Mhoro, shamwari!
Mushure mekuita nucleic acid kudhirowa kwemakore akawanda kudaro, munhu wese anonyatsoziva chokwadi chekuti kunaka kweiyo nucleic acid kutorwa ndiyo kiyi yekubudirira uye nekururama kwezviedzo zvinotevera.
Nguva zhinji, isu tinongoitira hanya kana reshiyo yeOD260/OD280 iri mukati meiyo yakakwana renji.Sezvizvi...
Sezvo chirevo chichienda, haugone kutaura zvakanyanya, uye haugone kufara kare kare.
Tanga wateerera Mupepeti akuudze kanyaya!
Ngano dzakanaka kudaro dzinowanzoitika mumarabhoritari anoita nucleic acid kutorwa.
Waifunga kuti wangotora RNA chete, Sorry!
Kutaura zvazviri, kungoti hauzivi kuti Mr Protein vari kuvanda chinyararire.
Unofunga kuti unoda here kuita kuti Mr Protein vaoneke neOD260/OD280 imwe chete?
Mudiki zvikuru!Zviri nyore!
Ngatishandisei yakanyanya kusimba sirivheri nzira yekukubatsira iwe kuona mapuroteni akavanzwa muRNA!
| Chigadzirwa chakashandiswa | OD260/280 | Mchiridzwa chekuyeresa |
| Foregene | 2.09 | Thermo ScientificTMNanoDropTMPoshi Ultra-trace UV-Vis Spectrophotometer |
| Trizol | 1.91 |
Kare, kare kare, mapuroteni neRNA vaiva shamwari dzepedyo.
Mr Protein vanonyara zvakanyanya.
Saka yagara iri iyo inoshanda uye inofara RNA yekutamba nayo.
Nerimwe zuva.
RNA yakaudza Mr Protein kuti yaizotorwa nevamwe, uye Mr Protein vakasuwa zvikuru.
RNA zvakare inozeza kuna Mr protein, ndokuti: "Ko ini ndikakuverevedza, unohwanda muhembe dzangu, edza kuti usaonekwa nevamwe, zvakanaka?"
VaProtein vakabva vatanga kunyemwerera ndokubvuma nomufaro.
Pazuva rekugamuchirwa, Mr Protein vakanyatsovanda muhembe dzeRNA uye havana kuwanikwa.Akatevera munhu wokurera kumusha ndokurarama upenyu hunofadza kubvira ipapo.
Kuenzanisa kweRNA kutorwa neForegeneSeroTotalRNA Extraction Kit
uye T Kambani's Trizol Reagent
Isu tinokwanisa kuona zvakajeka bhenji pamukoto 4 (Trizol), nepo nzira 3 (Forgene) yakachena.Mhedzisiro yacho yakasimbisa kuti mapuroteni eRNA akasviba akaburitswa nenzira yeTrizol aive achiri madonhwe mazhinji!Uye nzira yechitatu (Forgene) inenge isina kusvibiswa kweprotein nekuti muongorori akashandisa iyo Foregene RNA yekubvisa kit!(Kushambadza kwakauya ndisingatarisire)
Mamwe mashoko:
https://www.foreivd.com/cell-total-rna-isolation-kit-product/
Nguva yekutumira: Feb-10-2022
