Direct PCR
Chii chinonzi Direct PCR
Direct PCR ndiyo nzira yeDNA amplification yakananga kubvaropa, mhuka mhuka,muswe wegonzo,sero,nzeve yemakonzo,mbizi hove, mazai ehove,dyara mashizha,mbeukana imwe yekutanga biological tissue sample pasina kuita DNA yekuzviparadzanisa nevamwe uye matanho ekuchenesa.Yakananga PCR tekinoroji inogona kuderedza zvakanyanya nguva yekuyedza, uye mutengo mu genotyping uye yakakwirira-huwandu mapurojekiti.Inopawo sarudzo iri nani kana watarisana nematambudziko ekusimudzira huwandu diki hwesample uko nhanho yekuchenesa inogona kutungamira mukurasikirwa kwemuenzaniso.
FOREGENE's PCR Mix ine simba rekuramba kushushikana, nekuda kwe FOREGENE simba rine patent yeTaq enzyme( mvumo yepamutemo: ZL20161034512.0 (China), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Japan)Yayo Taq enzyme inoshandisa DNA recombination tekinoroji kubatanidza iyo DNA inosunga chimiro dunhu rearchaeal nucleic acid inosunga mapuroteni neinodzivirira kupisa Taq DNA polymerase kuti ivake template DNA nekuwedzera hukama.Iyo yakavandudzwa inopisa-kutanga Taq inochengetedza mabasa akasiyana-siyana epakutanga DNA polymerase.Iyi Taq enzyme inogona kusunga ku template DNA uye kutanga DNA synthesis mu low concentration templates uye zvakaoma nharaunda.
For muenzaniso,itzviri nyore sekuwedzera yakocell,chirimwa (shizha diki, mudzi kana mhodzi) kana mhuka yemuenzaniso kunebuffer, uye nekuwedzera iyo yakasarudzikamaster mix withzvakanangamaprimers muPCR machubhu uye achimhanyisa kuburikidza neanopisa cycler.Kushanda kweKit kwakanakira chiyero chikuru chekuongorora uko kuchengetedza nguva kwakakosha zvakanyanya.
Iyo PCR reaction system ine UNG enzyme uye dUTP inosarudzwa zvinoenderana nezvinodiwa zvekuedza, izvo zvinogona kunyatso kudzivirira kusvibiswa kunokonzerwa nePCR amplification zvigadzirwa.
Zvakanakira zveDPCR chaiyo
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| Direct PCR | Nzira yechinyakare (RNA kucheneswa+ Coventional PCR) |
| Kushandiswa kwezvinhu | Kudyiwa kwetishu | Zvishoma | Zvakawanda |
| Kugadzirira template | Rudzi rwezvinhu | Hapana kugaya kunodiwa | Kukuya samples |
| Operation | Imwe-chubhu mhando, 10min | kushanda kwakaoma, 1h | |
| Flux | 1-96 | 1-24 | |
| PCR maitiro | reaction system | Yakagadzirirwa 2 × PCR musanganiswa | Dntp, MgCl2,10 × PCR Buffer, Taq enzyme, |
Application
01 Kuzivikanwa kwepathogenic microorganisms
02 Genetically yakagadziridzwa chitupa, genotyping
03 Multiplex PCR / SNP kuongororwa / PCR-RFLP
04Sequencing/cloning
Product Series--Ropa Direct PCR Series
| Series | Product Name | Zvinotsanangurwa | Catalog Number | Storage Conditions |
| Ropa Rakananga PCR Series | 200 × 20μl rxns | TP-04111 | -20 ℃ | |
| 2000 × 20μl rxns | TP-04113 | |||
| 200 × 20μl rxns | TP-04121 | -20 ℃ | ||
| 2000 × 20μl rxns | TP-04123 | |||
| 200 × 20μl rxns | TP-04211 | -20 ℃ | ||
| 2000 × 20μl rxns | TP-04213 | |||
| 200 × 20μl rxns | TP-04221 | -20 ℃ | ||
| 2000 × 20μl rxns | TP-04223 |
