Viral DNA & RNA Isolation Kit Viral DNA uye RNA Extraction Kuchenesa Kugadzirira Kits
Zvinotsanangurwa
50 Preps, 200 Preps
Viral RNA Nucleic acid Kucheneswa Kubvisa Isolation Kit inoshandisa spin column uye fomula yakagadziriswa neForegene, iyo inogona kunyatso bvisa yakakwira-kuchena uye yemhando yepamusoro hutachiona RNA kubva kumasampuli akadai seplasma, serum, sero-isina masero emuviri, uye sero tsika supernatant.Iyo kit inonyanya kuwedzera Linear Acrylamide, iyo inogona kutora nyore zvidiki zveRNA kubva kumasampuli.RNA-Chete Column inogona kusunga RNA zvine mutsindo.Iyo kit inogona kugadzirisa nhamba huru yemasampuli panguva imwe chete.
Iyo yese kit haina RNase, saka iyo yakacheneswa RNA haizodzikisirwe.Buffer viRW1 uye Buffer viRW2 inogona kuve nechokwadi chekuti yakawanikwa hutachiona nucleic acid isina mapuroteni, nuclease kana kumwe kusvibiswa, iyo inogona kushandiswa zvakananga kuzasi kwema molecular biology kuyedza.
Kit zvikamu
| Linear Acrylamide |
| Buffer DRL |
| Buffer RW1, Buffer RW2 |
| RNase-Yemahara ddH2O |
| DNA/RNA Column |
| Mirayiridzo |
Zvimiro&zvakanakira
■ Kushanda pakamuri tembiricha (15-25 ℃) mukati mese muitiro, pasina kugeza nechando uye yakaderera tembiricha centrifugation.
■ Yakazara kit RNase-Yemahara, hapana chikonzero chekunetseka nezveRNA kudzikira.
■ High nucleic acid goho: DNA/RNA-chete Column uye yakasarudzika formula inogona kunyatsochenesa DNA neRNA.
■ Huru sampuli yekugadzirisa simba: kusvika ku200μl samples inogona kugadziriswa nguva imwe neimwe.
■ Kukurumidza kumhanya: nyore kushanda uye inogona kupedzwa mukati memaminitsi makumi maviri.
■ Kuchengetedza: hapana organic reagent inodiwa.
■ Hunhu hwepamusoro: Zvimedu zveRNA zvakacheneswa ndezvekuchena kwepamusoro, hazvina mapuroteni nehumwe tsvina, uye zvinokwanisa kusangana nemhando dzakasiyana dzekuedza dzepasi.
Kit application
Inokodzera kubviswa uye kucheneswa kwehutachiona nucleic acid mumasampuli akadai seplasma, serum, sero-isina mvura yemuviri uye sero tsika supernatant.
Kuyerera kwebasa
Mufananidzo
Storage uye Sherufu upenyu
∎ Kiti iyi inogona kuchengetwa kwemwedzi makumi maviri nemana pasi pemamiriro akaoma patembiricha yemumba (15-25℃);kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa mu2–8℃.
■ Linear Acrylamide mhinduro inogona kuchengetwa pakamuri tembiricha kwemazuva 7;mushure mekugamuchira kit, ndapota ibudise uye uichengete pa -20 ° C.
■ Mushure mekuwedzera Linear Acrylamide kuBuffer DRL, inogona kuchengetwa pa 2-8 ° C kusvika ku48h.Ndapota shandisa mhinduro yakagadzirwa.
Dambudziko rekuongorora Guide
Izvi zvinotevera kuongororwa kwezvinetso zvinogona kusangana mukubviswa kwehutachiona hweDNA / RNA, uchitarisira kubatsira kune zvaunoedza.Pamusoro pezvo, kune mamwe matambudziko ekuyedza kana ehunyanzvi kunze kwemirairo yekushanda uye kuongororwa kwedambudziko, isu takatsaurira tekinoroji rutsigiro kuti ikubatsire.Kana uine chero zvaunoda, tapota taura nesu: 028-83360257 kana E-mail:
Tech@foregene.com.
Hapana nucleic acid inotorwa kana yakaderera nucleic acid goho
Pane kazhinji zvinhu zvakawanda zvinokanganisa kugona kudzoreredza, senge: sampuli nucleic acid yemukati, nzira yekushandisa, elution vhoriyamu, nezvimwe.
Kuongororwa kwezvikonzero zvinowanzoitika:
1. Bhati yechando kana kutonhora kwakadzika (4 ° C) centrifugation yakaitwa panguva yekuita.
Zano: Shanda pane tembiricha yekamuri (15-25 ° C) mukati mese muitiro, usaite ice yekugezesa uye yakaderera tembiricha centrifugation.
2. Muenzaniso wakachengetwa zvisina kunaka kana sampuli yakachengetwa kwenguva yakareba.
Kurudziro: Chengetedza samples pa -80 ° C uye usadzore kutonhora nekunyunguduka;edza kushandisa zvichangobva kuunganidzwa sampuli kuti nucleic acid kutorwa.
3. Kusakwana sampuli lysis.
Kurudziro: Tapota onai kuti sampuli uye lysis kushanda mhinduro yakanyatsosanganiswa uye incubated pakamuri tembiricha (15-25 ° C) kwemaminitsi gumi.
4. Kuwedzera zvisirizvo kweeluent.
Kurudziro: Ita shuwa kuti RNase-Yemahara ddH2O inowedzerwa inodzika kusvika pakati pechenese column membrane, uye usadonhedze pakona yekuchenesa mhete.
5. Vhoriyamu chaiyo yeabsolute ethanol haina kuwedzerwa kuBuffer RW2.
Zano: Ndokumbira uteedzere mirairo, wedzera huwandu chaihwo hweabsolute ethanol kuBuffer RW2 uye sanganisa zvakanaka usati washandisa kit.
6. Sample vhoriyamu isina kufanira.
Zano: 200µl yesampuli inogadziridzwa kune yega yega 500µl yeBuffer DRL.Yakawandisa sampuli yekugadzirisa inozokonzera yakaderera nucleic acid yekubvisa goho.
7. Vhoriyamu yekuvhota isina kufanira kana kusakwana.
Kurudziro: Vhoriyamu yakasarudzika yekoramu yekuchenesa ndeye 30-50μl;kana iyo elution mhedzisiro isiri inogutsa, zvinokurudzirwa kuwedzera nguva pakamuri tembiricha mushure mekuwedzera preheated RNase-Mahara ddH2O, senge 5-10min.
8. Ethanol inoramba iri pambiru mushure mekugeza neBuffer RW2.
Zano: Kana ethanol ikasara mushure mekuisa centrifugation neBuffer RW2 kwemaminetsi maviri, iyo column inogona kuiswa pakamuri tembiricha kwemaminetsi mashanu mushure mekuita centrifugation kuti ibvise zvachose yakasara ethanol.
Iyo yakacheneswa nucleic acid yakasvibiswa
Hunhu hwekucheneswa nucleic acid hunoenderana nekuchengetedza sampuli, kusvibiswa kweRNase, kushanda uye zvimwe zvinhu.Kuongororwa kwezvikonzero zvinowanzoitika:
1. Masampuli akaunganidzwa haana kuchengetwa nenguva.
Zvaungaita: Kana sampuli ikasashandiswa munguva mushure mekuunganidza, tapota chengeta pa -80 ° C pakupisa kwakaderera pakarepo.Kune RNA kutorwa, edza kushandisa masampuli achangobva kuunganidzwa.
2. Unganidza sampuli uye omesa uye unyunguduke kakawanda.
Zano: Dzivisa kutonhora nekunyunguduka (kwete kanopfuura kamwe) panguva yekuunganidza uye kuchengetwa kwemasampuli, zvikasadaro iyo nucleic acid goho ichaderedzwa.
3. RNase inounzwa mukamuri yekuvhiya kana magurovhosi anoraswa, masks, nezvimwe hazvina kupfekwa.
Kurudziro: Maedzo ekubvisa eRNA anoitwa zvakanyanya mukamuri yekuvhiya yeRNA, uye tafura yemurabhoritari inofanirwa kucheneswa kusati kwaitwa.
Pfeka magurovhosi anoraswa uye masiki panguva yekuyedza kudzivirira kusvibiswa kweRNA kunokonzerwa nekuunzwa kweRNase zvakanyanya.
4. Iyo reagent yakasvibiswa neRNase panguva yekushandiswa.
Kurudziro: Tsiva neViral DNA/RNA Isolation Kit yezviyedzo zvine hukama.
5. Machubhu ecentrifuge nemapipetti anoshandiswa paRNA manipulation akasvibiswa neRNase.
Zvaungaita: Ita shuwa kuti machubhu ecentrifuge, matipi epipette, mapipetti, nezvimwewo zvinoshandiswa pakubvisa RNA zvese zviri RNase-Mahara.
Yakanatswa nucleic acid inokanganisa kuyedza kwepasi
DNA neRNA zvakacheneswa nekoramu yekuchenesa, kana iyo ion yemunyu uye mapuroteni akanyanya, zvinokanganisa kuyedza kudzika, senge: PCR amplification, reverse transcription, nezvimwe.
1. Iyo yakaraswa DNA neRNA zvine maion emunyu asara.
Zano: Ita shuwa kuti vhoriyamu chaiyo yeabsolute ethanol inowedzerwa kuBuffer RW2, uye geza koramu yekuchenesa kaviri pakumhanya kwecentrifugation inotsanangurwa mumirairo yekushandisa;Ita centrifugation kuderedza kusvibiswa kwemunyu.
2. DNA neRNA yakaraswa ine zvisaririra zve ethanol.
Mazano: Mushure mekusimbisa kushambidza neBuffer RW2, ita isina chinhu tube centrifugation pa centrifugation speed mumirairo yekushanda;kana kuchine ethanol zvakasara, unogona centrifuge chubhu isina chinhu uye woiisa pakamuri tembiricha kwemaminitsi 5 kubvisa ethanol zvakasara kusvika pamwero mukuru.
Mirayiridzo yezvinyorwa:
Viral DNA & RNA Isolation Kit Instruction Manual
