiswa kusina kunaka kwekiti.

Zano: Tenga Plant DNA Isolation Kit yakatsaurirwa kune masampuli ekubvisa uye kucheneswa kwechirimwa genomic DNA.

7. Buffer WB pasina kuwedzera anhydrous ethanol.

Kurudziro: Ita shuwa kuti wawedzera iyo chaiyo vhoriyamu yeabsolute ethanol kuBuffer WB.

8. Eluent haina kudonhedzwa pa silica membrane nemazvo.

Zvaungaita: Wedzera pre-heated eluent pa65℃inodonha kusvika pakati pe silica gel membrane, uye woisiya iri pakamuri tembiricha kwemaminetsi mashanu kuti uwedzere kujekesa.

Kutora kuti uwane yakaderera-goho genomic DNA

1. Sample inochengetwa zvisina kunaka kana kuchengetwa kwenguva yakareba, zvichikonzera kuparara kwegenomic DNA.

Kurudziro: Chengetedza matishu samples pa -20℃;edza kushandisa zvichangobva kuunganidzwa matishu samples kuti genomic DNA kutorwa.

2. Kana huwandu hwemasikirwo emasikirwo ari mashoma, iyo yakabviswa genomic DNA ichava shoma.

Zano: Mamwe masampuli ezvirimwa akapfuma mumvura, senge zvirimwa zvemumvura zvakaita sealgae, zvichingodaro, dosi inogona kuwedzerwa zvakakodzera kana kuti mvura inogona kupera mvura shoma isati yaitwa.

3. Mienzaniso yakanga isina kunyatsogadzika nenitrogen yemvura kana kuti yakasiiwa patembiricha yekamuri kwenguva yakareba mushure mekukuya.

Zano: Iyo nitrogen yemvura inokuya inofanira kukwana, uye sampuli sero wall inofanira kuputswa zvakanyanya sezvinobvira;pakarepo mushure mekukuya, sampuli yehupfu inofanira kuendeswa ku65℃preheated Buffer PL1 yedanho rinotevera.

4. Kusashandisa kit chaiyo.

Kurudziro: Shandisa yakazvipira Plant DNA Isolation Kit kuburitsa uye kuchenesa chirimwa genomic DNA.

5. Kuchengetedzwa kwakashata kweForegene Protease kunoguma nekuderedzwa kana kusashanda basa.

Kurudziro: Simbisa mamiriro ekuchengetedza eForegene Protease kana kuitsiva neForegene Protease itsva ye enzymatic hydrolysis.

6. Eluent dambudziko

Kurudziro: Ndapota shandisa Buffer EB ye elution;kana uchishandisa ddH₂O kana zvimwewo zvinonzwisiswa, ita shuwa kuti pH yeiyo eluent iri pakati pe7.0-8.5.

7. Rukudzo haruna kudonha nemazvo

Zano: Ndokumbirawo uwedzere kudonhedza kudonhedza pakati pe silica membrane uye woisiya iri pakamuri tembiricha kwemaminetsi mashanu kuti uwedzere kujekesa.

8. Vhoriyamu yakawandisa idiki

Zvaungaita: Ndokumbira ushandise iyo eluent ye genomic DNA elution zvinoenderana nemirairo, ingangoita isingasviki zana.μl.

Yakabviswa genomic DNA ine kuchena kwakaderera

Iyo yakaderera kuchena kweiyo genomic DNA ichaita kuti kukundikana kana kushata kwemaitiro ezasi ekuedza, senge: iyo enzyme haigone kutemwa, uye chinangwa chegene fragment hachigone kuwanikwa nePCR.

1. Miscellaneous protein kusvibiswa, RNA kusvibiswa.

Kuongorora: Buffer PW haina kushandiswa kushambidza mbiru;Buffer PW haina kushandiswa kushambidza mbiru pane chaiyo centrifugation kumhanya.

Zvaungaita: edza kuona kuti hapana kunaya kwemvura musimba repamusoro apo simba repamusoro rinopfuura nepakati;iva nechokwadi chekugeza koramu yekuchenesa neBuffer PW maererano nemirairo, uye danho iri harigoni kusiiwa.

2. Kusvibiswa ion kusvibiswa.

Ongororo: Iyo Buffer WB wash column yakasiiwa kana kungogezwa kamwe chete, zvichikonzera kusvibiswa kweionic kwasara.

Kurudziro: Iva nechokwadi chekugeza kaviri neBuffer WB maererano nemirairo yekubvisa maion akasara zvakanyanya sezvinobvira.

3. RNase kusvibiswa.

Kuongorora: Exogenous RNase inowedzerwa kuBuffer;Kushambidzika kwakashata muBuffer PW kunozokonzera RNase yakasara uye kukanganisa maekisesheni ekudzika kweRNA, senge in vitro transcription.

Zano: Foregene series nucleic acid extraction kits inogona kubvisa RNA pasina imwe RNase, uye mareagents ese muPlant DNA Isolation Kit haadi RNase;iva nechokwadi chekugeza koramu yekuchenesa neBuffer PW maererano nemirairo, uye danho iri harigoni kusiiwa.

4. Ethanol zvisaririra.

Ongororo: Mushure mekugeza koramu yekuchenesa neBuffer WB, hapana isina chinhu chubhu centrifugation yakaitwa.

Kurudziro: Tevedzera mirairo yekwakakodzera isina chinhu chubhu centrifugation.

Chinyorwa cheMirayiridzo:

Plant DNA Isolation Kit Instruction Manual