Nezve RNA kutorwa

Makore makumi matatu apfuura, kudhirowa kweRNA, izvo zvataigona kufunga yaive nzira yekuwana RNA yakakwana, zvisinei, pakanga pasina chinodiwa chekumhanyisa kumhanya (Yekutanga-chizvarwa Techology nzira yeTrizol yakazvarwa).Neiyo iterative yekuvandudza yeRNA yekubvisa tekinoroji, zvatinokwanisa kuita hazvigumiri pakuwana mhedzisiro.Paakatarisana nekubviswa kwechizvarwa chekutanga cheTrizol-based reagents, vanoedza vaiwanzonetseka nematanho ayo ekushanda anorema;kunyangwe mushure mekugadzirisa kwakawanda uye hunyanzvi, zvakatora nguva yakawanda.Panguva imwecheteyo, organic reagents senge phenol uye chloroform inoda kushandiswa mukuita kwekubvisa, iyo inotyisidzirawo kuchengetedzwa kwevashandi vakakodzera kusvika pamwero wakati.

Inoshanda, yakanakira uye yakachengeteka

Kutsvaga inoshanda, yakanakira uye yakachengeteka RNA yekubvisa chigadzirwa chave chinangwa chekutanga chevanoedza.Naizvozvo, Iyo Silica Spin Column adsorption nzira yeRNA yekubvisa yakatanga kuvepo.Foregene's third-generation RNA extraction products draws on the advantages of the previous generation column extraction products, using(DNA-Cleaning + RNA chete) maviri Spin Column tekinoroji kubvisa gDNA nemarara ematishu panguva imwe chete, pasina DNase kurapwa kwezvakasara gDNA, uye kusashandisa phenol, etc. Chepfu uye inokuvadza reagents, kuchena kwakaderera uye kukuvadza, kuitira kuti goho rive rakaderera uye riwane RNA.

Operation process yekuenzanisa chati

Chati yekuenzanisa yemhedzisiro yekuyedza

Foregene yakazara RNA yekuzviparadzanisa kit ine yakakwira RNA yekubvisa goho, kuchena kwakanaka, uye kubviswa kwe miRNA kuri nani pane mamwe ma brand kits.

Electrophoresis ye50 mg yembeva chiropa yakazara RNA yakatorwa nenzira dzakasiyana
Chizvarwa chekutanga (1: Trizol)
Chizvarwa chechipiri (2: RNA extraction kit kubva kukambani A)
Chizvarwa chechitatu (3: RNA extraction kit plus kubva kukambani A, 4: Foregene:RNA Isolation kits)

qPCR girafu (amplification curve graph)

Genomic DNA inofanira kunyatsobviswa panguva yeRNA kutorwa, kana zvisina kudaro ichava nemigumisiro isingadzoreki pakuedza kwakadzika (kutanga CT kukosha, nhema dzenhema).Foregene inotevera-chizvarwa kit inogona kunyatsobvisa genomic DNA pasina kuwedzera kweDNase, kuchengetedza nguva yakawanda, uku ichidzivirira kusvibiswa kunokonzerwa neiyo exogenous RNase kusvibiswa.
Chizvarwa chekutanga (girinhi inoratidza TRlzol-, tsvuku inoratidza TRlzol+)

Chizvarwa chechipiri (Blue inoratidza Kambani A RNA yekubvisa kit -, tsvuku inoratidza Kambani A RNA yekubvisa kit +)

Chizvarwa chechitatu (blue inoratidza kambani A RNA yekubvisa kit plus, green inoratidza Foregene RNA Isolation kits)

(Cherechedza: "_" mu qPCR graph inoratidza kuti DNase haina kuwedzerwa, uye kusvibiswa kweDNA hakuna kubviswa; "+" inoratidza kuti DNase yawedzerwa, uye kusvibiswa kweDNA kwabviswa)

Zadzisa RNA kudhirowa mukati me11min

Hapana chikonzero chemakore eiyo nucleic acid kudhirowa ruzivo rwekuunganidza, Foregene yakakwirira-inoshanda yakazara RNA yekuzviparadzanisa nevamwe kits akatevedzana zvigadzirwa zvinogona kukubvumidza iwe kuwana yakakwira-goho uye yakakwira-kuchena RNA mukati memaminitsi gumi nerimwe kusangana neyakasiyana-siyana yekudzika kwekuedza zvinodiwa.

Kusvika parizvino, Foregene yakazara RNA isolaton kits akateedzana zvigadzirwa zvakazivikanwa nenhamba huru yevatengi uye zvakabatsira kune akawanda epamusoro-zvibodzwa zvinyorwa.Iko kushandiswa kwemutengi kwemhinduro ye electrophoresis chati uye chiyero chePCR amplification curve mushure mekunyorwa kwereverse kunoratidza kuti RNA yakabviswa nekushandiswa kweForegene zvigadzirwa haisi yakakwirira mukusungwa, asiwo mukutendeseka kwakanaka.Chete tekinoroji yechigadzirwa iyo yakaedzwa nemusika inogona kufarirwa nemunhu wese.

Sero Yese RNA Isolation Kit

High RNA goho

Kutsanya: Pedzisa RNA Isolation mumaminitsi gumi nerimwe

Kuchengeteka: Hapana Organic kemikari yakawedzerwa

Mamwe magwaro epamusoro-soro:

1. Yuan Fang, Zezhong Liu, Yang Qiu, nevamwe.Inhibition yehutachiona suppressor yeRNAi mapuroteni nemugadziri peptides inodzivirira kubva kune enteroviral utachiona mu vivo, Immunity, 54 (10), 2021: 2231-2244.e6.(KANA:31.745,Viral RNA Isolation Kit,Katsi Nha.RE-02011)

https://www.sciencedirect.com/science/article/pii/S1074761321003617

2. Ren, Y., Wang, A., Wu, D. et al.Dual inhibition ye innate immune immune uye apoptosis nemunhu cytomegalovirus protein UL37x1 inogonesa kudzokorora kwehutachiona.Nat Microbiol 7, 1041–1053 (2022).(KANA:30.964,Sero Yese RNA Isolation Kit,Katsi Nha.RE-03111)

https://doi.org/10.1038/s41564-022-01136-6