lncRNA zvinhu:

1. lncRNAs inowanzoreba, ine simba rekutaura uye nzira dzakasiyana dzekubatanidza panguva yekusiyanisa;

2. Kuenzaniswa nekodhi yemajini, lncRNA kazhinji yakaderera;

3. Mazhinji lncRNAs ane pachena yenguva uye nzvimbo yekutaura zvakananga mukuita kwekusiyanisa kwenyama uye kukura;

4. Kune maitiro ekutaura mumamota nezvimwe zvirwere;

5. Nzvimbo dze subcellular dzelncRNA dzakasiyana;

6. Kutevedzana kwakaderera mukuchengetedza, asi basa rine imwe nhanho yekuchengetedza uye zvichingodaro.

LncRNA reverse transcription solution:

Isu tinoziva kuti lncRNA ine chimiro chepamusoro-soro mumaseru.RNA yakadaro kazhinji ine zvimiro zvakaoma senge stem loops kana hairpins.Kana iyo yechipiri chimiro chelncRNA chikatadza kugadziriswa nemazvo, reverse transcript inowanzotadza kubudirira kana kusabudirira pakuita RT-qPCR.Dambudziko rekushanda kwakaderera.

Kazhinji, pane matatu mapoinzi akakosha ekuti tiwane emhando yepamusoro cDNA matemplate:

RNA template mhando, reverse transcription primers uye reverse transcriptase, uye reverse transcriptase ndiyo yakakosha chinhu.Iyo RNA yakacheneswa neanowanzo shandiswa RNA extraction reagents kazhinji ine masara edoro uye guanidine munyu, ane simba inhibitory mhedzisiro pane akawanda reverse transcriptases;kana tembiricha yakakwana yereverse transcriptase reaction yakadzikira, haizokodzera kuRNA.Kuvhura kweiyo hierarchical structure.

Parizvino, tembiricha yakakodzera reverse transcriptase inoshandiswa mumarabhoritari mazhinji inowanzosvika 42°C, uye tembiricha yepamusoro inogona kusvika pa50°C.Muchokwadi, kuvhurwa kweiyo yepamusoro-yero RNA chimiro inoda tembiricha yepamusoro.

Iyo tembiricha yekugadzira iyo yekutanga-strand cDNA ye Fortune Biotech 2 × RT Easy TM Mix yakakwira se50 ℃-65 ℃, iyo inobatsira kuburitsa zvachose chimiro chepamusoro chelncRNA, uye nguva yekushanda ipfupi.

Pamusoro pezvo, chigadzirwa chine simba rakasimba rekurwisa-kupindira kweRNA nedoro rasara uye guanidine munyu, iyo inogona kuzadzisa zvizere zvinodikanwa zvemarabhoritari kuti iwane yakakwira reverse transcript kunyatsoita.

Yekutanga Strand cDNA Synthesis Kit

Item nhamba: RT-01011 (25 nguva)

Features:

1. Thermal kugadzikana: 65 ℃ ichine yakanaka reverse transcription chiitiko, iyo inobatsira kuvhura yakaoma yechipiri chimiro.

2. Kushivirira: cDNA synthesis inogona kubudirira kuitwa paRNA templates nekuchena kwakashata (ethanol uye guanidine salt residues)

3. Nekukurumidza: kushanda kuri nyore, yekutanga strand cDNA synthesis inogona kupedzwa mukati me25-35 maminetsi.

Zvikumbiro zvepasi: PCR, qPCR, RACE, chip labeling, kuvaka raibhurari, nezvimwe.

Pamusoro pezvo, FJ Biotech inopa yakazara seti yeRT-PCR mhinduro kuzadzisa zvinodiwa nemhando dzakasiyana dzevatengi, kusanganisira zvinotevera kits:

Yekutanga Strand cDNA Synthesis Kit (Ye qPCR)

Item nhamba: RT-01021 (50 nguva)

Features:

1. Kureruka: Reverse transcription primers dzawedzerwa kuti kushanda kuve nyore

2. Thermal kugadzikana: 65 ℃ ichine yakanaka reverse transcription chiitiko, iyo inobatsira kuvhura yakaoma yechipiri chimiro.

3. Kushivirira: cDNA synthesis inogona kubudirira kuitwa paRNA templates nekuchena kwakashata

4. Sensitivity: yakakodzera pasi-concentration RNA samples

Kudzika kwekushandisa: Kunyanya kupihwa kune inotevera qPCR

Imwe-nhanho RT-PCR kit

Nhamba yeNhamba: RT-02011 (100 times)

Features:

1. Thermal kugadzikana: 65 ℃ ichine yakanaka reverse transcription chiitiko, iyo inobatsira kuvhura yakaoma yechipiri chimiro.

2. Kushivirira: cDNA synthesis inogona kubudirira kuitwa paRNA templates nekuchena kwakashata

3. Kunyatsojeka: Iyo yakashongedzerwa PCR MIX inonyatso kunatsiridza kukwidziridza kunyatsoita uye kujeka kwemaitiro.

Kudzika kwekushandisa: cDNA cloning, genetic test uye kumwe kuedza kwema molecular

Matanho maviri RT-PCR kit

Nhamba yeNhamba: RT-02021 (100 times)

Features:

1. Nyore uye nekukurumidza: one-tube reaction

2. Thermal kugadzikana: 65 ℃ ichine yakanaka reverse transcription chiitiko, iyo inobatsira kuvhura yakaoma yechipiri chimiro.

3. Kunyatsojeka: Iyo yakawedzerwa Foregene Taq zvinobudirira inovandudza kukwidziridza kunyatsoita uye kujeka kwemaitiro.

Zvishandiso zvepasi: kuyedza kwema molecular senge genetic test

Imwe-nhanho RT-qPCR kit-SYBR Green I

Item nhamba: RT-02111 (100 times)

Features:

1. Nyore: one-chubhu reaction

2. High senitivity: inogona kuona yakaderera kopi templates

3. Kugarisana: kwakakodzera kune yakawanda fluorescence quantitative zviridzwa

Kudzika kwemvura maapplication: kucherechedzwa kwemasampunzi eRNA akadai semasero nemavhairasi

Imwe-nhanho RT-qPCR kit-Taqman

Item nhamba: RT-02131 (100 times)

Features:

1. Kurumidza: tsvaga jini rinotangwa

2. High senitivity: inogona kuona yakaderera kopi templates

3. Kugarisana: kwakakodzera kune yakawanda fluorescence quantitative zviridzwa

Kudzika kwemvura maapplication: kucherechedzwa kwemasampunzi eRNA akadai semasero nemavhairasi
Forge Bioreverse Transcription Kit inogona kukubatsira kugadzirisa zvirinani dambudziko rekureverse transcription.Panguva imwecheteyo, inobata pamwe neForge Bio RNA Extraction Kit (iyo inogona kunyatsobvisa DNA zvisaririra) kuti ikupe iwe yakakwana RNA yekuedza mhinduro.