RT-qPCR Easyᵀᴹ (Imwe Danho)-Taqman
Kit Description:
- Poshi-nhanho kit inogonesa reverse transcription uye PCR kuti iitwe mune imwechete chubhu, inongoda kuwedzera template RNA, chaiyo PCR primers uye RNase-Mahara ddH.2O.
-Chaiyo-nguva yehuwandu kuongororwa kwehutachiona RNA kana kutsvaga RNA inogona kuitwa nekukurumidza uye nemazvo.
-Iyo kit inoshandisa yakasarudzika Foregene reverse transcription reagent uye Foregene HotStar Taq DNA Polymerase yakasanganiswa neyakasarudzika maitiro sisitimu kuti inyatso kunatsiridza kugona kwekusimudzira uye kujeka kwemaitiro.
- Iyo yakagadziridzwa maitiro system inoita kuti maitiro ave nepamusoro ekuona senitivity, kusimba kwekushisa kwekushisa, uye kushivirira kuri nani.
- RT-qPCR NyoreTM(Imwe Danho) -Taqman kit inouya neROX yemukati referensi dhayi, iyo inogona kushandiswa kubvisa chiratidzo chekumashure uye zvikanganiso zvechiratidzo pakati pematsime, izvo zviri nyore kune vashandisi vekupedzisira kushandisa mumhando dzakasiyana dzehuwandu hwePCR zviridzwa.
Tsanangudzo
Foregene Imwe-Nhanho RT-PCR Zvigadzirwa zvakapusa zvakatevedzana zvinoona maitiro akabatanidzwa kubva kuRNA kuenda kune yakapetwa kaviri DNA, kureva, reverse transcript uye PCR kukwidziridzwa kunopedzwa mune imwechete reaction centrifuge chubhu uye yakafanana maitiro system, nokudaro matanho ekuedza akarerutswa, chirongwa chekuyedza chakagadziridzwa, uye kugona kwekuyedza kwakavandudzwa.
RT-qPCR NyoreTM (PoshiDanho)-Taqmanuchishandisa Foregene HotStar Taq DNA Polymerase, iyo yakagadziridzwa Taqman qPCR sisitimu inogona kukurumidza uye chaizvo kuita Chaiyo Nguva RT-qPCR yehuwandu hwekuona yekutsvaga matemplate eRNA.
Zvinotsanangurwa
100 Rxns (20μl system)
500 Rxns (20μl system)
Kit zvikamu
| RT-qPCR EasyTM (Imwe Danho)-Taqman | ||
| Kit zvikamu | RT-02131 | RT-02132 |
| 100Rxns (20μl system) | 500 Rxns (20μl system) | |
| 2 × RT-qPCR NyoreTMMix-Taqman | 1ml | 1.7ml×3 |
| 20 × ROX Reference Dye | 100μl | 0.5ml |
| RNase-Yemahara ddH2O | 1.7ml | 1.7ml×3 |
| Murayiridzo Wekushandisa | 1 | 1 |
Zvimiro&zvakanakira
- Imwe-nhanho kit inogonesa reverse transcription uye PCR kuti iitwe muhubhu imwechete, inongoda kuwedzera template RNA, chaiyo PCR primers uye RNase-Mahara ddH.2O.
-Chaiyo-nguva yehuwandu kuongororwa kwehutachiona RNA kana kutsvaga RNA inogona kuitwa nekukurumidza uye nemazvo.
-Iyo kit inoshandisa yakasarudzika Foregene reverse transcription reagent uye Foregene HotStar Taq DNA Polymerase yakasanganiswa neyakasarudzika maitiro sisitimu kuti inyatso kunatsiridza kugona kwekusimudzira uye kujeka kwemaitiro.
- Iyo yakagadziridzwa maitiro system inoita kuti maitiro ave nepamusoro ekuona senitivity, kusimba kwekushisa kwekushisa, uye kushivirira kuri nani.
- RT-qPCR NyoreTM(Imwe Danho) -Taqman kit inouya neROX yemukati referensi dhayi, iyo inogona kushandiswa kubvisa chiratidzo chekumashure uye zvikanganiso zvechiratidzo pakati pematsime, izvo zviri nyore kune vashandisi vekupedzisira kushandisa mumhando dzakasiyana dzehuwandu hwePCR zviridzwa.
Kit parameters
Kit application
-Iyi kit inoshandiswa kukurumidza uye kwakaringana kuona kwekutsvaga maRNA akadai seRNA mavhairasi.
-Iyo inonyanya kukodzera kutariswa nekukurumidza kweRNA matemplate ane yakakwira GC zvemukati kana yakaoma yechipiri chimiro.
Workflow
Kuchengetedza mamiriro
1.Transportationmamiriro
Iyo yese maitiro ekudzika-tembiricha echando bhokisi chekufambisa, kuve nechokwadi chekuti kit iri munzvimbo ye <4 ℃.
2.Storage mamiriro
Iyo kit inochengetwa pa -20 ℃.Chengetedza chigadzirwa mufiriji yekushisa nguva dzose-20 ℃ pakarepo mushure mekugamuchira.Kana mamiriro ekuchengetedza akakodzera, chigadzirwahaizodzikisi chero mashandiro panguva ye1-gore yechokwadi nguva.
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