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Mana Main Solutions YePCR Chigadzirwa Kusvibisa Kudzora
1: Tsiva zvinhu zvekuyedza munguva Seta (NTC) kutonga kwakashata uye dzokorora kakawanda.Kana zvangoonekwa kuti mune PCR chigadzirwa kusvibiswa murabhoritari, tsiva zvese zvekuyedza nenguva.Zvakadai se: dilute zvakare uye gadzirira maprimers, re-sterilize iyo pipette tip, E ...Verenga zvimwe -
Maviri maviri-anoshanda RT-PCR ma enzymes
Traditional reverse transcriptases haigoni kushivirira tembiricha yepamusoro (tembiricha yakakwana yeMMLV chiitiko i37-50°C, uye AMV i42-60°C).Iyo yakanyanya kuomesesa hutachiona RNA haigone kudzoreredza zvinobudirira kunyorwa mu cDNA pakadzika tembiricha, zvichikonzera kugona kwekuona Kuderedza.Tra...Verenga zvimwe -
Nucleic acid isothermal amplification tekinoroji
PCR ndiyo inonyanya kushandiswa nucleic acid amplification tekinoroji uye inoshandiswa zvakanyanya nekuda kwekunzwa kwayo uye kujeka.Nekudaro, PCR inoda kudzokorora kupisa denaturation uye haigone kubvisa zvipingamupinyi zvekuvimba nezviridzwa nemidziyo, iyo inomisa kushanda kwayo mukiriniki ...Verenga zvimwe -
Chii chinonzi mRNA vaccine?
Chii chinonzi mRNA vaccine Iyo mRNA yekudzivirira inoendesa RNA kumaseru emuviri kuratidza uye kugadzira maprotein antigens mushure mekugadziriswa kwakakodzera mu vitro, nekudaro zvichitungamira muviri kuburitsa mhinduro yekudzivirira kurwisa antigen, nekudaro kuwedzera bod...Verenga zvimwe -
Nhoroondo yePCR Technology
PCR (polymerase chain reaction) ndeimwe ye-in-vitro DNA amplification technologies, ine nhoroondo yemakore anopfuura makumi matatu.PCR tekinoroji yakatangwa naKary Mullis wekuCetus, USA muna 1983. Mullis akanyorera PCR patent muna 1985 uye akaburitsa bepa rekutanga redzidzo rePCR paSainzi zvakafanana...Verenga zvimwe -
Kuonekwa kutsva kwekoronavirus, kubva kunucleic acid, antibody kuenda kuantigen, maitiro ekusarudza kurongeka kwedenda?
Denda rachinja nyika.Munyika yose, hurumende dzenyika dzose dziri kutarisana nematambudziko makuru mukudzivirira nekudzora denda.Munguva yeCOVID-19 denda, China iri mumatanho mana ekudzivirira uye mhinduro sisitimu (kudzivirira, kuona, kutonga uye Kiyi yekubvisa ...Verenga zvimwe -
Nzira ina dzekudzivirira PCR zvigadzirwa kusvibiswa
Kuvaka SOP Systerm Gadzira PCR kuyedza SOP kumisa maitiro evashandi vekuyedza.Vanoedza vanonyatsotevedzera maitiro ekushanda, uye kuderedza PCR kusvibiswa kunogona kukonzerwa nevanhu kana kudzivirira kuitika kwekusvibiswa mukushanda.Mukuwedzera...Verenga zvimwe -
Nzira yekuvandudza sei kushanda kweLncRNA reverse transcription?
Lead Yakareba isiri-coding RNA, lncRNA iRNA isiri-coding ine hurefu hunodarika mazana maviri nucleotides, kazhinji pakati pe200-100000 nt.lncRNA inogadzirisa gene expression pa epigenetic, transcription uye post-transcriptional mazinga, uye inotora chikamu mu X chromosome kunyarara, genome imprinting uye chroma...Verenga zvimwe -
Inogona kupfuudzwa kubva kumunhu kuenda kumunhu mumasekonzi gumi nemana.Chii chakaitika kune "kunyanya kutapurirana" Delta dhiri muZhong Nanshan Pass?
Yakanyorwa sosi: WuXi AppTec timu mupepeti MuGuangzhou, China, mapurisa ane basa rekubatsira kuferefeta kwedenda akaburitsa vhidhiyo yekutarisa: Muresitorendi imwe chete, vaviri vakapinda muimba yekugezera mumwe mushure memumwe pasina kana kubata.Masekonzi gumi nemana chete ekubatana...Verenga zvimwe -
SNP molecular labeling uye kuonekwa
Mushure mekunge nyanzvi yekuAmerica Eric S. Lander yataura zviri pamutemo imwe nucleotide polymorphism (SNP) seyechitatu-chizvarwa molecular marker muna 1996, SNP yakashandiswa zvakanyanya mukuongorora kwehupfumi hwemagariro ehutano, biological genetic linkage mepu, uye vanhu pathogenic gene kuongorora., D...Verenga zvimwe -
Popular Science |Maitiro ekuona iyo coronavirus nyowani neqPCR
COVID-19 chirwere chinotapuriranwa chinokonzerwa neSevere Acute Respiratory Syndrome Coronavirus Type 2. Kana munhu aine utachiona, zviratidzo zvinonyanyozivikanwa zvinosanganisira fivha, kukosora, uye kutadza kufema zvakanaka.Iwo masampula anoshandiswa pakuyedza anogona kuunganidzwa ne nasopharyngeal swabs kana oropharyngeal swabs.Chii ini...Verenga zvimwe -
Uchiri kushandisa tekinoroji yakagadzirwa makore makumi matatu apfuura kubvisa RNA?
Uchiri kushandisa Trizol kubvisa RNA?Gosh, usandirega ndichinetseka nezvehutano hwako!Trizol yave kuda kuiswa marginalized,usandiudze kuti uchiri murima here?Chinhu chikuru cheTrizol reagent ndeye phenol, uye organic reagents senge chloroform inowedzerwawo muexperimen...Verenga zvimwe
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