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Kuvaka SOP System

Gadzira PCR yekuyedza SOP kumisa maitiro evashandi vekuyedza.

Nzira ina dzekudzivirira PCR zvigadzirwa kusvibiswa1

Vanoedza vanonyatsotevedzera maitiro ekushanda, uye kuderedza PCR kusvibiswa kunogona kukonzerwa nevanhu kana kudzivirira kuitika kwekusvibiswa mukushanda.Pamusoro pezvo, muyedzeri anofanirwa kuve neruzivo rwehunyanzvi uye hunyanzvi hunoenderana, kusanganisira hunyanzvi hwekushandisa zvine chekuita nemidziyo, kujekesa maitirwo ese ebasa, kugona nzira dzekurapa dzekusvibisa uye nzira dzekudzora mhando yerabhoritari, uye kukwanisa kududzira nemazvo mhinduro dzebvunzo.

Kuvaka Standard PCR Laboratory

Nzira ina dzekudzivirira PCR zvigadzirwa kusvibiswa2

Iyo PCR rabhoritari yakakamurwa kuita nzvimbo ina mumusimboti, dzinoti reagent nzvimbo yekugadzirira, sampuro yekugadzirisa nzvimbo, nzvimbo yekusimudzira, uye amplification chigadzirwa chekuongorora nzvimbo.Nzvimbo mbiri dzekutanga inzvimbo dzekutanga-amplification, uye nzvimbo mbiri dzekupedzisira inzvimbo dzemashure-amplification.Iyo pre-amplification zone uye post-amplification zone inofanira kunyatsopatsanurwa.Zvishandiso zvekuyedza, reagents, bepa rekurekodha, zvinyoreso, zvinhu zvekuchenesa, nezvimwewo zvinogona kungoyerera kubva kunzvimbo ye-pre-amplification kuenda kunzvimbo ye-post-amplification, kureva, kubva kunzvimbo yekugadzirira reagent, nzvimbo yekugadzira sampuli, nzvimbo yekusimudzira, uye amplification chigadzirwa chekuongorora nzvimbo, uye haifanire kuyerera ichidzokera kumashure.Kuyerera kwemhepo murabhoritari kunofanirwawo kuyerera kubva kunzvimbo ye-pre-amplification kuenda kunzvimbo ye-post-amplification, kwete kune reverse direction.

Deredza matanho ekuedza

Kana marabhoritari ichingoona PCR uye kuzivikanwa, zvinokurudzirwa kushandisa fluorescent quantitative PCR pachinzvimbo chePCR yakajairika.

Nzira ina dzekudzivirira PCR zvigadzirwa kusvibiswa3

Fluorescence quantitative PCR yekuona mhedzisiro inogona kuunganidzwa uye kuongororwa nemasaini efluorescent, saka hapana chikonzero chekuvhura chivharo che electrophoresis mushure mekuita, iyo inodzivirira kusvibiswa kwePCR zvigadzirwa zvinokonzerwa nekudonha kwemaitiro ekugadzira kugadzira aerosols.Kana iwe ukawedzera huwandu hwemavharo ekuvhura panguva yekurodha nhanho yegel electrophoresis, kusvibiswa kweaerosol kunogona kuitika.Inokurudzirwa kusimudzira kushandiswa kwehuwandu hwePCR uye zvishoma nezvishoma kutsiva qualitative PCR.

Adopt UNG anti-kusvibisa system

Iyo UNG anti-PCR chigadzirwa kusvibiswa system inoshandiswa kune PCR maitiro.

Iyo sisitimu inoshandisa dUTP pachinzvimbo che dTTP.Mushure mekuita kwePCR, zvese zvigadzirwa zvePCR (DNA zvidimbu) zvinosanganiswa nedUTP;muchikamu chinotevera chePCR reaction, iyo UNG enzyme yakawedzerwa kune system inoiswa pa 37 ° C kwemaminetsi mashanu pamberi pePCR, inogona kuve yakananga Degrade zvese zvidimbu zveDNA zvine dUTP, uye wozoita PCR reaction.Izvi zvinogona kubvisa zvachose kusvibiswa kweaerosol kunokonzerwa nePCR zvigadzirwa.Mhedzisiro inoratidzwa mumufananidzo uri pasi apa:

Nzira ina dzekudzivirira PCR zvigadzirwa kusvibiswa4Ongorora: Kune yakananga PCR yakatevedzana, unogona kusarudza akatevedzana zvigadzirwa zveanopesana nePCR chigadzirwa kusvibisa system yeFJ Biotech.

Suggest

Kune marabhoritari anoita hukuru-hukuru genotyping bvunzo, zvinokurudzirwa zvakanyanya kushandisa iyo UNG anti-PCR chigadzirwa chetsvina system yekuyedza reagents mukuwedzera pakuvakwa kwemarabhoritari ane musoro.

Chiyeuchidzo: Kushandiswa kwegadziriro iyi hakugone kubvisa kusvibiswa kwechigadzirwa chePCR kwakatokonzerwa.Naizvozvo, iyo UNG system inofanirwa kushandiswa pakutanga kwebvunzo yakakodzera, uye iyo UNG system inofanirwa kugara ichishandiswa kukwidziridza PCR, kudzivirira kusvibiswa kwePCR zvigadzirwa.False positive.

Zvinokurudzirwa kushandisa Direct PCR-UNG sisitimu yeForge Biotech paunenge uchiita bvunzo dzakakura, senge: Plant Leaf Direct PCR Kit-UNG;

Dyara Mbeu Yakananga PCR Kit-UNG;

Animal Tissue Direct PCR Kit-UNG;

Mouse Muswe Wakananga PCR Kit-UNG;

Zebra Hove Yakananga PCR Kit-UNG.

Iyi nhevedzano yemakiti kubva kuForegene haingogone chete kuita PCR yekuona nekukurumidza uye pamwero mukuru, asi zvakare zvinobudirira kudzivirira nekudzora PCR chigadzirwa kusvibiswa.


Nguva yekutumira: Jul-30-2021