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Lnc-RT Heroᵀᴹ I (Ine gDNase) (Super Premix yekutanga-tambo cDNA synthesis kubva kuLncRNA)
Lnc-RT GambaTM Ini (Ine gDNase) iri rerekodhi transcript system yakanyatsogadzirirwa lncRNA kuti ikurumidze kubvisa genomic DNA kusvibiswa. 5 × gDNase Sanganisa inogona kukurumidza kubvisa yakasara genome muRNA pa42 ° C kwemaminetsi maviri, ichinyatso kudzivirira kukanganisa kwejenome pamigumisiro yeQPCR
5 × L-RT GambaTM Sanganisa ine Foregene LncRNA Reverse Transcriptase yakanyatsogadziriswa na Foregene, inova mhando nyowani yekudzosera transcriptase yakanangana ne lncRNA uye mamwe marefu maRNA matemplate akaomarara, ane simba rakasimba reRNA kubatana uye kwakanyanya kudzoreredza kurekodha kugona. Iyo yakagadziriswa sisitimu inoita iyo yekumashure transcript chiyero nekukurumidza uye inogona nyore nyore kunyora maRNA matemplate ane yakakwira GC zvemukati uye yakaoma yechipiri chimiro. Yekutanga tambo cDNA synthesis inogona kupedzwa pa 42 ° C mumaminitsi gumi nemashanu.
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Nguva Yechokwadi PCR Easyᵀᴹ-Taqman
Iyo 2X Chaiyo PCR NyoreTM Sanganisa-Taqman yakapihwa neiyo Chaiyo Nguva PCR NyoreTM-Taqman kit iyi nyowani premix system iyo inoshandisa chaiyo fluorescent probes yeReal Time PCR yekukwirisa maitiro, ayo anogona kuvandudza zvakanyanya kugadzirwa kwechigadzirwa uye kuita kwekunzwa. ROX inopihwa senge yekudzora dhayi mukati.
2X Real PCR NyoreTM Sanganisa-Taqman ine Foregene yakasarudzika inopisa-kutanga Taq DNA Polymerase. Inofananidzwa neyakajairika Taq enzyme, ine zvayakanakira yepamusorosoro kukwidziridzwa kugona, yakasimba yakananga yekuwedzera kugona uye yakaderera mismatch chiyero Inogona kudzora isiri-yakatarwa kukwidziridzwa uye nekuvandudza kunyatso kwePCR.
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Nguva Yechokwadi PCR Easyᵀᴹ-SYBR Green I kit
Iyo 2X Chaiyo PCR NyoreTM Sanganisa-SYBR yakapihwa neiyo Chaiyo Nguva PCR NyoreTM-SYBR Green I kit ndeye nyowani premix system iyo inoshandisa SYBR Green Ini yeNguva Yechokwadi PCR yekukwirisa maitiro, ayo anogona kwazvo kuvandudza chigadzirwa chaicho uye kugadzirisa kwekunzwa. ROX inopihwa senge yekudzora dhayi mukati. Iyo fluorescence kusimba kweichi kit is 3-5 nguva iyo yezvigadzirwa zvakafanana, izvo zvinogona kuve zvinonzwisisika uye intuitively kuratidza iko kukosheswa kwetatu template template.
2X Real PCR NyoreTM Sanganisa-SYBR ine Foregene yakasarudzika inopisa-kutanga Taq DNA Polymerase. Inofananidzwa neyakajairika Taq enzyme, ine zvayakanakira yepamusorosoro kukwidziridzwa kugona, yakasimba yakananga yekuwedzera kugona uye yakaderera mismatch chiyero Inogona kudzora isiri-yakatarwa kukwidziridzwa uye nekuvandudza kunyatso kwePCR.
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PCR Heroᵀᴹ (Iine Dhayi)
Iyo 2X Chaiyo PCR NyoreTM Sanganisa-SYBR yakapihwa neiyo Chaiyo Nguva PCR NyoreTM-SYBR Green I kit ndeye nyowani premix system iyo inoshandisa SYBR Green Ini yeNguva Yechokwadi PCR yekukwirisa maitiro, ayo anogona kwazvo kuvandudza chigadzirwa chaicho uye kugadzirisa kwekunzwa. ROX inopihwa senge yekudzora dhayi mukati. Iyo fluorescence kusimba kweichi kit is 3-5 nguva iyo yezvigadzirwa zvakafanana, izvo zvinogona kuve zvinonzwisisika uye intuitively kuratidza iko kukosheswa kwetatu template template.
2X Real PCR NyoreTM Sanganisa-SYBR ine Foregene yakasarudzika inopisa-kutanga Taq DNA Polymerase. Inofananidzwa neyakajairika Taq enzyme, ine zvayakanakira yepamusorosoro kukwidziridzwa kugona, yakasimba yakananga yekuwedzera kugona uye yakaderera mismatch chiyero Inogona kudzora isiri-yakatarwa kukwidziridzwa uye nekuvandudza kunyatso kwePCR.
