Isu tinotenda kuti kudyidzana kwenguva refu ndeye mhedzisiro yemhando yepamusoro, yakakosha masevhisi, ruzivo rwakabudirira uye kuonana nemunhu fekitori Zviteshi zveVirus Magnetic Bead Method Rna-DNA Nucleic Acid 96 Sample Extraction Purification Tube Kit, Isu tinonzwa kuti vane shungu, vanopwanya uye vakanyatsodzidziswa vashandi vanogona kugadzira inonakidza uye inobatsira hukama hwebhizinesi nekukurumidza.Iva nechokwadi chekunyatsonzwa wakasununguka kutibata isu kuti uwane rumwe ruzivo.
Isu tinotenda kuti kudyidzana kwenguva refu ndeye mhedzisiro yemhando yepamusoro, yakakosha masevhisi akawedzerwa, ruzivo rwakabudirira uye kuonana nemunhu wegaChina Nucleic Acid Extraction Kit (rna-DNA) uye 96 Nucleic Acid, Tichisimbirira pane yemhando yepamusoro chizvarwa manejimendi uye vatengi pro rubatsiro, isu zvino takagadzira sarudzo yedu kupa vatengi vedu vachishandisa iyo kutanga nehuwandu hwekuwana uye mushure mesevhisi ruzivo runoshanda.Kuchengetedza hukama huripo hwehushamwari nevatengi vedu, isu zvakadaro tinogadzira mhinduro yedu inonyora nguva dzese kugutsa izvo zvinodikanwa zvitsva uye kuomerera kune yazvino-yezvino budiriro yemusika muMalta.Isu takagadzirira kutarisana nekunetseka uye kuita kuvandudza kuti tinzwisise zvese zvingangoitika mukutengeserana kwenyika.

Zvinotsanangurwa

50 Preps, ye genomic DNA kucheneswa kubva muropa rose uchishandisa <1ml ropa

Buffer DC: Inoisa masero matsvuku eropa muropa.

Buffer BL1: Ipa muenzaniso enzymatic digestion nharaunda.

Foregene Protease Plus: Enzymatic digest masampu eropa munzvimbo yeBuffer BL1.

Buffer BL2: Ita kuti Foregene Protease Plus uye ipe DNA yekurodha nharaunda.

Buffer PW: Bvisa tsvina yakaita seprotein uye RNA muDNA.

Buffer WB1: Bvisa maion emunyu asara muDNA.

Buffer EB: Elute iyo DNA pane yekuchenesa column membrane.

DNA-Chete Column: Kunyanya adsorbs genomic DNA mune lysate.

Kit zvikamu

Zvimiro&zvakanakira

-Kwete kusvibiswa kweRNase: Iyo DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva kune genomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.

-Kukurumidza kukurumidza: Foregene Protease Plus ine basa rakakwirira kudarika maproteases akafanana, uye inochera sampuli dzenyama nokukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me40 maminetsi.

-Kusununguka: Iyo centrifugation inoitwa pakupisa kwekamuri, 4 ° C centrifugation kana ethanol precipitation yeDNA haidiwi.

-Kuchengeteka: Hapana organic reagent kutorwa kunoshandiswa.

-Hunhu hwepamusoro: Iyo yakabviswa genomic DNA zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, iyo inogona kusangana nezvinodiwa zvekuedza kwakasiyana.

Kit application

Inokodzera kubviswa uye kucheneswa kwegenomic DNA kubva kutsva kana chando anticoagulated ropa rose.

Workflow

Storage uye Sherufu upenyu

Iyi kit inogona kuchengetwa kwemwedzi gumi nemiviri pasi pemamiriro akaoma panzvimbo yekupisa (15-25 ° C);kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa pa2-8°C.

Ongorora: Kana yakachengetwa pane tembiricha yakaderera, mhinduro yacho inonangana nekunaya.Usati washandisa, iva nechokwadi chekuisa mhinduro mukiti pane tembiricha yekamuri kwenguva yakati.Kana zvichidikanwa, preheat mubhati yemvura ye 37 ° C kwemaminitsi gumi kuti iparadze mvura, uye isanganise isati yashandiswa.

Iyo Foregene Protease Plus mhinduro ine yakasarudzika formula, inoshanda kana yakachengetwa mukamuri tembiricha kwenguva yakareba (3 mwedzi);ichange ichinyanya kushanda uye yakagadzikana kana yakachengetwa pa4 ° C, saka inokurudzirwa kuichengeta pa 4 ° C, yeuka kuti usaichengeta pa -20 ° C. Tinotenda kuti hukama hurefu hwekutaura huri mugumisiro wehutano hwepamusoro, hunokosha hunoita mabasa, ruzivo rwakabudirira uye kuonana kwega kwefekitari Zvigadzirwa zveVirus Magnetic Bead Method Rna-DNA Nucleic Acid , Kuchenesa zvakanaka Kuchenesa uye Kudzidzira Kufukura kweSampuli 96 basa rinoputika uye Kuchenesa Sample simba rinogona kugadzira hukama hwakanaka uye hunobatsira bhizinesi newe nekukurumidza.Iva nechokwadi chekunyatsonzwa wakasununguka kutibata isu kuti uwane rumwe ruzivo.
factory Zvitengesi zveChina Nucleic Acid Extraction Kit (rna-DNA) uye 96 Nucleic Acid, Tichisimbirira pane yemhando yepamusoro chizvarwa manejimendi uye vatengi pro rubatsiro, isu zvino takagadzira sarudzo yedu kupa vatengi vedu vachishandisa iyo kutanga nehuwandu hwekuwana uye mushure mesevhisi ruzivo runoshanda.Kuchengetedza hukama huripo hwehushamwari nevatengi vedu, isu zvakadaro tinogadzira mhinduro yedu inonyora nguva dzese kugutsa izvo zvinodikanwa zvitsva uye kuomerera kune yazvino-yezvino budiriro yemusika muMalta.Isu takagadzirira kutarisana nekunetseka uye kuita kuvandudza kuti tinzwisise zvese zvingangoitika mukutengeserana kwenyika.