Tora basa rakazara rekugutsa zvese zvinodiwa nevatengi vedu;kusvika kubudiriro dzakadzikama nekushambadzira kusimudzira kwevatengi vedu;kukura kuve wekupedzisira anogara akabatana mubatsiri wevatengi uye nekuwedzera zvinofarirwa nevatengi veFactory sosi China Rt PCR Assay Reagent ye2019 New Virus (ORF1ab, N gene), Isu tinozvisimbisa pachedu kugadzira kubudirira kunoshamisa tichiri kugona.Isu tanga tichitsvaga kuve mumwe wevatengesi vako vakavimbika.
Tora basa rakazara rekugutsa zvese zvinodiwa nevatengi vedu;kusvika kubudiriro dzakadzikama nekushambadzira kusimudzira kwevatengi vedu;kukura kuva wekupedzisira anogara akabatana mubatsiri wevatengi uye kuwedzera zvinofarirwa nevatengiChina PCR Kit, Pcr Detection Kit, Tichitarisa mberi, ticharamba tichifambirana nenguva, tichienderera mberi nekugadzira zvigadzirwa zvitsva.Nechikwata chedu chekutsvaga chakasimba, zvivakwa zvekugadzira zvepamberi, manejimendi esainzi uye masevhisi epamusoro, tichapa zvigadzirwa zvemhando yepamusoro kune vatengi vedu pasi rese.Isu tinokukokai nemoyo wese kuti muve vadyidzani vedu vebhizinesi kuitira kubatsirana.

Tsanangudzo

Iyi kit inoshandisa yakasarudzika lysis buffer sisitimu inogona kukurumidza kuburitsa RNA kubva kune yakasimwa maseru masampula eRT-qPCR maitiro, nekudaro ichibvisa iyo inopedza nguva uye yakaoma RNA yekuchenesa maitiro.Iyo RNA template inogona kuwanikwa mumaminetsi manomwe chete.Iyo 5 × Direct RT Musanganiswa uye 2 × Direct qPCR Musanganiswa-SYBR reagents anopihwa nekiti anogona nekukurumidza uye zvinobudirira kuwana chaiyo-nguva yehuwandu hwePCR mhinduro.

5 × Yakananga RT Mix uye 2 × Yakananga qPCR Mix-SYBR ine inhibitor kushivirira kwakasimba, uye lysate yemasampuli inogona kushandiswa se template yeRT-qPCR zvakananga.Iyi kit ine yakasarudzika RNA yakakwirira-yakabatana Foregene reverse transcriptase, uye Inopisa D-Taq DNA polymerase, dNTPs, MgCl.₂, reaction buffer, PCR optimizer uye stabilizer.

Zvinotsanangurwa

200×20μl Rxns, 1000×20μl Rxns

Kit zvikamu

Zvimiro&zvakanakira

■ Zviri nyore uye zvinoshanda : neTekinoroji yeCell Direct RT, masampuli eRNA anogona kuwanikwa mumaminetsi manomwe chete.

∎ Sample kudiwa idiki, yakaderera semaseru gumi anogona kuongororwa.

■ High throughput: inogona kukurumidza kuona RNA mumaseru akagadzirwa mu384, 96, 24, 12, 6-tsime mahwendefa.

∎ DNA Eraser inogona kukurumidza kubvisa majenomes akaburitswa, kuderedza zvakanyanya kukanganiswa pamhedzisiro yekuedza.

■ Optimized RT uye qPCR sisitimu inoita kuti nhanho mbiri RT-PCR reverse transcription inyatsoita uye PCR yakanyatsojeka, uye inoshingirira kuRT-qPCR reaction inhibitors.

Kit application

Kuwanda kwekushandiswa: masero akagadzirwa.

- RNA yakaburitswa nesample lysis: inoshanda chete kune RT-qPCR template yeiyi kit.

- Iyo kit inogona kushandiswa kune zvinotevera zvinangwa: gene kutaura kuongororwa, kuoneswa kweSiRNA-mediated gene kunyaradza maitiro, kuongorora zvinodhaka, nezvimwe.

Mufananidzo

Storage uye Sherufu upenyu

Chikamu I chekiti ichi chinofanira kuchengetwa pa4 ℃;Chikamu II chinofanira kuchengetwa pa -20 ℃.

Foregene Protease Plus II inofanira kuchengetwa pa 4 ℃, isaome pa -20 ℃.

Reagent 2 × Direct qPCR Mix-SYBR inofanira kuchengetwa pa -20 ℃ murima;kana yakashandiswa kakawanda, inogonawo kuchengetwa pa4 ℃ yekuchengetedza kwenguva pfupi (shandisa mukati memazuva gumi) .Kutora basa rakazara kugutsa zvose zvinodiwa nevatengi vedu;kusvika kubudiriro dzakadzikama nekushambadzira kusimudzira kwevatengi vedu;kukura kuve wekupedzisira anogara akabatana mubatsiri wevatengi uye nekuwedzera zvinofarirwa nevatengi veFactory sosi China Rt PCR Assay Reagent ye2019 New Virus (ORF1ab, N gene), Isu tinozvisimbisa pachedu kugadzira kubudirira kunoshamisa tichiri kugona.Isu tanga tichitsvaga kuve mumwe wevatengesi vako vakavimbika.
Factory sourceChina PCR Kit, PCR Detection Kit, Tichitarisa mberi, ticharamba tichifambirana nenguva, tichienderera mberi nekugadzira zvigadzirwa zvitsva.Nechikwata chedu chekutsvaga chakasimba, zvivakwa zvekugadzira zvepamberi, manejimendi esainzi uye masevhisi epamusoro, tichapa zvigadzirwa zvemhando yepamusoro kune vatengi vedu pasi rese.Isu tinokukokai nemoyo wese kuti muve vadyidzani vedu vebhizinesi kuitira kubatsirana.

Real Time PCR primer dhizaini misimboti

Pamberi Primer uye Reverse Primer

Kune Real Time PCR, dhizaini yekutanga yakakosha.MaPrimers ane hukama nehunyanzvi uye kugona kwePCR amplification, uye inogona kugadzirwa ichitarisa kune anotevera misimboti:

Kureba kwekutanga: 18-30bp.

GC yemukati: 40-60%.

Tm kukosha: Primer dhizaini software, senge Primer 5, inogona kupa iyo Tm kukosha kweiyo primer.Iyo Tm tsika dzekumusoro uye pasi pemvura dzekutanga dzinofanira kunge dziri padyo sezvinobvira.The Tm calculation formula inogonawo kushandiswa: Tm = 4 °C (G + C) + 2 °C (A + T).Paunenge uchiita PCR, tembiricha iri pasi peiyo primer Tm kukosha kwe5 ° C inowanzo sarudzwa seye tembiricha yekudziya (iyo inowirirana kuwedzera kwekushisa kweanona inogona kuwedzera iyo chaiyo yePCR reaction).

Primers uye PCR zvigadzirwa:

Dhizaini primer PCR yekusimudzira chigadzirwa kureba ingangoita 100-150bp.

Dhizaini yekutanga munzvimbo yechipiri yemaitiro eiyo template inofanira kudziviswa zvakanyanya sezvinobvira.

Dzivisa kuumbwa kwe 2 kana anopfuura mabhesi anowirirana pakati pe 3′ migumo yekumusoro nekudzika kwemvura.

Primer 3′ terminal base haigone kuvepo ne3 yekuwedzera yakatevedzana G kana C.

Iwo maprimer pachawo haagone kuve neanopindirana zvimiro, zvikasadaro chimiro chebvudzi chinoumbwa, chinokanganisa PCR amplification.

ATCG inofanirwa kugovaniswa zvakaenzana sezvinobvira mukutevedzana kwekutanga, uye 3 ′ terminal base inofanira kudzivirirwa seT.

Appendikisi 1: Cell Direct RT-qPCR Kit chikamu chekuwedzera paki

1.Sero Lysis Solution