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Ndeupi musiyano uripo pakati pePCR, RT-PCR, qPCR, uye RT-qPCR
PCR inzira inoshandiswa kukudza DNA kubva padiki diki reDNA template.RT-PCR inoshandisa reverse transcription kugadzira template yeDNA kubva kuRNA sosi inogona kukwidziridzwa.PCR uye RT-PCR ndiwo maitiro ekupedzisira, nepo qPCR neRT-qPCR vachishandisa kinetics yechiyero chechigadzirwa synt...Verenga zvimwe -
Iko kumberi kweKuwanikwa Kwezvinodhaka: Kutarisa RNA neMadiki Molecules
Pfizer's mRNA yekudzivirira yeCOVID yakadzora chishuwo chekushandisa ribonucleic acid (RNA) sechinangwa chekurapa.Nekudaro, kunanga RNA nemamorekuru madiki kunonetsa zvakanyanya.RNA inongova nematombo mana ekuvaka: adenine (A), cytosine (C), guanine (G), uye uracil (U) inotsiva thymin ...Verenga zvimwe -
Chii chinonzi nucleic acid zvinodhaka?
“Nucleic acid drugs” inoshandisa “nucleic acid,” iyo inoreva zvinhu zvakadai seDNA neRNA zvinodzora ruzivo rwemajini, semishonga.Izvi zvinobvumidza kunangana kwemamorekuru akadai semRNA uye miRNA asingakwanise kunangana neakadzika mamorekuru uremu zvinodhaka uye antibody mishonga, uye kune g...Verenga zvimwe -
Chimwe chiitiko nezve siRNA muRT-PCR
1. Ruzivo rwekutanga (kana iwe uchida kuona chikamu chekuyedza, ndapota tumira zvakananga kune chikamu chechipiri) Sekubuda kwekuita kweyakajairwa PCR, Nguva chaiyo PCR inonyanya kutarisa shanduko yehuwandu hwechigadzirwa chekusimudzira mune yega yega kutenderera kwePCR amplification. reaction in real time thr...Verenga zvimwe -
RT-PCR yekuedza maitiro system optimization nzira yakadzama pfupiso
一、 Wedzera kunzwisiswa kweiyo reaction system: 1. Isolate yemhando yepamusoro RNA: Yakabudirira cDNA synthesis inobva kumhando yepamusoro RNA.Yepamusoro-soro RNA inofanira kunge izere-kureba uye isina reverse transcriptase inhibitors seEDTA kana SDS.Hunhu hweRNA hunosarudza huwandu hwe ...Verenga zvimwe -
Zadzisa PCR primer dhizaini uye PCR ruzivo
Primer design nheyo (99% matambudziko anogona kugadziriswa) 1. Primer urefu: Bhuku rinoda 15-30bp, kazhinji inenge 20bp.Mamiriro chaiwo ari nani kuve 18-24bp kuve nechokwadi cheiyo chaiyo, asi iyo yakareba zviri nani, yakarebesa primer zvakare inoderedza iyo chaiyo, uye kuderedza goho.2. Prim...Verenga zvimwe -
Chengetedzo yeqRT-PCR inounganidzwa naChiremba mukuru zvinorwadza
Wese munhu ari kutaura nezve musimboti wekuyedza qRT-PCR, dhizaini yekutanga, dudziro yemhedzisiro, nezvimwe, asi ndinofunga ndinofanira kugovana newe kuyedza kushanda kweqRT-PCR.Idiki, asi ndeye maresults.Tisati taita qRT-PCR, tinoda kuve nekunzwisisa kwakajeka kwedu ...Verenga zvimwe -
Chengetedzo yeRT-qPCR
RT-qPCR kuyedza inosanganisira RNA kudhirowa uye kuongororwa kwemhando, reverse transcription uye qPCR matanho matatu, nhanho imwe neimwe ine zvakawanda zvekuchengetedza, isu tichazivisa zvakadzama pazasi.Ⅰ.RNA yemhando yekuongorora MuRT-qPCR kuyedza, mushure mekupedzwa kweRNA kudhirowa, ...Verenga zvimwe -
Sumo yakakwana kune chaiyo nguva PCR
1. Kunzwisisa kwekutanga Panguva ino, tinofanira kunzwisisa mamwe mazano uye mazwi, kuitira kuti tisaita zvikanganiso pamberi pevakuru vedu, zvakadai se: Q: Ndeupi musiyano pakati peRT-PCR, qPCR, Real-time PCR, uye chaiyo-nguva RT-PCR?Mhinduro: RT-PCR ndeye reverse transcription PCR (reverse t...Verenga zvimwe -
PCR, akawanda PCR, In situ PCR, Reverse PCR, RT-PCR, qPCR (1) - PCR
PCR, akawanda PCR, In situ PCR, Reverse PCR, RT-PCR, qPCR (1)– PCR Tichagadzirisa pfungwa, nhanho uye ruzivo rweakasiyana PCR Ⅰ.PCR Polymerase Chain Reaction, inodaidzwa kunzi PCR, tekinoroji yebhayoloji inoshandiswa kukudza zvimedu zveDNA.Inogona kutorwa se ...Verenga zvimwe -
qRT-PCR yekuedza musimboti
RT-qPCR inogadzirwa kubva kune yakajairwa PCR tekinoroji.Iyo inowedzera fluorescent makemikari (fluorescent dhayi kana fluorescent probes) kune yechinyakare PCR reaction system, uye inoona iyo PCR annealing uye yekuwedzera maitiro munguva chaiyo zvinoenderana neavo akasiyana mwenje maitirwo.Chiratidzo cheFluorescent ...Verenga zvimwe -
RT-PCR yekuedza maitiro system optimization nzira yakadzama pfupiso
Ⅰ.Wedzera kunzwisiswa kwemaitiro ekugadzirisa: 1. Kuparadzanisa yakakwirira-yepamusoro RNA: Kubudirira cDNA synthesis inobva kune-high-quality RNA.Yepamusoro-mhando RNA inofanirwa kuve nechokwadi chenguva yakareba uye haina inhibitors isina maenzayimu ekurekodha, akadai seEDTA kana SDS.The Quali...Verenga zvimwe
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