Dyara shizha Direct PCR kit-UNG (isina Sampling Zvishandiso) Protocol Direct PCR kubva kuPlat Material
Tsanangudzo
Kubva pane Plant Leaf Direct PCR kit, dUTP inoshandiswa pachinzvimbo che dTTP, uye iyo UNG enzyme inogona kukanganisa template ine dUTP inowedzerwa.Nenzira iyi, iyo chaiyo uye kurongeka kwekusimudzira kunovimbiswa, uye dambudziko rekusvibiswa kwePCR chigadzirwa chinogona kuitika panguva yakakura-yakakura genetic test inodzivirirwa.
Zvinotsanangurwa
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Kit zvikamu
Chikamu I | Buffer P1 |
Buffer P2 | |
6×DNA Loading Buffer | |
Chikamu II | 2× Leaf PCR NyoreTMMusanganiswa(UNG) |
Zvimiro&zvakanakira
■ Hapana kunotora nguva uye kunodhura kuchenesa DNA
■ Zvinyorwa zvishoma
■ Nyore-sample inogona kuiswa pasi pePCR reaction kana lysis reaction pasina kucheka kana kugaya
■ 2 × Sanganisa, kuderedza sampuli kurodha zvikanganiso uye reaction system yekugadzirira nguva
■ Kugadzirira kweFast-template kunogona kupedzwa mumaminitsi gumi, kuita kwePCR kunogona kupera mukati memaminitsi makumi mashanu.
■ High throughput-lysis reaction inogona kupedzwa mu96-tsime PCR ndiro
Kit parameters
Chishandiso: genetically modified identification, genotyping, nezvimwe.
Sample: chirimwa shizha
Chiyero: dhayamita 2-3mm (yakananga nzira), dhayamita 5-7mm (lysis nzira)
Kuonekwa kwemhando: chidimbu chechinangwa ≤1kb
Kuyerera kwebasa
Electropherogram
Vhiki mbiri dzePCR Mix yekuona mhedzisiro pasi pemamiriro ePCR amplification chigadzirwa kusvibiswa.