Dyara Yese RNA Isolation Plus Kit
Kit Description:
Kunyatso bvisa DNA uchishandisa DNA-Cleaning Column
Bvisa DNA pasina kuwedzera DNase
Zviri nyore - mashandiro ese anopedzwa patembiricha yekamuri
Kukurumidza-kushanda kunogona kupedzwa mumaminitsi makumi matatu
Yakachengeteka-hapana organic reagent inoshandiswa
Zvinotsanangurwa
50 Preps, 200 Preps
Iyo kit inoshandisa spin column uye fomula yakagadziridzwa neForegene, iyo inogona kunyatso kuburitsa yakakwira-kuchena uye yemhando yepamusoro RNA kubva kwakasiyana siyana matishu ane yakakwira polysaccharides kana polyphenols zvirimo.Inopa iyo DNA-Kuchenesa koramu inogona kubvisa nyore genomic DNA kubva kune supernatant uye tishu lysate.RNA-chete column inogona kunyatsosunga RNA.Iyo kiti inogona kugadzirisa nhamba huru yemasampuli panguva imwe chete.
Iyo yese system haina RNase, saka iyo yakacheneswa RNA haizodzikisirwe.Buffer PRW1 uye Buffer PRW2 inogona kuvimbisa kuti RNA inowanikwa haina kusvibiswa neprotein, DNA, ions, uye organic compounds.
Kit zvikamu
| Buffer PSL1, Buffer PS, Buffer PSL2 |
| Buffer PRW1, Buffer PRW2 |
| RNase-Yemahara ddH2O, DNA-Cleaning Column |
| RNA-Chete Column |
Zvimiro&zvakanakira
■ Kushanda pakamuri tembiricha (15-25 ℃) mukati mese muitiro, pasina kugeza nechando uye yakaderera tembiricha centrifugation.
■ Yakazara kit RNase-Yemahara, hapana chikonzero chekunetseka nezveRNA kudzikira.
∎ Inonyanya kukodzera kucheneswa kweRNA kubva kumiti yemichero yemapolysaccharides uye polyphenols.
∎ DNA-Cleaning Column inonyatso sunga kuDNA, kuitira kuti kit inogona kubvisa genomic DNA kusvibiswa pasina kuwedzera DNase.
■ Yakakwira RNA goho: RNA-chete Column uye yakasarudzika fomula inogona kunyatsochenesa RNA.
■ Kukurumidza kumhanya: nyore kushanda uye inogona kupedzwa mukati memaminitsi makumi matatu.
■ Kuchengetedza: hapana organic reagent inodiwa.
■ Hunhu hwepamusoro: Zvimedu zveRNA zvakacheneswa ndezvekuchena kwepamusoro, hazvina mapuroteni uye kumwe kusvibiswa, uye zvinokwanisa kusangana nemhando dzakasiyana dzekuedza dzepasi.
Product parameters
■ Zvishandiso zvepasi: yekutanga-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, nezvimwewo.
■ Muenzaniso: Zvitsva kana zvakaomeswa zvezvirimwa zvemapolysaccharides nemapolyphenols
■ Dosage: 50mg chirimwa tishu
■ Yakakura RNA inosunga simba rekuchenesa column: 80 μg
■ Elution volume: 50-200 μl
Kit application
Yakakodzera kubviswa uye kucheneswa kweRNA yakazara kubva kutsva kana chando matishu echirimwa samples (kunyanya nyowani yemashizha emuti wetishu) ine yakakwira polysaccharide uye polyphenol zvemukati.
Kuyerera kwebasa
Mufananidzo
Plant Yese RNA Isolation Kit Plus yakagadziriswa 50mg yemashizha matsva epolysaccharides uye polyphenols, uye 5% yakacheneswa RNA yakaedzwa ne electrophoresis.
1: Banana
2: Ginkgo
3: Donje
4: Damba
Storage uye Sherufu upenyu
Iyi kit inogona kuchengetwa kwemwedzi makumi maviri nemana pasi pemamiriro akaoma pane tembiricha yekamuri (15-25 ℃);kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa mu2–8℃.
Buffer PSL1 inogona kuiswa pa 4 ℃ kwemwedzi 1 mushure mekuwedzera β-mercaptoethanol (inokurudzirwa kuiwedzera panguva imwe chete yekuedza).
