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Primer dhizaini-yekuona chaiyo, kugadzikana uye amplification kunyatsoita
Kunyatsozivikanwa kwekuona Muzviitiko zvakawanda, chinangwa chekugadzira primer ndechekuwedzera kutaridzika kwePCR.Izvi zvinotemerwa nekuwedzera kana kushoma kutarisika pesvedzero yezvakasiyana zvakawanda.Imwe shanduko yakakosha ndeyekutevedzana ku3′ kwekupedzisira kwekutanga.Zvakakosha, PCR assays yakagadzirirwa ...Verenga zvimwe -
Nucleic acid mishonga yapinda muzera regoridhe, ndeapi matekinoroji akakosha anoda kugadziriswa nekukurumidza?
Kwakabva: Medical Micro Mushure mekubuda kweCCIDID-19, majekiseni maviri eMRNA akakurumidza kubvumidzwa kushambadzira, izvo zvave kukwezva kutarisisa kwekuvandudzwa kwemishonga nucleic acid.Mumakore achangopfuura, akati wandei nucleic acid mishonga ine mukana wekuve blockbuster mishonga yakaburitsa ...Verenga zvimwe -
Ongororo yeKusimbisa Indexes yePrimer Probes muPreliminary Period yePCR Reagents.
Kuona mashandiro emaprimers uye probes muchikamu chekutanga chePCR reagents uye kuona akanyanya akakodzera maitiro ekuita ndizvo zvinodikanwa kuti uve nechokwadi chekufambira mberi kwakanaka kwekuyedzwa kwepamutemo.Saka isu tinoda sei kusimbisa iyo primer probe mukutanga ...Verenga zvimwe -
Sampling ruzivo mapoinzi ayo vaongorori vanofanira kuona
Kuyedzwa kwerabhoritari kunotanga nekuunganidzwa kwemuenzaniso, uye kuunganidzwa kwemuenzaniso ndiko kuri nyore kusatarisisa.Chinhu chinonyanya kukosha pakuunganidzwa kwemuenzaniso kusarudza mhando yemuenzaniso, shandisa maturusi esampling akakodzera, uye kuita zvekufambisa zvine musoro uye kugadzirisa.I.Sample type Common sam...Verenga zvimwe -
Iyo yekupedzisira mhinduro kune nucleic acid aerosol kusvibiswa
PCR nzira uye nucleic acid aerosol kusvibiswa mune nucleic acid yekuongorora marabhoritari yakafanana nemativi maviri emari.Tinogona chete kusarudza kuva nazvo kana kuti kwete, asi hatigoni kusarudza kuti tinochida here kana kuti tochishandisa.1. Kuongororwa kweDNA remover Kuti uwane kubviswa kwenzvimbo ...Verenga zvimwe -
Kukurumidza kuzivikanwa kwezvinomera zvinoshanduka
Sechitsva murabhoritari, harisi basa rakanaka kuongorora zvirimwa zvakanaka kubva muboka rezvirimwa zvine mwero wakaderera wekutendeuka.Chekutanga, DNA inofanirwa kutorwa kubva kunhamba huru yemasampuli imwe neimwe, uyezve majini ekunze anozoonekwa nePCR.Nekudaro, mhedzisiro inowanzove isina chinhu ...Verenga zvimwe -
Tsanangudzo yakazara yeakakosha mapoinzi eSARS-CoV-2 nucleic acid bvunzo, nei paine manyepo asina kunaka uye bvunzo dzezve?
Muchikamu chekutanga chekubuda, nekuda kwekukurumidza kukura, kukurumidza kuongororwa kwevanofungidzirwa varwere ndiyo kiyi yekudzivirira COVID-19.Mamwe akatenderwa nucleic acid yekuona reagents ane nguva pfupi yekuvandudza, uye kune matambudziko akadai sekumhanyisa kuita kusimbiswa, kusakwana ...Verenga zvimwe -
Chii chinonzi SNP?Topics on Population Genetics
Iwo mavara matatu SNP ari kwese kwese mukudzidza kwehuwandu hwemajini.Pasinei nekutsvaga kwechirwere chevanhu, chimiro chezvirimwa, kushanduka kwemhuka uye mamorekuru ecology, SNPs inodiwa sehwaro.Nekudaro, kana iwe usina nzwisiso yakadzama yemazuva ano genetic ...Verenga zvimwe -
Nzira yekuvandudza sei kushanda kweLncRNA reverse transcription?
lncRNA features: 1. lncRNAs inowanzoreba, ine simba rekutaura uye nzira dzakasiyana-siyana dzekubatanidza panguva yekusiyanisa;2. Kuenzaniswa nekodhi yemajini, lncRNA kazhinji yakaderera;3. Mazhinji lncRNA ane pachena yenguva uye nzvimbo yekutaura chaiyo mukuita ...Verenga zvimwe -
Mana Main Solutions YePCR Chigadzirwa Kusvibisa Kudzora
1: Tsiva zvinhu zvekuyedza munguva Seta (NTC) kutonga kwakashata uye dzokorora kakawanda.Kana zvangoonekwa kuti mune PCR chigadzirwa kusvibiswa murabhoritari, tsiva zvese zvekuyedza nenguva.Zvakadai se: dilute zvakare uye gadzirira maprimers, re-sterilize iyo pipette tip, E ...Verenga zvimwe -
Maviri maviri-anoshanda RT-PCR ma enzymes
Traditional reverse transcriptases haigoni kushivirira tembiricha yepamusoro (tembiricha yakakwana yeMMLV chiitiko i37-50°C, uye AMV i42-60°C).Iyo yakanyanya kuomesesa hutachiona RNA haigone kudzoreredza zvinobudirira kunyorwa mu cDNA pakadzika tembiricha, zvichikonzera kugona kwekuona Kuderedza.Tra...Verenga zvimwe -
Nucleic acid isothermal amplification tekinoroji
PCR ndiyo inonyanya kushandiswa nucleic acid amplification tekinoroji uye inoshandiswa zvakanyanya nekuda kwekunzwa kwayo uye kujeka.Nekudaro, PCR inoda kudzokorora kupisa denaturation uye haigone kubvisa zvipingamupinyi zvekuvimba nezviridzwa nemidziyo, iyo inomisa kushanda kwayo mukiriniki ...Verenga zvimwe
