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Rakabudiswa ne: CAS Key Laboratory yePathogen Microbiology uye Immunology, Institute of Microbiology, Chinese Academy of Sciences

IF: 69.504

Assisted Product:Sero Yese RNA Isolation Kit

Original Link:https://www.nature.com/articles/s41586-022-05271-2

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Utachiona hune filoviruses hwakadai seEbola virus (EBOV) uye hutachiona hweMarburg hunogona kukonzera zviratidzo zvakakomba zvekiriniki, kusanganisira hemorrhagic fever uye kutadza kwenhengo dzakawanda.4.Pano takasarudza chimiro cheEbola virus L protein yakaoma ine tetrameric VP35 uchishandisa cryo-electron microscopy (nyika 1).Ongororo yemaitiro yakaratidza kuti hutachiona hweEbola L ine filovirus-chaiyo yekuisa chinhu chakakosha paRNA synthesis, uye kuti VP35 inodyidzana zvakanyanya neN-terminal dunhu reL nemaprotomer matatu eVP35 tetramer.

Mupepa iri, RNA yakazara yakatorwa pachishandiswa ForegeneSero Yese RNA Isolation Kituye kuwanda kweRNA kwakayerwa neNanodrop pa260 nm, uyezve kuita RT/qPCR yekuona.

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∎ Iyo yese nzira inoshandiswa pakudziya kwekamuri (15-25 ℃), pasina kugeza echando uye yakaderera tembiricha centrifugation.

■ Kiti yese ndeyeRNase-Yemahara, hapana chikonzero chekunetseka nezveRNA kuparara.

■ DNA-Cleaning Column inosunga zvakananga DNA, kuitira kuti kit ikwanise kubvisa genomic DNA kusvibiswa pasina kuwedzera imwe DNase.

■ Yakakwira RNA goho: RNA-chete Column uye yakasarudzika fomula inogona kunyatsochenesa RNA.

■ Kukurumidza kumhanya: nyore kushanda uye inogona kupedzwa mumaminitsi gumi nerimwe.

■ Chengetedzo: Hapana organic reagent inodiwa.

■ Hunhu hwepamusoro: RNA yakacheneswa ndeyekuchena kwepamusoro, haina mapuroteni uye kumwe kusvibiswa, uye inogona kusangana nemiedzo yakasiyana-siyana inotevera.


Nguva yekutumira: Nov-04-2022