• facebook
  • linkedin
  • youtube

Kuyedzwa kwerabhoritari kunotanga nekuunganidzwa kwemuenzaniso, uye kuunganidzwa kwemuenzaniso ndiko kuri nyore kusatarisisa.Chinhu chinonyanya kukosha pakuunganidzwa kwemuenzaniso kusarudza mhando yemuenzaniso, shandisa maturusi esampling akakodzera, uye kuita zvekufambisa zvine musoro uye kugadzirisa.

I.Sample type

Mhando dzemienzaniso dzakajairika ndedzinotevera:

dsfgsd

Iyo sampling nzira inogona kureva tekinoroji yekutarisisa nharaunda yekoronavirus nyowani mumusika wekutengesa zvekurima (bazaar)":

1. Swab samples kubva pahuro swabs, maoko, zvipfeko uye zvimwe zvinhu zvevashandi: zvinotarisirwa kushandisa hutachiona hwekuchengetedza hutachiona muhutachiwana sampling chubhu uye kunyatsopinza sampling cotton swabs.Pamusoro pevhu) inodzokororwa kushandiswa uye kusukwa kwekanopfuura katatu.Panguva imwecheteyo, zvinodikanwa kuita akawanda-point akagoverwa sampling pamusoro pechinhu chakatorwa.

2. Food surface swab samples: Zvikafu zvekudya hazvigone kuunganidzwa zvakananga.Chikafu chinotorwa chinofanira kuparadzaniswa nemazvo chochengetwa mubhegi rakachena rekutora sampling tisati tanhonga masampu.Zvinotarisirwa kushandisa hutachiona hwekuchengetedza mhinduro muhutachiona sampling chubhu, mushure mekunyorovesa zvizere sampling donje swab, kudzokorodza smear uye sukurudza pamusoro pechikafu sampuli inounganidzwa kanopfuura katatu.Panguva imwecheteyo, zvinodikanwa kuita akawanda-poinzi akagoverwa sampling pane sampuli yepamusoro.

3. Sewage samples: Zvinoenderana nekugoverwa kweiyo drainage system mumusika, sarudza 2-3 sewage sampling nzvimbo, uchitarisa mukati mepombi network yekuunganidza, kuzasi kweiyo nzira yekuyerera kwemvura, kana kubatana netaundi yepombi pombi.Kuti utore sampuli ye swab, inyudze mumvura yetsvina nesampling donje swab kuti iite adsorb tsvina uye woisuka muchubhu yesampling kanopfuura katatu.Kuti utore sampuli dzetsvina, shandisa polyethylene mabhodhoro epurasitiki kuunganidza 30-500 mL yemvura yetsvina samples;yekuunganidza tsvina ine vhoriyamu inopfuura 500 mL, polyethylene mabhakiti epurasitiki kana pane-saiti mvura sampu yakakosha yekupfumisa michina inogona kushandiswa.Panguva imwecheteyo, zvinodikanwa kuitisa akawanda-point akagoverwa sampling kune sewage sampling nzvimbo.

4. Mienzaniso yemhuka: Kumhuka mhenyu, sampling swabs inogona kushandiswa kuunganidza muviri pamusoro swabs, oropharyngeal swabs uye anal swabs, uye tsvina kana secretion samples inogonawo kuunganidzwa uye kurekodhwa zvinoenderana papepa rekodhi.Kune sampuli dzemhuka dzakaitwa ganda, nezvimwewo, shandisa machira edonje kuunganidza miviri yavo uye masample emuviri wemhango.Zvinotarisirwa kushandisa mushonga wekuchengetedza hutachiona muhutachiwana sampling chubhu kuti upinde zvizere mumuenzaniso wedonje swab, uye wozodzokorora kushandiswa kwepamusoro pechikafu chekudya chinotorwa.Shabu kanopfuura katatu.Panguva imwecheteyo, zvinodikanwa kuita akawanda-point akagoverwa sampling pane sampuli yepamusoro.

5. Mimwe midziyo: midziyo yekutakurisa nekuberekesa mhuka yakaita semakeji kana matangi ehove.Chekutanga, tarisa kana kunzwisisa mhando dzemhuka dzakachengetwa nekuberekeswa mugaba, uye tora masampuli eswab pamadziro emukati memudziyo kana masamples emvura ezviri mukati.

6.Kuunganidza sampuli dzeaerosol munzvimbo dzinoungana vanhu, nzvimbo dzekutengesera dzisina mhepo zvakanaka, mahofisi, uye dzimba dzekuzororera.

II.Sampling zvishandiso
1. Ropa rekuunganidza chubhu
Kana iwe uchida kuita nucleic acid kuongororwa uye anticoagulant samples gare gare, zvinokurudzirwa kushandisa vacuum ropa mudziyo une EDTA anticoagulant kuunganidza ropa;kune serum samples, zvinokurudzirwa kushandisa vacuum ropa mudziyo pasina anticoagulant.

fdsgfd

2. Sampling swab

Inokurudzirwa kushandisa polypropylene swabs panzvimbo ye calcium alginate swabs kana swabs nematanda emapuranga, nokuti inogona kunge ine zvinhu zvinomisa mamwe mavhairasi uye inhibit PCR kuongororwa.Swabs ane misoro yekotoni haigone kushandiswa, nekuti machira ekotoni ane adsorption yakasimba yeprotein uye haabudiswe nyore mune inotevera kuchengetedza mhinduro.

Mu "Technical Details for 10-in-1 Mixed Collection uye Detection of New Coronavirus Nucleic Acids", zvinonyatso taurwa kuti "sampling swab inofanira kugadzirwa nepolyester, nylon nezvimwe zvisiri zvedonje uye zvisiri-calcium alginate, uye mubato unofanirwa kugadzirwa nezvinhu zvisiri zvehuni.Nzvimbo yekupwanya inenge 3cm kubva pamusoro pemusoro we swab, iri nyore kutyora. "

CDC yekuUnited States yakataurawo kuti “Shandisa chete mafiber swabs ane epurasitiki kana waya.Usashandise calcium alginate swabs kana swabs nemapuranga emapuranga,sezvo angave aine zvinhu zvinomisa mamwe mavhairasi uye anotadzisa PCR kuyedzwa.

Parizvino rinoshandiswa zvakanyanya chirasa chinoraswa naironi inoyerera yakasanganiswa netsvimbo yepurasitiki.Iyo swab inoyerera ine zvirinani kunyura kwemvura uye kuburitsa mwero.Zvinoenderana nenzvimbo dzakasiyana dzesampling, iyo swab musoro zvakare ine hukuru hwakasiyana uye fiber.Ukobvu.”

fsdg

3. Sample yekufambisa kuchengetedza mhinduro

Virus inactivation kuchengetedza mhinduro: huwandu hwakakodzera hwe guanidine salt concentration uye zvakasiyana-siyana zvehutachiona lysis components zvinogona kushandura hutachiona zvakananga kuburitsa nucleic acid, inactivate RNase uye DNase, uye ive nechokwadi chekuvimbika kweiyo nucleic acid, inogona kushandiswa kunotevera qPCR kuonekwa.Haikwanise kushandiswa pakurima hutachiona uye kuzviparadzanisa nevamwe.

Kutakura kwehutachiona uye kuchengetedza mhinduro: ine munyu, amino acids, mavhitaminzi, glucose uye mapuroteni anodiwa kuti hutachiona hurarame, hunogona kuchengetedza basa rehutachiona.Inogona kushandiswa kuita nucleic acid kutorwa uye kuona hutachiona mune ramangwana, uye inogona kushandiswawo kurima nekuparadzanisa hutachiona.

4. Sample kuchengetedza chubhu uye sampuli bhegi

Mucherechedzo wechubhu yekuchengetera inofanira kunge iri chubhu yepurasitiki isina mbereko ine screw cap isingaite adsorb nucleic acid.Imwe neimwe chubhu yemuyedzo inofanirwa kuvharwa nebhegi rekuenzanisira kudzivirira kubuda uye kusvibiswa kwesample.

fdsdf

III.Zvinokanganisa zvinhu mumuenzaniso

Zvinokanganisa zvinhu muPCR kuyedza

1. endogenous kupindira

Zvinhu zviripo mumuenzaniso wemuviri zvinogona kukanganisa kuonekwa kwezvimwe zvinhu.

Ropa:heme (> 1mg/ml), hemin (> 0.1ng/μl), triglycerides, IgG, nezvimwewo;

Weti:Urea (20mM);

Chituru:chirimwa polysaccharides, cholate;

Tissue:proteinase, collagen;

Mukaka: proteinase, calcium ion;

Zvinodhaka: mishonga inorwisa mabhakitiriya, antiviral mishonga, mahomoni, nezvimwe.

zvimwe.

2. exogenous kupindira

Inobva kune zvinhu zvinogona kukanganisa kuonekwa kwezvimwe zvinhu mu vitro.Sample additives uye zvinhu zvinogona kubatwa panguva yekuunganidza sampuli uye kugadzirisa:

Test zvinhu: serum separation gel, sampuli yekuunganidza mudziyo uye rabha chivharo, catheter, catheter flushing fluid, girovhosi poda, nezvimwe.

Anticoagulant: heparin (> 0.1 U/mL), EDTA (> 0.5 mM), nezvimwewo;

Organic solvents: isopropanol (> 0.1 IU / ml), betaine, dimethyl sulfoxide, formamide, glycerin, PEG, nezvimwewo;

Madisinfectants: doro (ethanol> 1%), aldehydes, phenols (phenol> 0.2%), peroxides, yakasimba acids (HCL), alkali yakasimba (NaOH), nezvimwewo;

Detergent:SDS (> 0.005%);

Ivhu: Humus

Dyestuff: Indigo

zvimwe.

Pasi pemamiriro chaiwo ekunze ekuenzanisira, sekuunganidza masampuli ezvakatipoteredza, masampuli anogona kunge aine zvinhu zvakasiyana-siyana zvinokanganisa, uye kuumbwa kwezvinhu zvinokanganisa munzvimbo imwe neimwe kwakasiyana, izvo zvinoda kuti PCR kupindira kuyedza kuitwe zvinoenderana nemamiriro enzvimbo.

IV.Sample kuchengetedza uye zvekufambisa mamiriro

Mienzaniso yekiriniki ichangobva kuunganidzwa inofanira kutumirwa kurabhoritari mukati memaawa maviri kusvika mana mushure mekuunganidzwa pa2°C kusvika pa8°C.Chengetedza pa4℃ mukati ma24h.Mienzaniso inoshandiswa kuparadzanisa hutachiona uye nucleic acid yekuona inofanira kuongororwa nekukurumidza sezvinobvira.Mienzaniso inogona kuongororwa mukati maawa makumi maviri nemana inogona kuchengetwa pa 4 ° C;zvienzaniso zvisingakwanisi kuonekwa mukati memaawa makumi maviri nemana zvinofanira kuchengetwa pa -70 ° C kana pasi (kana zvisiri- Chengeta pa 70 ° C mufiriji pa -20 ° C).

Pfupiso:Iko kuunganidzwa kwakamisikidzwa, kutakurwa uye kuchengetwa kwemasamples kwakakosha kune kunotevera kuyedzwa.Iwo emanyepo emanyepo ebvunzo dzakawanda dzemurabhoritari anokonzerwa nekuunganidza zvisirizvo, kutakura uye kuchengetwa kwemasamples izvo zvinotungamira mukuderedzwa kwehutachiona nucleic acid.Nekudaro, marabhoritari mazhinji achiri kufuratira kukosha kwesampling.Ndinovimba chinyorwa ichi chinogona kumutsa kutarisisa kwemunhu wese kune sampling uye kuita mamwe esainzi uye ane musoro sampling!

Related product:

SARS-CoV-2 Variant Nucleic Acid Detection Kit (I) (Multiplex PCR Fluorescent Probe Method)-Yekuonekwa kweMutants Kubva kuBrazil, South Africa, uye UK


Nguva yekutumira: Sep-17-2021