Sangano rinoramba richifunga nezvemaitiro ekuti "Sainzi manejimendi, hukuru hwepamusoro uye hukuru hwekuita, mutengi mukuru weChina Mugadziri we2 × Yakagadziriswa Premix yeUsina Kucheneswa Sample Real-Nguva Quantitative PCR Direct Multiplex Probe Qpcr Mix Plus U, Bhizinesi redu rakazvipira kupa vatengi zvakakosha uye zvakachengeteka zvemhando yepamusoro zvigadzirwa nemutengo wega wega uye zvine hukasha.
Sangano rinoramba riri padanho rekuti "Sainzi manejimendi, mhando yepamusoro uye hukuru hwekuita, mutengi mukuruChina Taq DNA Polymerase uye Qpcr, Kambani yedu ine simba rakawanda uye ine yakatsiga uye yakakwana yekutengesa network system.Tinoshuva kuti isu taikwanisa kumisa hukama hwebhizinesi hwakanaka nevatengi vese kubva kumba nekune dzimwe nyika pahwaro hwekubatsirikana.
Real Time PCR primer dhizaini misimboti

Pamberi Primer uye Reverse Primer

Kune Real Time PCR, dhizaini yekutanga yakakosha.MaPrimers ane hukama nehunyanzvi uye kugona kwePCR amplification, uye inogona kugadzirwa ichitarisa kune anotevera misimboti:

• Kureba kwekutanga: 18-30bp.
• GC yemukati: 40-60%.
• Tm kukosha: Primer dhizaini software, senge Primer 5, inogona kupa iyo Tm kukosha kweiyo primer.Iyo Tm tsika dzekumusoro uye pasi pemvura dzekutanga dzinofanira kunge dziri padyo sezvinobvira.The Tm calculation formula inogonawo kushandiswa: Tm = 4 °C (G + C) + 2 °C (A + T).Paunenge uchiita PCR, tembiricha iri pasi peiyo primer Tm kukosha kwe5 ° C inowanzo sarudzwa seye tembiricha yekudziya (iyo inowirirana kuwedzera kwekushisa kweanona inogona kuwedzera iyo chaiyo yePCR reaction).
• Primers uye PCR zvigadzirwa:

1. Dhizaini primer PCR yekusimudzira chigadzirwa kureba ingangoita 100-150bp.
2. Dhizaini yekutanga munzvimbo yechipiri yemaitiro eiyo template inofanira kudziviswa zvakanyanya sezvinobvira.
3. Dzivisa kuumbwa kwe 2 kana anopfuura mabhesi anowirirana pakati pe 3′ migumo yekumusoro nekudzika kwemvura.
4. Primer 3′ terminal base haigone kuvepo ne3 yekuwedzera yakatevedzana G kana C.
5. Iwo maprimer pachawo haagone kuve neanopindirana zvimiro, zvikasadaro chimiro chebvudzi chinoumbwa, chinokanganisa PCR amplification.
6. ATCG inofanirwa kugovaniswa zvakaenzana sezvinobvira mukutevedzana kwekutanga, uye 3 ′ terminal base inofanira kudzivirirwa seT.

Appendikisi1:Cuye DirectRT-qPCR Kit component supplement pack

1.Cell Lysis Solution

 Sangano rinoramba richifunga nezvemaitiro ekuti "Sainzi manejimendi, hukuru hwepamusoro uye hukuru hwekuita, mutengi mukuru weChina Mugadziri we2 × Yakagadziriswa Premix yeUsina Kucheneswa Sample Real-Nguva Quantitative PCR Direct Multiplex Probe Qpcr Mix Plus U, Bhizinesi redu rakazvipira kupa vatengi zvakakosha uye zvakachengeteka zvemhando yepamusoro zvigadzirwa nemutengo wega wega uye zvine hukasha.
China Mugadziri weChina Taq DNA Polymerase uye Qpcr, Kambani yedu ine simba rakawanda uye ine yakatsiga uye yakakwana yekutengesa network system.Tinoshuva kuti isu taikwanisa kumisa hukama hwebhizinesi hwakanaka nevatengi vese kubva kumba nekune dzimwe nyika pahwaro hwekubatsirikana.

Real Time PCR primer dhizaini misimboti

Pamberi Primer uye Reverse Primer

Kune Real Time PCR, dhizaini yekutanga yakakosha.MaPrimers ane hukama nehunyanzvi uye kugona kwePCR amplification, uye inogona kugadzirwa ichitarisa kune anotevera misimboti:

• Kureba kwekutanga: 18-30bp.
• GC yemukati: 40-60%.
• Tm kukosha: Primer dhizaini software, senge Primer 5, inogona kupa iyo Tm kukosha kweiyo primer.Iyo Tm tsika dzekumusoro uye pasi pemvura dzekutanga dzinofanira kunge dziri padyo sezvinobvira.The Tm calculation formula inogonawo kushandiswa: Tm = 4 °C (G + C) + 2 °C (A + T).Paunenge uchiita PCR, tembiricha iri pasi peiyo primer Tm kukosha kwe5 ° C inowanzo sarudzwa seye tembiricha yekudziya (iyo inowirirana kuwedzera kwekushisa kweanona inogona kuwedzera iyo chaiyo yePCR reaction).
• Primers uye PCR zvigadzirwa:

1. Dhizaini primer PCR yekusimudzira chigadzirwa kureba ingangoita 100-150bp.
2. Dhizaini yekutanga munzvimbo yechipiri yemaitiro eiyo template inofanira kudziviswa zvakanyanya sezvinobvira.
3. Dzivisa kuumbwa kwe 2 kana anopfuura mabhesi anowirirana pakati pe 3′ migumo yekumusoro nekudzika kwemvura.
4. Primer 3′ terminal base haigone kuvepo ne3 yekuwedzera yakatevedzana G kana C.
5. Iwo maprimer pachawo haagone kuve neanopindirana zvimiro, zvikasadaro chimiro chebvudzi chinoumbwa, chinokanganisa PCR amplification.
6. ATCG inofanirwa kugovaniswa zvakaenzana sezvinobvira mukutevedzana kwekutanga, uye 3 ′ terminal base inofanira kudzivirirwa seT.

Appendikisi1:Cuye DirectRT-qPCR Kit component supplement pack

1.Cell Lysis Solution

Mirayiridzo yezvinyorwa:

 Kurumidza Nyore Sero Yakananga RT-qPCR Kit-Taqman