Kupisa-kutanga Taq enzyme inoshandiswa zvakanyanya.Kuenzaniswa neyakajairwa DNA polymerase, inopisa-kutanga Taq enzyme inogona kunyatso dzivirira imwe isiri-chaiyo amplification uye kuumbwa kweprimer dimers, uye inogona kunyatso kuvandudza mwero wekubudirira kwechinangwa chekusimudzira gene.Kunyanya mumunda wekuongororwa kwemajini, kupisa-kutanga Taq enzyme yakaonekwa seyakajairwa chiyero muindasitiri, uye yakajairwa DNA polymerase haifanirwe kushandiswa.Sezvinoonekwa kubva pamusoro, inopisa-kutanga Taq enzymes inoshandiswa zvakanyanya.Parizvino, kune akawanda mabhureki ekupisa-kutanga Taq enzymes pamusika wepamba, asi hapana akawanda anopisa-anotanga Taq enzymes ane emhando yepamusoro.Takatarisana nezvakawanda zvinopisa-kutanga Taq enzyme zvigadzirwa, isu tinofanira kusarudza sei?

1. Sarudza inopisa-kutanga Taq enzyme ine yakakwirira amplification kunyatsoshanda

PCR amplification kunyatsoita zvine hukama nekuita kweTaq enzyme.Mushure meiyo yakanaka Taq enzyme reaction system yakagadziridzwa, iyo amplification inobudirira iri pamusoro pe95%, uye iyo yekuwedzera huwandu hweyekutanga template huwandu hwakafara.Kukwidziridzwa kunogutsa kunogona kuwanikwa kana chinangwa chejini chemukati chadzikira, uye hazvisi nyore kuve nechepfu kana huwandu hwetemplate hwakwira, uye nguva yekuwedzera yekuwedzera yakareba.Kune iyo Taq enzyme ine kusaita zvakanaka, kunyangwe iyo maitiro ekuita yakagadziridzwa kakawanda, iyo amplification ichiri isingasviki 90%, iyo "S" chimiro cheamplification curve haisi pachena, mutsetse idiki, uye curve yakati sandara.Kana huwandu hwetemplate hwakaderera, haugone kukwidziridzwa, uye kana huwandu hwetemplate hwakwira, iyo amplification maitiro haina kunaka.Naizvozvo, kusarudzwa kweDNA polymerases ine yakakwirira amplification kushanda kwakakosha mukubudirira kwePCR neqPCR.

2. Sarudza kupisa-kutanga Taq enzyme ine simba rakasimba re enzyme

Iyo enzymatic simba reTaq enzyme ine hukama nekuita kweamplification.Kazhinji, kusimba kwesimba reenzymatic reiyo inopisa-kutanga Taq enzyme, iyo yakareba iyo exponential yekukura nguva yePCR kukwidziridzwa, iyo yakajairika 'S-yakaita' curve, iyo yakakwirira kukosha kwechiratidzo chefluorescence, uye iyo yakanyanya kukodzera kuongororwa kwePCR yakawanda.Brand DNA polymerases ine simba risina simba enzymatic inogona kutsigira 2-plex maitiro.Paunenge uchiita 3-plex reactions, iyo amplification curve yakadzikira, iyo fluorescence chiratidzo kukosha yakaderera, uye hapana yakajairika amplification curve, saka mhedzisiro yakaoma kutonga.

3. Sarudza kupisa-kutanga Taq enzyme nekunzwa kwakanyanya

Kazhinji kutaura, DNA polymerase ine yakakwira amplification kunyatsoita uye yakanyanya senitivity, asi kune zvakare kusawirirana.Kana iyo yakanangwa gene kuwanda kweiyo sampuli kuti ikwidziridzwe yakaderera, zvinokurudzirwa kuyedza iyo amplification senitivity yeTaq enzyme.Iyo inonyanya kuzivikanwa nzira ndeyekuita gumi-kupeta kana 5-kupeta gradient dilution yeinonangwa gene plasmid fragment, kuita PCR kucherechedzwa pazasi dilution, uye sarudza inopisa-kutanga Taq enzyme ine yakakwirira yekuona senitivity.

Zvinogona kuonekwa kubva pamusoro apa kuti vatsvakurudzi vanofanira kusarudza maererano nezvavanoda kuedza uye mamiriro emari.Zvakanakisa kuita gradient dilution amplification kuyedza kuona iyo amplification kunyatsoita uye kunzwa kweiyo inopisa-yekutanga Taq enzyme.

Muenzaniso weForegene's Taq DNA Polymerase:

Foreasy HS Taq DNA Polymerase

Tsanangudzo

Foreasy HS Taq DNA Polymerase iDNA polymerase inoratidzwa muEscherichia coli engineering mabhakitiriya nejene recombination tekinoroji.Iyo enzyme inosanganiswa neyakasarudzika reaction buffffer, iyo inoita kuti chigadzirwa chirambe chiripo uye chienderane, uye chinogona kushandisa zvakananga sampu lysate (Forgene Lysis system) se template yekuona maitiro.

Application

Qualitative PCR uye kuwanda kwePCR kuona kweakacheneswa matemplate uye asina kucheneswa matemplate.

Quality Control

1.No exogenous nuclease chiitiko chaonekwa

2.PCR nzira yekuona yakasara genomic DNA pasina muenzi

3.Inokwanisa kukwirisa zvine mutsindo majini e-single-copy muGenome yemunhu

4.Kuchengeta pakamuri tembiricha kwevhiki,noobvious chiitiko chinoshanduka

Ruzivo rwechigadzirwa: https://www.foreivd.com/foreasy-hs-taq-dna-polymerase-product/