Zvakawanda sei iwe unoziva

Nezve Nucleic acid kubviswa

Kutanga uye kupera kwekunatswa nucleic acid

Zvose zvakaoma pakutanga, kucheneswa nucleic acid ndiyo yakanyanya

Kutanga kwekuedza kwema molecular, kune zviedzo zvinotevera

Kubudirira kana kukundikana kune zvakakosha.

Trace/ultra-trace UV spectrophotometer inofarirwa nevazhinji vaongorori vesainzi nekuti inokwanisa kuona nucleic acid yakasungwa uye mapuroteni uye masara emunyu ion zviri nyore, nekukurumidza uye zvine hupfumi.

Sezvatinoziva tese, A260 zvinoreva nucleic acid absorbance OD value, A280 inoreva protein concentration absorbance OD value, A230 inoreva salt ion concentration absorbance OD value, saka A260 inogona kushandura nucleic acid concentration, A260/280 inoreva maprotein residue, A260/230 inoreva salt ion residue, asi iyi ingori nhamba yezviyero zvawanikwa, asi iyi ingori chiyero chetsvakiridzo.zvakajairika” kutenda kuti inogona kutsanangura kuchena kweDNA neRNAkumwero wakati.Haisi iyo chete chiyero chekuzivikanwa kweiyo nucleic acid goho uye kuchena, inongoshandiswa sereferensi, uye haisi "yegoridhe chiyero".

Iyo Thermo Fisher ND-2000 bhuku rinosimbisa kuvimbika kwemhedzisiro yebvunzo

Chikonzero ndechekuti mibairo yakawanikwa nekushandisa micro/ultramicro UV spectrophotometer inongoratidza goho uye kuchena kwenucleic acid kubva padivi, uye kune zvakawanda zvinofurira zvinhu (optical path, sample volume, sample concentration, pH value, mamwe maion anokanganisa kuyerwa kwekuyeresa, etc.) , iyo yakayerwa kukosha kweOD haina chokwadi.Panguva imwecheteyo, nekuda kwekushaikwa kweiyo chaiyo yeabsorption value determination material, imwe-stranded DNA, kaviri-stranded DNA, RNA, dNTPs, uye mamwe mapuroteni ose ane absorption peaks pa 260nm wavelength, uye kusungirirwa kunotarwa neA260 chete haisi iyo chaiyo DNA kana RNA.Kuisa pfungwa, uye kutendeseka kwayo uye kuderedzwa hakugoni kutongwa.

Saka maitiro ekutonga kunaka kweyedu yakacheneswa nucleic acid?Pamusoro pekushandisa micro/ultramicro UV spectrophotometer yekuona, nzira dzakadai seagarose gel electrophoresis dzinodiwawo kuratidza nemeso kuchena uye kuvimbika kweiyo nucleic acid, uye kuratidza kusungirirwa kune imwe nhanho.Neiyi nzira, iyo nucleic acid goho uye kuchena kunowanikwa kubva pakuona mhedzisiro yenzira dzakasiyana-siyana zvinovimbika.

Yekuedza chati yekuenzanisa

Genomic DNA yeH1299 masero akatorwa achishandisa kambani A's DNA Extraction kits, uye kusvibiswa kwakakosha kwekusachena kwakaonekwa, zvichikonzera kukwirira kweOD.

(OD kuyerwa kukosha uye inoenderana electropherogram)

Evaluation index

Pane here "yegoridhe chiyero" cheiyo nucleic acid goho uye yemhando yekuyedza?

Sekuziva kwedu, hapana nzira iri nyore, inokurumidza uye yakachipa.Ingave spectrophotometer, gel electrophoresis, kana mass spectrometry, zvose zvinoratidza kusungirirwa uye kuchena kwe nucleic acid mumhinduro kubva kune zvakasiyana-siyana, uye dzinoda kutongwa nekutaura kune mumwe nemumwe.

Yakanakisa evaluation index inogara iripoiyo yekutevera yekuedzwa application.Izvo hazvikanganisi kushanda kwe reverse transcription uye PCR amplification.Kuwana yakavimbika amplification zvigadzirwa uye Ct kukosha, uye kuwana kutevedzana kwakazara nekutevedzana kwakabudirira nucleic acid kudhirowa.

Kupfupisa, maonero ereshiyo-chete dzidziso anofanira kupikiswa nemaonero e "kushandisa zvakanaka kuzasi kwechiyedzo mhedzisiro seyakanaka yekubvisa paramita"!

Yakakurudzirwa Foregene DNA RNA yekuwedzera kits kuti uwane yakakwira DNA/RNA kuchena:

https://www.foreivd.com/reagent/dna-isolation-series/

https://www.foreivd.com/reagent/rna-isolation-series/