Serum(Plasma) miRNA Isolation Kit
Kit Description:
◆Yakazara set kit RNase-Yemahara, hapana chikonzero chekunetseka nezveRNA kudzikira.
◆DNA-Kuchenesa Column inosungirira kuDNA, kuitira kuti kit ibvise genomic DNA kusvibiswa pasina kuwedzera imwe DNase.
◆Yakakwira RNA goho: RNA-chete Column uye yakasarudzika fomula inogona kunyatso chenesa RNA.
◆Kukurumidza kumhanya: Kushanda kuri nyore uye kunogona kupedzwa mukati memaminitsi makumi matatu.
◆Chengetedzo: Hapana organic reagent kutorwa kunodiwa.
◆Hunhu hwepamusoro: Zvimedu zvakabviswa zveRNA zvine kuchena kwakanyanya, zvisina mapuroteni uye kumwe kusvibiswa, uye zvinogona kusangana nemhando dzakasiyana dzezasi dzekuyedza maapplication.
Tsanangudzo
Iyi kit inoshandisa spin column uye fomula yakagadziridzwa nekambani yedu, iyo inogona kunyatsoburitsa zvimedu zvidiki zvemiRNA zvisingasviki zana kubva muserum uye plasma.Iyo kit inopa DNA-Kuchenesa Column, iyo inogona nyore kuparadzanisa supernatant uye tishu lysate uye adsorb uye kubvisa DNA mumuenzaniso, iyo iri nyore uye inochengetedza nguva;RNA-chete Column inogona kusunga RNA zvine mutsindo, uye neyakasarudzika fomula, inogona kushandiswa panguva imwe chete.Gadzira nhamba huru yemasampuli.
Iyo yese sisitimu ndeye RNase-Yemahara, kuitira kuti yakanatswa miRNA irege kuderedzwa;Buffer miBRW1 uye Buffer miBRW2 buffer washing system inoita kuti RNA iwanikwe isina protein, DNA, ion, uye organic compound kusvibiswa.
Zvinotsanangurwa
5T, 50T, 100T, 200T
Kit zvikamu
| Serum/Plasma miRNA Isolation Kit | ||||
| Kit zviri mukati | RE-0111T | RE-01111 | RE-01112 | RE-01114 |
| 5 T | 50 T | 100T | 200T | |
| Buffer miBRL1* | 2ml | 20ml | 40ml | 80ml |
| Buffer miBRL2* | 1.2ml | 12ml | 24ml | 48ml |
| Buffer miBRW1 | 2ml | 20ml | 40ml | 80ml |
| Buffer miBRW2 | 2.4ml | 24ml | 48ml | 96ml |
| RNase-Yemahara ddH2O | 1.7ml | 10ml | 20ml | 40ml |
| RNA-chete Column | 5 zvimedu | 50zvimedu | 100zvimedu | 200zvimedu |
| DNA-Kuchenesa Column | 5 zvimedu | 50zvimedu | 100zvimedu | 200zvimedu |
| IFU | 1 chidimbu | 1 chidimbu | 1 chidimbu | 1 chidimbu |
Zvimiro&zvakanakira
◆Yakazara set kit RNase-Yemahara, hapana chikonzero chekunetseka nezveRNA kudzikira.
◆DNA-Kuchenesa Column inosungirira kuDNA, kuitira kuti kit ibvise genomic DNA kusvibiswa pasina kuwedzera imwe DNase.
◆Yakakwira RNA goho: RNA-chete Column uye yakasarudzika fomula inogona kunyatso chenesa RNA.
◆Kukurumidza kumhanya: Kushanda kuri nyore uye kunogona kupedzwa mukati memaminitsi makumi matatu.
◆Chengetedzo: Hapana organic reagent kutorwa kunodiwa.
◆Hunhu hwepamusoro: Zvimedu zvakabviswa zveRNA zvine kuchena kwakanyanya, zvisina mapuroteni uye kumwe kusvibiswa, uye zvinogona kusangana nemhando dzakasiyana dzezasi dzekuyedza maapplication.
Kit parameters
Kit Application
Inokodzera kubviswa uye kucheneswa kwe miRNA kubva kumhuka uye serum yevanhu uye plasma.
miRNA Application
Iyo miRNA yakacheneswa neSerum/Plasma miRNA Isolation Kit inogona kushandiswa mumhando dzakasiyana dzekudzika mamorekuru kuyedza, senge: RT-PCR, Real Time RT-PCR, Northern Blot, chip analysis, nezvimwe.
Kuyerera kwebasa
Kuchengetedza mamiriro
Kiti iyi inogona kuchengetwa kwemwedzi gumi nemiviri pasi pemamiriro akaoma patembiricha yekamuri(15-25°C).Ikana inoda kuchengetwa kwenguva yakareba,Ndapotakuchengetwa pa2-8°C.
