Nucleic acid kudhirowa inyanzvi yekutanga yekuongorora mamolecular biology.Kunyangwe ichigona kugona nekushandisa hunyanzvi uhu zvave zvakare chinhu chinodiwa kune vanozoedza vanotevera kuti vakwidziridze kuva masters ekuedzwa.
Zvinoenderana neakasiyana ekutsvagisa, vanoedza vanonyanya kukamurwa kuva anotevera marudzi matatu:
Mhuka huru, Chirimwa chikuru, Microbiology chikuru
Mhando yega yega yekuyedza inoenderera mberi nekukopa makopi kukudziridza seti yehunyanzvi hwega hwemasamples chaiwo.
Nhasi, mupepeti anoda kuunza mimwe michina uye mapuropu asingawanikwe kuburikidza negomba kune vanoedza.Navo, unogona kukurumidza kukwidziridza mumativi ese.Hakusi kurota kuva mwari anoedza.
Iyi nyaya ndeyekudyara nyanzvi dzekuyedza (vayedzeri vemamwe hunyanzvi vanogonawo kudzidza kubva mairi ~).
Ini ndinotenda kuti chizvarwa chega chega chevanoedza chichange chanzwa nezveiyo yekare chirimwa nucleic acid yekubvisa nzira dzakapfuudzwa kubva kuchizvarwa kuenda kune chimwe chizvarwa kubva kune avo vakavatangira.Iwo matatu mazita makuru muindasitiri yekuchera chirimwa.
Kunyange zvazvo vakomana vakuru vakanaka, vachine zvimwe zvinokanganisa zvisingadzivisiki.
Nzira yeTRIzol
Kazhinji inoshandiswa kuchenesa
RNA
●Muchetura uye unorema
●Iine genome kusvibiswa
CTAB nzira
Kazhinji inoshandiswa kuchenesa
genomeDNA
●Kutora nguva
● Zvakanyanya kuoma kubvisa CTAB nekuwacha kupfuura munyu, zvishoma zvakasara zvichave nemhedzisiro yakakura pakuita kwe enzyme.
SDS nzira
Kazhinji inoshandiswa kuchenesa
plasmid
● Haikodzeri zvirimwa zvine yakakwira polysaccharide
●Kusvibiswa kweprotein
Munharaunda ino yemazuva ano ine kaviri-speed update iterations, nzira dzechinyakare dzekushanda hadzichakwanisi kuzadzisa zvinodiwa nevanoedza, uye kuvandudzwa kwemidziyo kwave pedyo.
Foregene Nucleic Acid Extraction Kit yakaedzwa N nguva nevazhinji vevanoedza.Zviri nyore kushandisa, haishandise chero chepfu reagents, uye haiwedzere chero ma enzymes.Iyo nucleic acid inowanikwa ndeyekuchena kwepamusoro, iyo yakakosha pakuita kwezuva nezuva kudhirowa kwevanoedza vakanaka.
Foregene RNA Isolation kit
Pano, ini ndicharatidzawo nzira yakavanzika yekushanda kune vanoedza, iyo yekusarudza nzira yakakodzera yekuchera maererano nemhando yemiti (nzira yekuona rudzi rwechirimwa iri pamagumo echinyorwa!), Nokuti mamwe mapolysaccharides emiti uye polyphenols akakwirira mune zvinyorwa, uye nzira dzechinyakare dzinouraya.Hazvina kukwana kudzora.
Zvakanaka, sezvizvi ...
Iyo nzira yeTRIzol yakapfugama yakananga pasi pemasampuli ane yakakwira mapolysaccharides uye polyphenols semashizha ebhanana, mashizha eginkgo uye mashizha ekotoni.
Nechinangwa ichi, Foregene yakagadzira maturusi maviri anoshanda evanoedza.The chirimwa RNA extraction kit ine yakaderera polysaccharide uye polyphenol zvirimo uye yakakwira polysaccharide uye polyphenol zvirimo zvinogona kuuraya vavengi nenzira yakanangwa, zvekuti vanoedza havachatya zvirimwa zvakasiyana.Ingoshandura midziyo zvakananga!
Pamusoro pekudikanwa kwekusimudzira nzira yekubvisa, sampuli pre-processing mashandiro anodawo kukwidziridzwa.
Nekuda kwechimiro chakakosha chemasamples ezvirimwa, midzi nemadzinde akaoma ezvimwe zvirimwa zvinonetsa kukuya nekupwanya zvakaenzana.
Muindasitiri yekukuya, vanenge 80% yevanhu vanoshandisa liquid nitrogen pakukuya, nepo vasingasviki makumi maviri muzana vanoshandisa magetsi kukuya nekukuya bead oscillation.
Kunyangwe kukuya nitrogen yemvura kwakachipa uye kuri nyore kushandisa, inhamo kune vanoedza vane yakakura sampuli yekuda kukuya nguva yega yega uye kushanda nesimba!Kana iyo dhaka isina kucheneswa, inokonzeresa kusvibiswa pakati pemasampuli.
Mhedzisiro yekukuya kwemagetsi inosiyana kubva kumunhu kuenda kumunhu, uye maitiro ekushanda evanoedza achave nemhedzisiro yakakura pakutendeseka kweiyo nucleic acid, uye kuderera-kupisa kunofanirwa kana zvichidikanwa.
saka, kukuya bead oscillation inogona kutorwa seyazvino iri nyore uye inoshanda yekukuya michina.
Iwo akajairika oscillation modes anosanganisira vertical oscillation, horizontal reciprocating oscillation uye matatu-dimensional (horizontal oscillation).Nekudaro, Foregene's tissue crusher inotyora nepakati peiyo modhi iripo uye inotora nzira itsva ye4D oscillation kuita kuti kukuya kuwedzere yunifomu.
Sangana zvakasiyana-siyana zvemuenzaniso zvinodiwa, zviri nyore kushanda
Yakasarudzika 4D kupwanya tekinoroji, iyo yekupwanya inoshanda inowedzerwa ne20%
Muenzaniso wakavharirwa zvachose, hapana kusvibiswa-kusvibiswa
Kunyarara, ≤56 decibels
Enzanisa mhedzisiro yekuyedza kune wave ~
Test sample: 50mg mashizha egorosi (yakaderera zvirimo zvepolysaccharides uye polyphenols)
Bvisa nucleic acid mhando: RNA
Nhamba yenzvimbo: 5μl
Iyo kit yekubvisa inoshandiswa ndeye Foregene Plant Yese RNA Extraction Kit (RE-05011)
Kuenzanisa data uye electrophoresis girafu, zvinogona kuonekwa kuti kusungirirwa uye kuchena kweRNA yakabviswa mushure mekukuya kwemuchina kwakakwirira kudarika iyo yemvura nitrogen inokuya.Panguva imwecheteyo, nekuti muchina wekukuya unogona kutyora sampuli zvakanyanya, iyo diki RNA inogona kutorwa zvirinani uye kuvimbika kweRNA kunogona kuchengetedzwa.
Muenzaniso wekuedza: 50mg ginkgo mashizha (yakakwirira polysaccharide uye polyphenol zvirimo)
Bvisa nucleic acid mhando: RNA
Nhamba yenzvimbo: 5μl
Kushandisa Foregene Polysaccharide Polyphenol Plant Total RNA Extraction Kit (RE-05021)
Tarisa uone boka iri remasamples ane zvakakwirira zvemukati zvepolysaccharides uye polyphenols.Mhedzisiro yacho inotonyanya kuoneka.Nekuchena kwakafanana, kuwanda kwemuchina-kukuya RNA kungangoita kashanu kukuya kwenitrogen yemvura, uye RNA diki ichiri kuoneka zvisingaite ~
Kana iwe uchinzwa kuti hapana chimwe chezvishandiso chiri pamusoro chiri kukurumidza zvakakwana, saka mupepeti anofanira kuuya neakanyanya midziyo!
Dyara Direct PCR yakatevedzana
Chinhu chakasiyana chemidziyo iyi ndechekuti mashizha emuti kana mbeu haidi kuvhuniwa uye kuputsika, uye inokandwa zvakananga mu EP chubhu ye lysis, uye musanganiswa we lysis unogona kushandiswa se template yePCR reaction.Zviri nyore sedanho rimwe nekukurumidza!Panguva imwecheteyo, iyo yakananga PCR yakatevedzana yeForegene Plants yakashongedzerwa neUNG anti-kusvibisa system, kuitira kuti vanoedza vasazonetseka nezve kusvibiswa kweaerosol murabhoritari uye vanogona kutora kuyedza nyore.
Mazano ekutonga:
Maitiro ekutonga kana iri polysaccharide polyphenol chirimwa
Tora inenge 20mg tissue block uye uise mu 1.5ml centrifuge chubhu yakachena, wedzera 200ul 200mM NaOH mhinduro, isa pa 95 ℃ ye 10min, cherechedza kuchinja kwemavara mushure me lysis.
1. Kana ruvara rwe lysate rwakasvibirira kana kuti chiedza chero, zvinoreva kuti zvinyorwa zvetishu polyphenols zvakaderera, uye zvinokurudzirwa kushandisa Foregene Plant series.
2. Kana lysate iri brownish-yellow kana brownish-tsvuku, zvinoreva kuti tissue polyphenol content yakakwira, uye zvinokurudzirwa kushandisa Foregene Polysaccharide Polyphenol Plant Plus Series.
Nguva yekutumira: Aug-20-2021
