RT-qPCR Easyᵀᴹ (Imwe Danho) -SYBR Green I
Kit Description:
◮Imwe-nhanho kit inoita reverse transcription uye qPCR maitiro maviri muchubhu imwechete, inongoda kuwedzera template RNA, chaiyo PCR primers uye RNase-Mahara ddH.2O.
◮Iyo kit inogona nekukurumidza uye zvine hungwaru kuongorora hutachiona RNA kana kutsvaga RNA.
◮Iyo kit inoshandisa yakasarudzika yeForegene reverse transcription reagent uye Foregene HotStar Taq DNA Polymerase yakasanganiswa neyakasarudzika maitiro sisitimu kuti inyatso kunatsiridza kugona kwekusimudzira uye kujeka kwemaitiro.
◮Iyo optimized reaction system inoita kuti mhinduro ive nepamusoro yekuona senitivity, kusimba kwekupisa kwekushisa, uye kushivirira kuri nani.
◮RT-qPCR NyoreTM(Imwe Danho) -SYBR Green I kit inouya neROX yemukati referensi dhayi, iyo inogona kushandiswa kubvisa chiratidzo chekumashure uye zvikanganiso zvechiratidzo pakati pematsime, izvo zviri nyore kuti vatengi vashandise mumhando dzakasiyana dzehuwandu hwePCR zviridzwa.
Tsanangudzo
RT-qPCR NyoreTM (Imwe Danho) -SYBR Green I inoshandisa yakasarudzika sisitimu kusanganisa zvinobudirira RT uye Real Time PCR maitiro, ayo anogona kukurumidza kupedzisa kuwanda kwekuona kwemajini.Chigadzirwa chacho chine kushivirira kwakasimba, hukuru hwepamusoro uye kugadzikana.Chigadzirwa chacho chine yakasarudzika yekambani Foregene HotStar Taq DNA Polymerase, iyo ine zvakanakira kukurumidza kukwidziridza (2Kb/min), yakakwirira amplification kunyatsoshanda, kwakasimba kwakasimba kukwirisa, uye yakaderera mwero wekukanganisa uchienzaniswa neakajairwa maTaq enzymes.Inoshandiswa pano yeReal Time RT-PCR kudzikisa kusiri-chaiyo amplification uye kugadzirisa huchokwadi hwePCR.
Zvinotsanangurwa
100 T (20μl system)
500 T (20μl system)
Kit zvikamu
| RT-qPCR NyoreTM(Imwe Danho) -SYBR Green I | ||
| Kit Zviri mukati | RT-02111 | RT-02112 |
| 100T (20μl system) | 500T (20μl system) | |
| 2 × RT-qPCR NyoreTM Mix-SYBR | 1ml | 1.7ml×3 |
| 50 × ROX Reference Dye | 200μl | 1ml |
| RNase-Yemahara ddH2O | 1.7ml | 1.7ml×3 |
| Bhuku reMirayiridzo | 1chidimbu | 1 chidimbu |
Zvimiro&zvakanakira
- Imwe-nhanho kit inoita reverse transcription uye qPCR maviri maitiro muchubhu imwechete, inongoda kuwedzera template RNA, chaiyo PCR primers uye RNase-Mahara ddH.2O.
- Iyo kit inogona nekukurumidza uye zvine hungwaru kuongorora hutachiona RNA kana kutsvaga RNA.
-Iyo kit inoshandisa yakasarudzika Foregene reverse transcription reagent uye Foregene HotStar Taq DNA Polymerase yakasanganiswa neyakasarudzika maitiro sisitimu kuti inyatso kunatsiridza kugona kwekusimudzira uye kujeka kwemaitiro.
- Iyo yakagadziridzwa maitiro system inoita kuti maitiro ave nepamusoro ekuona senitivity, kusimba kwekushisa kwekushisa, uye kushivirira kuri nani.
- RT-qPCR NyoreTM(Imwe Danho) -SYBR Green I kit inouya neROX yemukati referensi dhayi, iyo inogona kushandiswa kubvisa chiratidzo chekumashure uye zvikanganiso zvechiratidzo pakati pematsime, izvo zviri nyore kuti vatengi vashandise mumhando dzakasiyana dzehuwandu hwePCR zviridzwa.
Workflow
Kuchengetedza mamiriro
1.Transportationmamiriro
Iyo yese maitiro ekudzika-tembiricha echando bhokisi chekufambisa, kuve nechokwadi chekuti kit iri munzvimbo ye <4 ℃.
2.Storage mamiriro
Iyo kit inochengetwa pa -20 ℃.Chengetedza chigadzirwa mufiriji yekushisa nguva dzose-20 ℃ pakarepo mushure mekugamuchira.Kana mamiriro ekuchengetedza akakodzera, chigadzirwahaizodzikisi chero mashandiro panguva ye1-gore yechokwadi nguva.
