Inopfuura hafu ye2022, huya uone izvo zvinyorwa zvepamusoro-soro zvakatumirwa naFoeregene!
01
IF:17.694
Rakabudiswa ne: The First Affiliated Hospital,Sun Yat-sen University
Zvikuru zvemukati: Esophageal squamous cell carcinoma (ESCC) ndiyo huru subtype yegomarara resophageal.Kuziva zvinobudirira zvekurapa zvinangwa zvakakosha zvikuru kurapwa kurinani kweESCC.Zvimwe zvidzidzo zvakaratidza kuti RNA mismodification inogona kusimudzira kutaura uye dudziro yekenza cell-inosanganisirwa mRNAs, asi mamorekuru maitiro anoramba asina kujeka.Ichi chidzidzo chakaratidza kuti tRNA m7G methyltransferase-yakabatana mapuroteni akaoma METTL1 uye WDR4 akawedzera zvakanyanya muESCC tishu uye akabatsira mukusaziva kwe ESCC.Kubva ipapo, METTL1 uye WDR4-inoenderana neiyo gene knockout zvidzidzo muESCC modhi mbeva zvakasimbisa kuti iyo inosimudzira chiitiko che tRNA m7G methyltransferase inotungamira kune kumusoro-mutemo wekutaura kwemapuroteni maviri aya, izvo zvinosimudzira kuwanda kwemasero eESCC.Ichi chidzidzo chinoratidza zvakakosha oncogenic basa rekusagadziriswa tRNA m7G kugadziriswa muESCC uye inoratidza kuti kunanga METTL1 uye nzira dzayo dzezasi dzechiratidzo dzinogona kunge dziri kuvimbisa kurapa kwekurapa kweESCC therapy.
Batsira chigadzirwa:
Mouse Muswe Wakananga PCR Kit Forgene#TP-01333
Original link:
https://www.nature.com/articles/s41467-022-29125-7
02
IF:14.903
Rakadhindwa ne: State Key Laboratory yeQuality Research muChinese Medicine, Institute of Chinese Medical Sayenzi, University of Macau
Main content:
Nekuvandudzwa kwehunyanzvi hwekugadzira, mRNA yave kirasi nyowani yemishonga yekurapa.Nekudaro, kuwana inoshanda intracellular kuendesa kwemRNA mishonga inoramba ichinetsa nekuda kwekunzwa kwemRNA kune RNases.Ichi chidzidzo chinopa yakanakira kuburitsa chirongwa chepolyphenol-conjugated nanostructures inogona kunyatso buritsa IL-10 mRNA in vivo.Mushure mekushandisa iyi nanostructure kuendesa mRNA mishonga, IL-10 mazinga akanga akanyatsogadziriswa, achinyatsodzivisa kutaura kwezvinhu zvinoputika, kukurudzira kugadziriswa kwemucosal, kuchengetedza colonic epithelial masero kubva kuapoptosis, uye inhibiting dextran sodium sulfate-induced acute uye isingagumi yechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chechirwere chinopisa.
Batsira chigadzirwa:
Mhuka Yese RNA Isolation Kit Foregene#RE-03011
Original link:
03
IF:12.499
Rakabudiswa ne: Laboratory yeExperimental Animal Disease Model, Koreji yeVeterinary Medicine, Sichuan Agricultural University
Main content:
Oxidative stress yesperm ndicho chinhu chikuru chinotungamira kukushaya mbereko kwemurume, saka isarudzo inogoneka uye inoshanda yekumisikidza mhando dzemhuka dzechirume dzekushaya mbereko nekugadzirisa iyo antioxidant system nenzira yakanangwa.Nekudaro, echirume germ cell-specific antioxidant nzira hadzina kujekeswa.Chidzidzo ichi chakasimbisa kuti LanCL1 gene iine antioxidant gene mumasero echirume echirume, uye kutaura kwayo kunodzikiswa kana kubatanidzwa nekushaya mbereko kwevanhu.Mbeva dzechirume dzakashomeka mujene reLanCL1 dzinoratidza kukuvadzwa kwembeu yeoxidative uye kukanganisa kubereka.Mukati mekuita kwekusiyaniswa kwembeu yemasero ejemu, kana oxidation tendency yaitika, transcription factor-specific protein 1 (SP1) inotonga kutaura kweLanCL1 gene, nokudaro ichivharira maitiro eoxidation yembeu.Ongororo iyi inoratidza kuti nzira yeSP1-LanCL1 inoita basa rakakosha mukudzora testicular homeostasis uye kuzvara kwechirume kunoyananiswa neredox balance, uye inoratidza kuti mbeva dzakagadziridzwa LanCL1 dzine maapplications anoyevedza semuenzaniso wemhuka wekushaya mbereko kwemurume.
Assisted Product:
Mouse Muswe DNA Mini Kit Forgene#DE-05211
Original Link:
http://europepmc.org/article/MED/35469022
◮Hapana chinopedza nguva uye chinodhura chekuchenesa DNA chinodiwa.
◮Sample inodiwa idiki, ingotora mhodzi imwe chete.
◮Hapana kurapwa kwakakosha senge kukuya nekupwanya kunodiwa, uye kuvhiya kuri nyore.
◮Iyo yakagadziridzwa PCR sisitimu inoita kuti PCR ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.
◮Hapana chikonzero chekunetseka nezveRNA kudzikira.Iyo yese system ndeye RNase-Yemahara.
◮Kubvisa DNA zvine mutsindo uchishandisa DNA-Kuchenesa Column.
◮Bvisa DNA pasina kuwedzera DNase.
◮Zviri nyore—mabasa ose anopedzwa patembiricha yekamuri.
◮Kukurumidza—kushanda kunogona kupedzwa mumaminitsi makumi matatu.
◮Yakachengeteka—hapana organic reagent inoshandiswa.
◮Kuchena kwepamusoro—OD260/280≈1.8-2.1.
◮Hapana RNase kusvibiswa:DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva mujenomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.
◮KumhanyaForegene Protease ine chiitiko chepamusoro kupfuura maprotease akafanana, uye inogaya masampula enyama nekukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me20-80 maminetsi.
◮Yakanakira:Iyo centrifugation inoitwa pakamuri tembiricha, uye hapana chikonzero che4 ° C yakaderera-tembiricha centrifugation kana ethanol kunaya kweDNA.
◮Safety:Hapana organic reagent kutorwa kunodiwa.
◮Hunhu hwepamusoro:Iyo yakabviswa genomic DNA ine zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
◮Micro-elution system:Inogona kuwedzera kuwanda kweiyo genomic DNA, iyo yakanakira kudzika kwerwizi kana kuyedza.
Nguva yekutumira: Sep-13-2022
