Mutengo Wakanyanya weTaq DNA Polymerase
Kit Description:
Foreasy M-MLV Reverse Transcriptase is a new reverse transcriptase inoratidzwa muE. coli mabhakitiriya akagadzirwa achishandisa genetic recombination technology.Iyo inodzokorodza DNA polymerase iyo inogadzira inowirirana DNA strand kubva kune imwe-stranded RNA, DNA, kana RNA: DNA hybrid.Iyo haina RNase H chiitiko, kugadzikana kwakasimba, yakasimba RNA affinity, uye yakakwirira yekuona senitivity.
Tichifunga nezve motto iyi, tave vamwe vevagadziri vehunyanzvi hwekuita tekinoroji, vanodhura, uye vanokwikwidza mutengo weMutengo Wakanakisa weTaq DNA Polymerase, Kuti tiwane mabhenefiti anoenderana, kambani yedu iri kuwedzera zvakanyanya maitiro edu ekudyidzana kwepasirese maererano nekutaurirana nevatengi vekunze, kuendesa nekukurumidza, kunaka kwemhando yepamusoro uye kubatana kwenguva refu.
Tichifunga nezve motto iyi, tave imwe yevanonyanya kugadzira tekinoroji, inodhura-inoshanda, uye inokwikwidza mutengo vagadziriChina 2X Taq Mstermix uye Mmlv Taq, Kambani yedu yakagara ichisimbirira pamusimboti webhizinesi we "Hunhu, Kutendeseka, uye Mutengi Kutanga" iyo yatakawana nayo kuvimba nevatengi kubva kumba nekune dzimwe nyika.Kana iwe uchifarira zvekutengesa kwedu, haufanirwe kuzeza kutibata kuti uwane rumwe ruzivo.
Zvinotsanangurwa
5ml, 10ml, 40ml
PCR Gamba (NeDye) Yekuwedzera kit inosanganisira yeForegene yakasarudzika chizvarwa chitsva cheTaq-Foregene Taq Gamba, yakagadziridzwa dNTPs, MgCl2, reaction buffer, PCR reaction inosimudzira, optimizer uye stabilizer.Iyo 2 × PCR Hero TM Musanganiswa (dhayi) system ine kushivirira kwakanyanya kune PCR inhibitors kupfuura yakajairwa PCR Mix system, uye inogona kutarisana nyore nePCR kukwidziridzwa kweakasiyana matemplate akaoma.Iyo yakasarudzika maitiro sisitimu uye yakakwirira-inoshanda Taq Gamba inoita kuti PCR iite ive nepamusoro amplification kunyatsoita, kujeka uye kunzwa.
Kit zvikamu
| 0.1U Taq Gamba/μl |
| 400μM dNTPs |
| 20mM Tris-HCl(pH8.8) |
| 100mM KCl |
| 5mM MgCl2 |
| Zvimwe zvinosimbisa uye zvinowedzera |
| DNA Loading Buffer |
Zvimiro&zvakanakira
■ Kuvimbika kwepamusoro: kakapetwa ka6 kuya kweye enzyme yeTaqwo zvayo;
■ Inokurumidza amplification sipidhi : sipidhi yeamplification ndeye 5-10sec/kb, inova ka6-12 kakapetwa ka12 kune yakajairika Taq enzyme;
■ Kuwedzera kuchinjika kwetemplate: Inogona kukudza zvakasiyana-siyana zvakaoma matemplate eDNA nepamusoroGC zvemukati;
∎ Kukwirisa kwepamusoro: mitsetse yekusimudzira yakaderera pane yakajairika Taq enzyme;
■ Kushivirira kwezvakatipoteredza kwakasimba: kuiswa pa 37 ° C kwevhiki, kuchengetedza zvinopfuura 90% basa;
■ Iine 5′ →3′ DNA polymerase basa uye 5′→3′ exonuclease basa, pasina 3′ →5′ exonuclease basa.
■ Iyo kititi yakagara yakasanganiswa neElectrophoresis Loading Buffer, mushure mokunge PCR yapera, sampu inogona kutakurwa zvakananga kune electrophoresis;kushanda kuri nyore uye nyore.
Kit application
PCR kukwidziridzwa kwezvimedu zveDNA, kunyora DNA, DNA sequencing,PCR pamwe nemuswe
Storage uye Sherufu upenyu
Inogona kuchengetwa kwenguva yakareba pa -20 ° C;kuti ishandiswe kakawanda, inogona kuchengetwa pa4 ° C kwenguva pfupi. Tichifunga nezve motto iyi, tave imwe yevagadziri vehunyanzvi hwekugadzira tekinoroji, vanodhura, uye vanokwikwidza mutengo weMutengo Wakanakisisa weTaq DNA Polymerase, Kuti tiwane zvakanakira kudzokororwa, kambani yedu iri kuwedzera nzira dzedu dzekudyidzana kwepasirese maererano nemhando yepamusoro yevatengi, nekukasira, kuburitsa kwepamusoro-soro kwevatengi.
Best Price forChina 2X Taq Mstermix uye Mmlv Taq, Kambani yedu yakagara ichisimbirira pamusimboti webhizinesi we "Hunhu, Kutendeseka, uye Mutengi Kutanga" iyo yatakawana nayo kuvimba nevatengi kubva kumba nekune dzimwe nyika.Kana iwe uchifarira zvekutengesa kwedu, haufanirwe kuzeza kutibata kuti uwane rumwe ruzivo.
