Kunyangwe iwe uri mutsva kubhayoloji, kana mukuru mukuru ane hunyanzvi hwakasimba uye simba, wakambozvinetsa here kubvisa emhando yepamusoro yesero RNA?Vangani vanhu vanorasa masamples akakosha nekuda kwekushanda kwakaoma uye kunetsa kwechinyakare Trizol nzira?

Zvino, vhangeri riri kuuya!Nyatsoonekana kune dzinopedza nguva uye dzisingashande dzechinyakare nzira, uye nyore kubvisa yemhando yepamusoro RNA mumaminitsi gumi nematanhatu.

Sero Yese RNA Isolation Kit

Iyo sisitimu haina RNase, hapana kudiwa kwekubvisa chloroform, hakuna protein uye kumwe kusvibiswa, saka unogona kuzorora uine chokwadi.

Kunyangwe iwe uri mudiki mutsva kana mukuru mukuru, iwe unenge uine yako yakakwira zviyero zvemhando yeRNA kutorwa.

Saka rudzii rwekiti ndiyo yakakodzera kit?

Muchidimbu, zvinoreva "zvakawanda uye zvirinani."

Hapana dambudziko, gutsa kakaro kako.

Iyi kit inogona kuburitsa RNA yakazara kubva kumaseru akasimwa mu96, 24, 12, uye 6-tsime mahwendefa ane yakakwira throughput.Kune akati wandei marudzi eRNA yakadzoserwa.Pamusoro pezvakajairika 28s uye 18s zvimedu, 5s uye zvimedu zvidiki zvemiRNA zvinogona zvakare kushandiswa.Yakaunganidzwa.

Iyo yakasarudzika DNA-yekuchenesa koramu inobvumidza iwe kubvisa genomic DNA pasina kuwedzera DNase, uye mhedzisiro yacho iri nani, kuzadzisa zvaunoda kuedza zvaunoda.

Muchiona pano, munhu wese atorwa moyo here?Usanetseka, chigadzirwa chakanaka, taura nechokwadi!!!

一、Zviri nyore!(16min hakusi kungotaura)

二、Hunhu hwepamusoro!(Iyo mhedzisiro inoonekwa)

Foregene Cell Yese RNAIsolation Kits Kuenzanisa mhedzisiro ye adsorption column nzira uye Trizol nzira.

三、 Zvakanyanya kugadzikana!(Kana wangoshandisa zvinozoti zvakanaka)

Foregene QPCR patani yejini A mushure mekudzoserwa kunyorwa

Mazano:

Kana huwandu hwemasero hwakanyanya kuderera, iyo yakazara RNA goho ichaderedzwa, uye spectrophotometer haigone kunyatsoona kukosha kweOD.Zvinokurudzirwa kuita bvunzo dzemhando kuburikidza neanotevera qPCR bvunzo.Ichi chigadzirwa chinoshanda zvirinani nekambani yedu RT EasyTM II (Yekutanga-strand cDNA synthesis yeChaiyo Nguva PCR) (RT-01022)!

Sarudza Foregene Yakakura Sero Yese RNA Yekuwedzera Kit kuti kuyedza kwako nhanho imwe nekukurumidza.Wakamirirei, ita izvozvi!