PCR nzira uye nucleic acid aerosol kusvibiswa mune nucleic acid yekuongorora marabhoritari yakafanana nemativi maviri emari.Tinogona chete kusarudza kuva nazvo kana kuti kwete, asi hatigoni kusarudza kuti tinochida here kana kuti tochishandisa.

1. Kuongororwa kweDNA remover

Kuti uwane kubviswa kwenzvimbo kweiyo nucleic acid aerosol kusvibiswa, zvinofanirwa kutanga kuongorora kunze maDNA anobvisa anogona kubvisa nucleic acids munzvimbo yemvura.Nekuti hakuna akawanda maDNA anobvisa anoshanda chaizvo.Kune iyo nzira yekuyedza, ndapota tarisa kune: DNA inobvisa haigone kuve "yakavanzika kona" yerabhoritari!

Muchiyedzo ichi, zana makopi/μL (CT angangoita makumi matatu nematanhatu) eASFV plasmid uye DNA inobvisa yakaverengerwa nedigital PCR yakasanganiswa muhuwandu hwakaenzana, uye ndokuita pakamuri tembiricha yegumi min, 20min uye 30min zvakateerana.Mushure mekutora nucleic acid, qPCR amplification yakaitwa.Kudzora kwakanaka kwakasanganiswa neplasmid nemvura kwakaenzaniswa.Sezvo kuedza kusina kupedzwa panguva imwe chete, panogona kunge kune kumwe kutsauka pakati pemigumisiro, asi haikanganisi mhedziso yekuedza.Kusvika pari zvino, ndaongorora gumi rekutengesa DNA rinobvisa zvigadzirwa.Chete Nhamba 1, Nhamba 6 uye Nha.Zvimwe zvinogadzirwa hazvina zvazvinoita.

Tafura 1 Nucleic acid kubvisa mhedzisiro yeinotengeswa DNA inobvisa

2. Kuedza kweDNA kubvisa mhedzisiro yemishonga inouraya utachiona inowanzo shandiswa

1. Kuongororwa kwemishonga inouraya utachiona inowanzo shandiswa

Kune marudzi akawanda emishonga inouraya utachiona inowanzoshandiswa mumarabhoritari: aldehydes, phenols, alcohols, quaternary ammonium salts, peroxides, chlorine preparations, uye acids nemabhesi.Mhedzisiro yekuuraya utachiona yeaya majekiseni pahutachiona yakasimbiswa zvizere nekuyedza, asi Hapana akakwana data rekuyedza pamusoro pekuora kweiyo nucleic acids.Nucleic acid yekuongorora marabhoritari inoda disinfectant iyo isingangove neyakanaka disinfection mhedzisiro pane tupukanana, asi zvakare inogona kukanganisa DNA.Mhedzisiro yebvunzo inoratidza kuti maviri chete chlorine gadziriro ye84 disinfectant uye trichloroisocyanuric acid uye 1 M hydrochloric acid inosangana nezvinodiwa.Sezvo 1 M hydrochloric acid ichikuvadza zvakanyanya, zvinokurudzirwa kuti kugadzirira kweklorini kushandiswe sekurasa marara mumarabhoritari ekuongorora nucleic acid.Nekudaro, kugadzirira kweklorini kunokanganisa simbi uye haigone kushandiswa kuuraya hutachiona hwezviridzwa nemidziyo.

Tafura 2 DNA kubvisa mhedzisiro yemhando dzakasiyana dzemishonga inouraya utachiona

2. Iko kuderera kunobudirira kwekugadzirisa kweklorini yekugadzirira

Chlorine-based disinfectants ine simba rakasimba reDNA degradation, asi nekuda kwekuora kwesimbi uye kushatirwa, inogona kushandiswa kuchenesa pasi, pasiri-simbi countertops, matipi ekunyorovesa, centrifuge machubhu uye zvimwe zvinhu zvekuedza.

Maererano ne "New Coronary Pneumonia Epidemic Disinfection Technical Guidelines": Zvinosvibisa (ropa revarwere, secretions uye marutsi) inowanikwa chlorine 5g/L-10g/L chlorine-ine disinfectant;pasi, madziro, uye zvinhu zvinogara zvichishandisa chlorine inowanikwa 1g/L Chlorine mushonga wekuuraya utachiona: mbatya, mibhedha nemimwe micheka zvinotanga zvakanyikwa mumishonga inouraya utachiona hwechlorine ine 0.5g/L inowanikwa chlorine kwemaminitsi makumi matatu, zvobva zvacheneswa semazuva ose.

Tafura 3 DNA kubvisa maitiro ezvakasiyana-siyana zve chlorine disinfectant

Mhedzisiro yebvunzo inoratidza kuti: kana chlorine ikashanda yakakura kupfuura kana kuenzana ne1.2 g/L, mushonga une chlorine-ine utachiona unokwanisa kukanganisa zvachose makopi zana/μL plasmid kana ichishandiswa kwemaminitsi mashanu.Paunenge uchibata kwemaminetsi gumi, mishonga yekuuraya utachiona makumi masere nemana ine chlorine inoshanda yakakura kupfuura kana kuenzana ne0.6 g/L inogona kukanganisa zvachose makopi zana/μL eplasmid.

3. Kuedza kubvisa mhepo kwakasvibiswa ne nucleic acid aerosol

Nzira yekuchenesa mhepo yakasvibiswa ne nucleic acid aerosols?Mifungo mizhinji ndeyekugadzira nharaunda yakasvibiswa neaerosol wobva waichenesa, kana kuchenesa nucleic acids mushure mekusvibiswa.Kwandiri, kuedza kusingagone kudzokororwa uye mhedzisiro yekuyedza haigone kuongororwa zvakanyanya haina zvazvinoreva, saka ndakakwereta dzimwe nzira dzekuuraya hutachiona hwemhepo.Ona "GB27948-2020 General Zvinodiwa zveMhepo Disinfectants" uye "Disinfection technical Specifications"

1. Test zvinhu

1.1 DNA remover: Muchiedzo ichi, maviri anotengeswa DNA anobvisa 6 uye 8 muTafura 1 akasarudzwa.

1.2 Test plasmid: Tichifunga kuti kukosha kweCT yekusvibiswa kwakawanda murabhoritari kuchakwira kupfuura makumi matatu, huwandu hweASFV gene plasmid inoshandiswa panguva ino ingangoita zana makopi/μL, uye fluorescent quantitative PCR CT ingangoita 31.07.Wedzera plasmid kune inoyerera donje swab uye mhepo yakaoma.

1.3 Chiridzwa chekuyedza: Bilingkehan KVBOX chiridzwa chekuuraya utachiona, icho chinotsigirwa nemutsa neShenzhen Runlian Environmental Technology Co., Ltd., ine mhepo yakanaka yekuuraya utachiona nehydrogen peroxide disinfectant, uye inogona zvinobudirira kuuraya spores nezvimwe tupukanana.

1.4 Nzvimbo yekuyedza: hwindo rekutamisa rakavharwa rinosvika 0.1 cubic metres.

2. Nzira yekuedza

Huwandu hwekuuraya hutachiona hwakaiswa pamushini wekuuraya hutachiona ndeye 10ml, uye iyo yekumisikidza iri 100ml/m3, iyo yakakwira zvakanyanya kupfuura "GB27948-2020 General Zvinodiwa zveMhepo Disinfectants": kana uchishandisa aerosol spray disinfection, huwandu hwekuuraya hutachiona hunofanirwa kunge huri ≤10 ml/m3.