Rakabudiswa ne: CAS Key Laboratory yePathogen Microbiology uye Immunology, Institute of Microbiology, Chinese Academy of Sciences
IF: 69.504
Assisted Product:Sero Yese RNA Isolation Kit
Original Link:https://www.nature.com/articles/s41586-022-05271-2
Utachiona hune filoviruses hwakadai seEbola virus (EBOV) neMarburg virus hunogona kukonzera zviratidzo zvakakomba zvekiriniki, kusanganisira hemorrhagic fever uye multiorgan kutadza.4.Pano takasarudza chimiro cheEbola virus L protein yakaoma ine tetrameric VP35 uchishandisa cryo-electron microscopy (nyika 1).Ongororo yemaitiro yakaratidza kuti Ebola virus L ine filovirus-specific insertion element yakakosha paRNA synthesis, uye kuti VP35 inodyidzana zvakanyanya neN-terminal dunhu reL nemaprotomer matatu eVP35 tetramer.
Mupepa iri, RNA yakazara yakatorwa pachishandiswa ForegeneSero Yese RNA Isolation Kituye kuwanda kweRNA kwakayerwa neNanodrop pa260 nm, uyezve kuita RT/qPCR yekuona.
∎ Iyo yese nzira inoshandiswa pakudziya kwekamuri (15-25 ℃), pasina kugeza echando uye yakaderera tembiricha centrifugation.
■ Kiti yese ndeyeRNase-Yemahara, hapana chikonzero chekunetseka nezveRNA kuparara.
■ DNA-Cleaning Column inosunga zvakananga DNA, kuitira kuti kit ikwanise kubvisa genomic DNA kusvibiswa pasina kuwedzera imwe DNase.
■ Yakakwira RNA goho: RNA-chete Column uye yakasarudzika fomula inogona kunyatsochenesa RNA.
■ Kukurumidza kumhanya: nyore kushanda uye inogona kupedzwa mumaminitsi gumi nerimwe.
■ Chengetedzo: Hapana organic reagent inodiwa.
■ Hunhu hwepamusoro: RNA yakacheneswa ndeyekuchena kwepamusoro, haina mapuroteni uye kumwe kusvibiswa, uye inogona kusangana nemiedzo yakasiyana-siyana inotevera.
Nguva yekutumira: Nov-04-2022
