• facebook
  • linkedin
  • youtube

Nhanganyaya:

Mamorekuru eRNA anogona kudyidzana zvakanyanya nemamwe mamorekuru ehupenyu, akadai seprotein, DNA neRNA, uye nekudaro aripo mumasero uye zvipenyu muchimiro che macromolecular complexes.Pakati pavo, RNA-protein complexes ndiko kuvapo uye kushanda kweRNA Imwe yemhando dzakakosha dze.RNA kudonhedza pasi inzira yakasarudzika yekuongorora uye kuona mamorekuru eRNA, kunyanya mapuroteni anodyidzana elncRNA mamorekuru, uye inocherechedzwa neruzhinji rwevaongorori uye nemvumo yepasirese mabhuku ezvidzidzo.Nhasi FORREGENE ichagovana newe iyo yekuyedza musimboti uye tekinoroji maitiro eRNA kudhonza-pasi, pamwe unogona kuishandisa.

1. Nheyo yekuyedza yeRNA inodonhedza tekinoroji:

In vitro transcription uye synthesis yebiotin-yakanyorwa chinangwa lncRNA kana lncRNA zvimedu (kusanganisira zvimedu zveRNA zvinoshandiswa sezvakanaka uye zvisina kunaka kudzora), uye incubate nemasero ezvinyorwa;shandisa avidin-anchored magnetic beads kuunganidza RNA-protein kana RNA-RNA complexes, Mushure mekunyatsogeza, yakaoma inotorwa.Kana uchinge waona puroteni yakanangana nemorekuru inodyidzana nelncRNA, ita protein electrophoresis paRNA-dhonzera pasi chigadzirwa, tarisa puroteni yakanangwa nekusvibiswa kwesirivheri, uye cheka gel ye mass spectrometry;zvakare ita zvakananga kumadokero blot verification pane yekudhonza-pasi chigadzirwa;kana yaonekwa Kune RNA yakananga mamorekuru anodyidzana nelncRNA, RNA-kudhonza pasi zvigadzirwa zvinoiswa pasi peRNA kudhirowa uye chaiyo qRT-PCR yekuona inoitwa.

2. RNA inokwevera pasi tekinoroji yekuyedza maitiro:

itsva

3. Tekinoroji sevhisi yeRNA kudonhedza pasi ye FOREGENE:

Sero tsika

Kugadzirira kwesero yakazara / nyukireya / plasma protein yakabudiswa

RNA inokwevera pasi kuedza

SDS-PEJI electrophoresis, tsvina yesirivha

Mass spectrometry uye WB miedzo

Experimental report

4. Kuedza nyaya

Iyi inotevera nhamba inoratidza mhedzisiro yeFJ Bio-Bio's RNA kudonhedza pasi uye SDS-PAGE electrophoresis yekuona, umo museve mutsvuku unomaka musiyano bhendi:

itsva2

Boka rekutsvagisa raProfessor Song Xu weSichuan University rakaunganidza makore gumi nemashanu ekuunganidza kwenguva refu mumunda welncRNA research.Foregene Bio naProfessor Song Xu boka rekutsvagisa rakabatana kuburitsa iyo RNA yekudonhedza tekinoroji yelncRNA, iyo inokupa mhedzisiro inogutsa pakuedza kwako.


Nguva yekutumira: Jun-11-2021