SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)
Kit Description:
◮ Yakananga PCR:Hapana kudikanwa kweiyo nucleic acid yekubvisa kit uye System, yakapedzwa 96 specimen muawa imwe kana zvakadaro.
◮ Kudiwa kwemidziyo yakaderera uye kushandiswa kwakanyanya kuchinjika:Inongoda Real-Nguva PCR system.
◮ LoD uye yakanyanya kunzwa:Detection senitivity inogona kusvika padanho rakaderera se500 makopi/ml.
Chinangwa Kushandiswa
Nheyo yekuedza
Iyi yekutanga uye seti yekuferefeta yakagadzirirwa kucherechedzwa kweakachengetwa akateedzana eSARS-CoV-2 (ORF1ab gene, N gene uye E gene).Iyo kit ine Yemukati Kudzora iyo inoshandiswa kuongorora mhando yemuenzaniso.Real-Nguva PCR tekinoroji inoshandisa polymerase chain reaction (PCR) yekukwidziridza yezvakananga mateedzana uye kunanga kwakananga maprobes ekuonekwa kweiyo RNA yakasimudzwa.Iwo maprobe akanyorwa nefluorescent reporter uye quencher dhayi.
Quick Details
◮Zvinodiwa:48 Rxns/kiti, 96 Rxns/kiti
◮ Zvakananga:96.72%
Sensitivity:97.92%
Zvikamu
| Aihwa. | Chikamu | Mari | Main ingredient | |||
| 48 Rxns | 96 Rxns | |||||
| 1 | Nucleic acid kusunungurwamumiririri | 1.5 mL / chubhu | 1 chubhu | 1.5 mL / chubhu | 1 chubhu | Surfactant |
| 2 | RNA inodzivirira | 27 μL/chubhu | 1 chubhu | 53 μL/chubhu | 1 chubhu | RNase inhibitor |
| 3 | SARS-CoV-2reaction solution | 800 μL / chubhu | 1 chubhu | 1600 μL/chubhu | 1 chubhu | Primer, probe, reaction buffer, dNTP |
| 4 | SARS-CoV-2enzyme musanganiswa | 80 μL/chubhu | 1 chubhu | 160 μL/chubhu | 1 chubhu | Kupisa kutanga Taq enzyme,M-MLV enzyme |
| 5 | SARS-CoV-2kudzora kwakanaka | 100 μL / chubhu | 1 chubhu | 100 μL / chubhu | 1 chubhu | Recombinant plasmid ine chinangwachikamu, RNA |
| 6 | SARS-CoV-2kudzora kwakashata | 1200 μL / chubhu | 1 chubhu | 1200 μL / chubhu | 1 chubhu | TE buffer |
Zvimiro&zvakanakira
Kushandisa sei
Nzira 1: Yakananga PCR nzira
Nzira 2: Viral RNA Isolation + PCR
Nzira 1: Yakananga PCR nzira uchishandisaSample Release Agent
Nzira yekuona
Cold Chain Kutakura uye Kuchengeta
Yakavharwa kubva kuchiedza uye yakachengetwa pa -20±5 ℃;
Sherufu hupenyu
1 gore
Kuraira Ruzivo
