QuickEasyᵀᴹ Real Time PCR Kit-Taqman
Kit Description:
◮Iyo yakasarudzika PCR optimization system inoita 2 × QuickEasyTMReal PCR Mix-Taqman inoenderana zvakanyanya.
◮Kupisa-kutanga Foregene HS Taq Polymerase ine yakakwirira kukwidziridza kunyatsoita, yakakwirira amplification senitivity, uye yakakwirira amplification chaiyo.
◮2 × QuickEasyTMReal PCR Mix -Taqman inoshandisa yakasarudzika sisitimu yakagadziridzwa neForegene kuvandudza kunzwisiswa uye kujeka kwekutevedzana-chaiyo probe yekuona, iyo inogona kushandiswa genotyping uye kukopa nhamba kusiyanisa kusarudza uye kuwana mhedzisiro chaiyo.
◮Ichi chigadzirwa chinouya neROX yemukati referensi dhayi, iyo inogona kushandiswa kubvisa chiratidzo chekumashure uye chiratidzo chikanganiso pakati pematsime, ayo ari nyore kuti vatengi vashandise mumhando dzakasiyana dzehuwandu hwePCR zviridzwa.
Tsanangudzo
Iyo 2 × QuickEasyTMReal PCR Mix-Taqman yakapihwa neiyo QuickEasyTMReal Time PCR Kit-Taqman kit ichizvarwa chitsva master musanganiswa system yakagadzirirwa Real Time PCR amplification reaction uchishandisa chaiyo fluorescent probes.Yayo musimboti Foregene HS Taq DNA Polymerase yakavakirwa paAntibody gadziriso, inoshandiswa pamwe chete neForegene yakawedzera optimized PCR system, ine hunyanzvi hwepamusoro uye kugona kwekusimudzira.Kuenzaniswa neyakajairwa PCR musanganiswa, ine simba rekusimudzira kugona uye yakaderera mismatch mwero.Inogona kushandiswa mufluorescence quantitative PCR kuita kuderedza kusiri-chaiyo amplification uye kunatsiridza huchokwadi hwePCR.Inogona kuvandudza zvakanyanya chigadzirwa chakanangana nekuita senitivity.Panguva imwecheteyo, ROX yakapihwa seyemukati referensi dhayi.
Iyo QuickEasyTMReal Time PCR Kit-Taqman kit ine yakafara chiyero chekushandisa kune huwandu hwetemplate, uye inogona kuwana yakanaka yakajairwa curve munzvimbo dzakasiyana dzehuwandu, uye inokwanisa kunyatso kuverenga uye kuona magene ekunangwa nekudzokorora kwakanaka uye kuvimba kwakanyanya.
Zvinotsanangurwa
200 Preps,500 Preps,1000 Preps,2000 Preps
Kit zvikamu
| QuickEasyTMReal Time PCR Kit-Taqman(20μlsystem) | ||||
| Kit zviri mukati | QP-01121 | QP-01122 | QP-01123 | QP-01124 |
| 200 Preps | 500 Preps | 1000 Preps | 2000 Preps | |
| 2×QuickEasyTM PCR chaiyo Mix-Taqman | 1 mL×2 | 1.7 mL×3 | 1.7 mL×6 | 1.7 mL×12 |
| 20×ROX Reference Dye | 200μL | 0.5 mL | 1 mL | 1 mL×2 |
| DNase-Yemahara ddH2O | 1.7 mL | 1.7 mL×2 | 10 mL | 20 mL |
| IFU | 1chidimbu | 1chidimbu | 1chidimbu | 1chidimbu |
Zvimiro&zvakanakira
■ Iyo yakasarudzika PCR optimization system inoita 2×QuickEasyTMReal PCR Mix-Taqman inoenderana zvakanyanya.
■ Kupisa-kutanga Foregene HS Taq Polymerase ine kukwirira kwekusimudzira, kunzwisiswa kwepamusoro-soro, uye kukwidziridzwa kwepamusoro.
■ 2× QuickEasyTM Real PCR Mix -Taqman inoshandisa yakasarudzika sisitimu yakagadziridzwa neForegene kuvandudza kunzwisiswa uye kujeka kwekutevedzana-chaiyo probe yekuona, iyo inogona kushandiswa genotyping uye kukopa nhamba kusiyanisa kusarudza uye kuwana mhedzisiro chaiyo.
■ Ichi chigadzirwa chinouya neROX yemukati referensi dhayi, iyo inogona kushandiswa kubvisa chiratidzo kumashure uye chiratidzo chikanganiso pakati pematsime, izvo zviri nyore kuti vatengi vashandise mumhando dzakasiyana dzehuwandu hwePCR zviridzwa.
Kit application
■ Fluorescence quantitative PCR for template quantitative analysis;
■ Conventional PCR amplification;
∎ Inogona kushandiswa pakuonekwa kwe allele.
Storage uye Sherufu upenyu
Iyo kititi inofanira kuchengetwa pa -20 ° C murima;kana ikashandiswa kakawanda, inogonawo kuchengetwa pa4 ° C kwenguva pfupi (shandisa mukati memazuva gumi).
Real Time PCR primer dhizaini misimboti
Pamberi Primer uye Reverse Primer
Kune Real Time PCR, dhizaini yekutanga yakakosha.MaPrimers ane hukama nehunyanzvi uye kugona kwePCR amplification, uye inogona kugadzirwa ichitarisa kune anotevera misimboti:
Kureba kwekutanga: 18-30bp.
GC yemukati: 40-60%.
Tm kukosha: Primer dhizaini software, senge Primer 5, inogona kupa iyo Tm kukosha kweiyo primer.Iyo Tm tsika dzekumusoro uye pasi pemvura dzekutanga dzinofanira kunge dziri padyo sezvinobvira.The Tm calculation formula inogonawo kushandiswa: Tm = 4 °C (G + C) + 2 °C (A + T).Paunenge uchiita PCR, tembiricha iri pasi peiyo primer Tm kukosha kwe5 ° C inowanzo sarudzwa seye tembiricha yekudziya (iyo inowirirana kuwedzera kwekushisa kweanona inogona kuwedzera iyo chaiyo yePCR reaction).
Primers uye PCR zvigadzirwa:
Dhizaini primer PCR yekusimudzira chigadzirwa kureba ingangoita 100-150bp.
Dhizaini yekutanga munzvimbo yechipiri yemaitiro eiyo template inofanira kudziviswa zvakanyanya sezvinobvira.
Dzivisa kuumbwa kwe 2 kana anopfuura mabhesi anowirirana pakati pe 3′ migumo yekumusoro nekudzika kwemvura.
Primer 3′ terminal base haigone kuvepo ne3 yekuwedzera yakatevedzana G kana C.
Iwo maprimer pachawo haagone kuve neanopindirana zvimiro, zvikasadaro chimiro chebvudzi chinoumbwa, chinokanganisa PCR amplification.
ATCG inofanirwa kugovaniswa zvakaenzana sezvinobvira mukutevedzana kwekutanga, uye 3 ′ terminal base inofanira kudzivirirwa seT.
Appendikisi 1: Cell Direct RT-qPCR Kit chikamu chekuwedzera paki
1.Sero Lysis Solution
|
| |||
| Kit zvikamu (24-tsime lysis system / tsime) | DRT-01011-A1 | DRT-01011-A2 | |
| 100 T | 500 T | ||
| ChikamuI | Buffer CL | 20 ml | 100 ml |
| Foregene Protease Plus II | 400 μl | 1 ml × 2 | |
| Buffer ST | 1 ml × 2 | 10 ml | |
| ChikamuII | DNA Eraser | 400 μl | 1 ml × 2 |
2.RT Mix
|
| |
| Kit zvikamu (20 μl reaction system) | DRT-01011-B1 |
| 200 T | |
| 5 × Yakananga RT Musanganiswa | 800 μl |
| RNase-Yemahara ddH2O | 1.7 ml × 2 |
|
| ||
| Kit zvikamu (20 μl reaction system) | DRT-01011-C1 | DRT-01011-C2 |
| 200 T | 1000 T | |
| 2 × Yakananga qPCR Musanganiswa-SYBR | 1 ml × 2 | 1.7 ml × 6 |
| 50 × ROX Reference Dye | 40 μl | 200 μl |
| RNase-Yemahara ddH2O | 1.7 ml | 10 ml |
-
Buccal Swab/FTA Kadhi DNA Isolation Kit Genomic ...
-
Dyara Mbeu (Polysaccharide Polyphenol yakapfuma, ine smal ...
-
Foregene DNA Identification System 38Y-C (Nyaya)
-
Zebra Hove Yakananga PCR Kit-UNG Yakananga PCR Lysis ...
-
Stool DNA Isolation Kit DNA Extraction Purifica...
-
MALT1 Dual Color Break Apart probe
