Isu tinoomerera mukati meiyo dzidziso yekuvandudzwa kwe 'Yakakwira mhando, Kuita, Kuvimbika uye Kudzika-pasi-pasi-kushanda maitiro' kuti tikupe iwe akasarudzika mupi wekugadzirisa we2019 Yezvino Dhizaini China Vtm Kits ine 10ml Tube 3ml Phenolic Red Vtm Kits, Isu tinotambira nemoyo wese shamwari kubva pasirese pasirese kuti tibatane nesu kwenguva refu.
Isu tinoomerera mukati meiyo dzidziso yekusimudzira ye 'Yakakwira mhando, Kuita, Kuvimbika uye Kudzika-kune-pasi kushanda nzira' kukupa iwe akasarudzika mupi wekugadzirisaChina Viral Transport Medium, Vrius Transport Media, Basa redu nderekuunza zvinogara zvichikosha kukosha kune vatengi vedu uye vatengi vavo.Kuzvipira uku kunopinda mune zvese zvatinoita, zvichititungamira kuti tirambe tichigadzira nekuvandudza zvigadziriso zvedu uye maitiro ekuzadzisa zvaunoda.

Zvinotsanangurwa

50 Preps

Buffer FL1: Inopa nharaunda ye enzymatic hydrolysis yematishu.

Foregene Protease Plus: Enzymatic digest tissue samples munzvimbo yeBuffer FL1.

Buffer FL2: Ita kuti Foregene Protease Plus uye ipe DNA yekurodha nharaunda.

Buffer PW: Bvisa tsvina yakaita seprotein uye RNA muDNA.

Buffer WB: Bvisa maion emunyu asara muDNA.

Buffer EB: Elute iyo DNA pane yekuchenesa column membrane.

DNA-chete Column: Kunyanya adsorb iyo genomic DNA mune lysate.

Kit zvikamu

Zvimiro&zvakanakira

-Kwete kusvibiswa kweRNase: Iyo DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva kune genomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.

-Kukurumidza kukurumidza: Foregene Protease Plus ine basa rakakwirira kudarika maprotease akafanana, uye inogaya sampuli dzenyama nokukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati meawa 2-5.

-Kusununguka: Iyo centrifugation inoitwa pane tembiricha yekamuri, uye hapana chikonzero che4 ° C yakaderera-kupisa centrifugation kana ethanol kunaya kweDNA.

-Kuchengeteka: hapana organic reagent kutorwa kunodiwa.

-Hunhu hwepamusoro: Iyo yakabviswa genomic DNA zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, iyo inogona kusangana nezvinodiwa zvekuedza kwakasiyana.

Kit application

Inokodzera kudhirowa uye kucheneswa kwegenomic DNA kubva kune formaldehyde-yakagadziriswa samples uye parafini-yakamisikidzwa tishu samples.

Workflow

Storage uye Sherufu upenyu

-Iyi kititi inogona kuchengetwa kwemwedzi gumi nemaviri pasi pemamiriro akaoma pakushambidzika kwekamuri (15-25 ° C);kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa pa2-8°C.

Ongorora: Kana yakachengetwa pane tembiricha yakaderera, mhinduro yacho inonangana nekunaya.Usati washandisa, iva nechokwadi chekuisa mhinduro mukiti pane tembiricha yekamuri kwenguva yakati.Kana zvichidikanwa, preheat mubhati yemvura ye 37 ° C kwemaminitsi gumi kuti iparadze mvura, uye isanganise isati yashandiswa.

-Forgene Protease Plus mhinduro ine yakasarudzika formula inoshanda kana yakachengetwa mukamuri tembiricha kwenguva yakareba (3 mwedzi);inonyanya kushanda uye yakagadzikana kana yakachengetwa pa 4 ° C, saka inokurudzirwa kuichengeta pa 4 ° C, yeuka kuti usaichengeta pa -20 ° C. Isu tinoomerera mukati medzidziso yekuvandudzwa kwe 'High high quality, Performance, Sincerity and Down-to-earth working approach' kuti ikupe iwe mupi wepamusoro wekugadzirisa 2019 Latest Design China Vts 30mtt Kirts Kirts Kirts. gamuchirai zvakanaka shamwari dzinobva pasi rese kuti dzishande nesu pahwaro hwenguva refu yekubatsirana.
2019 Yazvino DhizainiChina Viral Transport Medium, Vrius Transport Media, Basa redu nderekuunza zvinogara zvichikosha kukosha kune vatengi vedu uye vatengi vavo.Kuzvipira uku kunopinda mune zvese zvatinoita, zvichititungamira kuti tirambe tichigadzira nekuvandudza zvigadziriso zvedu uye maitiro ekuzadzisa zvaunoda.