Kune marudzi maviri makuru enjodzi mumarabhoritari ePCR: njodzi dzebiosafety uye njodzi yekusvibisa nucleic acid.Iyo yekare inokuvadza vanhu uye nharaunda, uye iyo yekupedzisira inokanganisa mhedzisiro yePCR bvunzo.Ichi chinyorwa chiri pamusoro pePCR labhoritari yekutarisa njodzi nzvimbo uye inoenderana nenjodzi nhanho dzakaunzwa kwauri.

01 Chikamu chePCR laboratori

1. Murabhoritari yekuongorora zvipenyu

Zvinoenderana nezvinodiwa zveChinyorwa 1.1 cheBasic Setting Standards yeClinical Gene Amplification Testing Laboratories, PCR marabhoritari anowanzo kuve nenzvimbo ina: reagent yekuchengetera uye nzvimbo yekugadzirira, nzvimbo yekugadzirira, nzvimbo yekusimudzira, uye nzvimbo yekuongorora chigadzirwa.Kana iyo chaiyo-nguva fluorescent PCR nzira yakashandiswa, nzvimbo yekusimudzira uye nzvimbo yekuongorora inogona kusanganiswa mune imwe nzvimbo;kana iyo yakazara automated PCR analyzer yakashandiswa, iyo specimen yekugadzirira nzvimbo, nzvimbo yekusimudzira uye nzvimbo yekuongorora inogona kubatanidzwa munzvimbo imwe.

"Bhuku rekushanda reNew Coronavirus Nucleic Acid Testing muMedical Institutions (Trial Version 2)" rinotara kuti musimboti, marabhoritari ari kuita ongororo itsva yeconavirus nucleic acid anofanirwa kumisikidza nzvimbo dzinotevera: reagent yekuchengetedza uye nzvimbo yekugadzirira, nzvimbo yekugadzirira, kukwidziridza uye nzvimbo yekuongorora chigadzirwa.Idzi nzvimbo nhatu dzinofanira kunge dzakazvimiririra zvakakwana kune imwe neimwe munzvimbo yemuviri, uye hapangave nekutaurirana kwakananga nemhepo.

2. Imba yekugadzirira yemuenzaniso

Kunyangwe samples inogona kugadzirirwa nyore munzvimbo yekugadzirira sampuli, yakakosha kamuri yekugadzirira ichiri kudikanwa kana uchibata neakaomarara masampuli uye nhamba huru yemasampuli.Imba yekugadzirira yekuenzanisira ine njodzi yakakura yekuchengetedzwa kwebiological uye nucleic acid kusvibiswa.

3. Imba yekurapa tsvina

Kuraswa kwemarara kwakashata kuchaunzawo njodzi huru dzebiosafety uye nucleic acid kusvibiswa murabhoritari.Naizvozvo, imba yekurapisa tsvina inoda kugara ichitariswa.

02 Njodzi yekutarisa nzvimbo muPCR marabhoritari

Akapatsanurwa marabhoritari akakamurwa kuita sampuli yekugadzirira kamuri, reagent yekuchengetera uye nzvimbo yekugadzirira, sampuli yekugadzirira nzvimbo, amplification uye chigadzirwa chekuongorora nzvimbo, uye marara yekurapisa imba.

Zvinoenderana nerudzi rwesampling saiti, yakakamurwa kuita pamusoro, chiridzwa, sampuli, yekutarisa kwezvakatipoteredza uye pipette.

Njodzi yengozi inotangira kubva pasi kusvika kumusoro kubva nyeredzi imwe kusvika kune nhatu nyeredzi ★★★.

1. Imba yekugadzirira yemuenzaniso:

Inoshandiswa kunyoresa, kugadzirira uye kusashanda kwemasamples, uye njodzi yekuchengetedza biological ndiyo yakanyanya.Nekuti sampuli hadzina kubviswa uye kukwidziridzwa, kunze kwemapipette anowanzo sangana nemasampuli, njodzi yekusvibiswa kwenyucleic acid mune zvimwe zvikamu yakaderera.

1-4 Sampling panzvimbo dzekutarisa

5-8 Sampling panzvimbo yekutarisa

9-12 yekutarisa nzvimbo sampling

1. Reagent yekuchengetedza uye nzvimbo yekugadzirira:

Inoshandiswa kugadzirira kwekuchengetedza reagents, kugovera reagents uye kugadzirira kweamplification reaction musanganiswa, pamwe nekuchengetedza uye kugadzirira kwezvinhu zvinoshandiswa zvakadai se centrifuge tubes uye pipette mazano.Iko hakuna kutarisana zvakananga nemasampuli uye hapana yakanaka nucleic acid munzvimbo ino, saka njodzi yebiosafety uye nucleic acid yekusvibiswa njodzi yakaderera.

13-16 Sampling panzvimbo dzekutarisa

17-22 Sampling pakuongorora
3. Muenzaniso wekugadzirira nzvimbo dzenzvimbo

Inoshandiswa kuvhura dhiramu rekutamisa, inactivate specimen (kana zvichibvira), kubvisa nucleic acid uye kuwedzera kune amplification reaction tube, etc. Iyi nzvimbo inogona kusanganisira kugadzirisa uye kuvhurwa kwemasampuli, ngozi yekuchengetedzwa kwehupenyu yakakwirira, uye nucleic acid extraction inoitwa, uye njodzi yekusvibiswa kwenyucleic acid iri pakati nepamusoro.

29 Sampling panzvimbo dzekutarisa

4. Amplification uye chigadzirwa chekuongorora nzvimbo:

Inoshandiswa kune nucleic acid amplification.Iyi nzvimbo haisanganisire sampuli kugadzirisa, uye njodzi yebhayoloji yekuchengetedza yakaderera.Iyo nucleic acid amplification inonyanya munharaunda iyi, uye njodzi yekusvibiswa kwenyucleic acid ndiyo yakanyanya.

38 Sampling panzvimbo dzekutarisa
5. Imba yekurasira tsvina:

Inoshandiswa kune yakakwira pressure processing yemasampuli.Njodzi dzekuchengetedzwa kwebhayoloji dzinosanganisirwa mukugadziriswa kwemasampuli munzvimbo ino dzakanyanya.Iyo nucleic acid amplification zvigadzirwa zvinokurudzirwa kubatwa semarara ekurapa.Kudzvinyirirwa kukuru hakukurudzirwi, uye njodzi yekusvibiswa kwenyucleic acid yakaderera.

43-44 Sampling panzvimbo dzekutarisa

03 Shandisa

Panguva ino takanyora 44 mapoinzi ekutarisa.Zvinofungidzirwa kuti vanhu vazhinji vanofanira kubvunza, vanofanira kuita mapoinzi akawanda kudaro here?Hongu, ita zvose!Ini ndinokurudzira kuti utange waongorora njodzi yerabhoritari yako, iyo inogona kuitwa zvinoenderana nenjodzi kubva kumusoro kusvika yakaderera, unogona zvakare kutarisa mhando imwe chete yemasampuli pamwe chete, kana iwe unogona kugadzira sampling chirongwa chekutarisa nguva dzose.Muchidimbu, laboratori yega yega inogona kuita hurongwa hwayo hwekuita zvinoenderana nemamiriro ayo.Njodzi huru yekuyedza marabhoritari ndeyekufuratira njodzi.