Gadzira PCR yekuyedza SOP kumisa maitiro evashandi vekuyedza.
Muongorori anonyatso kutevedzera maitiro ekushanda, uye anoderedza kusvibiswa kwePCR kunogona kukonzerwa nezvinhu zvevanhu kana kudzivirira kuitika kwekusvibiswa.Pamusoro pezvo, muyedzeri anofanirwa kuve neruzivo rwehunyanzvi uye hunyanzvi hunoenderana, kusanganisira hunyanzvi hwekushandisa zvine chekuita nemidziyo, kujekesa maitirwo ese ebasa, kugona nzira dzekurapa dzekusvibisa uye nzira dzekudzora mhando yerabhoritari, uye kukwanisa kududzira nemazvo mhinduro dzebvunzo.
Gadzira yakajairika PCR marabhoritari.
PCR rabhoritari yakakamurwa kuita nzvimbo ina mumusimboti, dzinoti reagent nzvimbo yekugadzirira, sampuli yekugadzirisa nzvimbo, nzvimbo yekusimudzira, uye amplification chigadzirwa chekuongorora nzvimbo.Nzvimbo mbiri dzekutanga inzvimbo dzekutanga-amplification, uye nzvimbo mbiri dzekupedzisira inzvimbo dzemashure-amplification.Iyo pre-amplification zone uye post-amplification zone inofanira kunyatsopatsanurwa.Zvishandiso zvekuyedza, mareagents, bepa rekurekodha, zvinyoreso, zvinhu zvekuchenesa, nezvimwewo, zvinongoyerera kubva kunzvimbo ye-pre-amplification kuenda kune post-amplification nzvimbo, kureva, kubva kune reagent nzvimbo yekugadzirira → sampuli yekugadzirisa nzvimbo → amplification nzvimbo → amplification yekuongorora chigadzirwa nzvimbo, uye haifanirwe kuyerera ichidzokera kumashure.Kuyerera kwemhepo murabhoritari kunofanirwawo kuyerera kubva kunzvimbo ye-pre-amplification kuenda kune post-amplification, uye kwete kuyerera kumashure.Iyo yakakodzera PCR laboratori dhizaini inoratidzwa pazasi:
Mufananidzo A: Yakanaka PCR labhoritari setup modhi ine kumanikidza kwakashata mukamuri yebuffer
Mufananidzo B: Yakanaka PCR labhoritari setup modhi ine kudzvanywa kwakanaka mukamuri yebuffer
Iyo PCR laboratory setup dhayagiramu yakapihwa muFigure A uye Figure B inofanirwa kuve yakanyanya kunaka yekuseta modhi, uye rabhoritari ine mamiriro inogona kureva iyi modhi yekugadzira.Kune akajairwa marabhoritari, zvinokurudzirwa kuti PCR amplification nzvimbo nenzvimbo yekuongorora chigadzirwa inogona kupatsanurwa, uye kuvhurwa kwevhavha kunofanirwa kudzikiswa zvakanyanya sezvinobvira munzvimbo yekugadzirira sampuli uye PCR amplification nzvimbo.Rangarira: Zvigadzirwa uye zvekuyedza zvekushandisa munzvimbo yekuongorora chigadzirwa zvinorambidzwa zvachose kuendeswa kunzvimbo yekugadzirira yemuenzaniso uye PCR nzvimbo yekusimudzira.
Kana marabhoritari ichingoona PCR uye kuzivikanwa, zvinokurudzirwa kushandisa fluorescent quantitative PCR pachinzvimbo chePCR yakajairika.
Fluorescence quantitative PCR yekuona mhedzisiro inogona kuunganidzwa uye kuongororwa nefluorescence masiginecha, saka hapana chikonzero chekuvhura chivharo che electrophoresis mushure mekuita, iyo inodzivirira kusvibiswa kwePCR chigadzirwa kunokonzerwa nekudonha kwemaitiro ekugadzira kugadzira aerosols.Kana iwe ukawedzera huwandu hwemavharo ekuvhura panguva yekurodha nhanho yegel electrophoresis, kusvibiswa kweaerosol kunogona kuitika.Inokurudzirwa kusimudzira kushandiswa kwehuwandu hwePCR uye zvishoma nezvishoma kutsiva qualitative PCR.
Iyo UNG anti-PCR chigadzirwa kusvibiswa system inoshandiswa kune PCR maitiro.
Iyo sisitimu inoshandisa dUTP pachinzvimbo che dTTP.Mushure mekuita kwePCR, zvese zvigadzirwa zvePCR (DNA zvidimbu) zvinosanganiswa nedUTP;muchikamu chinotevera chePCR reaction, iyo UNG enzyme yakawedzerwa kune system inoiswa pa 37 ° C kwemaminetsi mashanu pamberi pePCR, iyo inogona kunyatso shatisa zvese zvidimbu zveDNA zvine dUTP, uyezve kuita PCR reaction.Izvi zvinogona kubvisa zvachose kusvibiswa kweaerosol kunokonzerwa nePCR zvigadzirwa.Mhedzisiro inoratidzwa mumufananidzo uri pasi apa:
Ongorora: Kune yakananga PCR nhevedzano, unogona kusarudza akateedzana zvigadzirwa zveanti-PCR chigadzirwa chekusvibisa system yeForegene.Suggest
Kune marabhoritari anoita hukuru-hukuru genotyping bvunzo, zvinokurudzirwa zvakanyanya kushandisa iyo UNG anti-PCR chigadzirwa chetsvina system yekuyedza reagents mukuwedzera pakuvakwa kwemarabhoritari ane musoro.
Chiyeuchidzo: Kushandiswa kwegadziriro iyi hakugone kubvisa kusvibiswa kwechigadzirwa chePCR kwakatokonzerwa.Naizvozvo, iyo UNG system inofanirwa kushandiswa pakutanga kwebvunzo yakakodzera, uye iyo UNG system inofanirwa kushandiswa kukwidziridza PCR, kudzivirira kusvibiswa kwePCR zvigadzirwa Nhema dzakanaka.
Inokurudzirwa kushandisa Direct PCR-UNG system yeForegene paunenge uchiitisa bvunzo dzakakura, dzakadai se:
Plant Leaf Direct PCR Kit-UNG;
Dyara Mbeu Yakananga PCR Kit-UNG;
Animal Tissue Direct PCR Kit-UNG;
Mouse Muswe Wakananga PCR Kit-UNG;
Zebra Hove Yakananga PCR Kit-UNG.
Iyi nhevedzano yekiti kubva kuForegenehaigone kuita PCR yekuona nekukurumidza uye pamwero mukuru, asiwo zvinobudirira kudzivirira nekudzora PCR chigadzirwa kusvibiswa.
Nguva yekutumira: Mar-19-2021
