Background
Mumakore achangopfuura, extracellular vesicles (EVs) akakwezva pfungwa dzevanhu sechinhu chinogona kurapa chishandiso;zvisinei, kurapa kweEVs pa endometriosis hakuna kutaurwa.Endometriosis chirwere chisiri chakashata chegynecological chinobata 10-15% yevakadzi vezera rekubereka, zvichikonzera zviratidzo zvakasiyana, zvichikonzera kudzikira kwehupenyu uye mutoro mukuru wemagariro.
Chinyorwa Nhanganyaya
Musi waChikunguru 20, 2021, boka rekutsvagisa raProfessor Wang Guoyun kubva kuQilu Hospital yeShandong University rakaburitsa bepa rekutsvagisa rakanzi "M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis" paFrontiers in immunology, iyo yakakurukura nezve1 macrophages yeM2.Iko kugona kwe nanovesicles (NVs) mukurapa kwe endometriosis.
Ichi chinyorwa chinoshandisa inoenderera mberi extrusion nzira kugadzirira M1NVs, uye inoshandisa co-tsika nzira yekudzidza shanduko yeangiogenesis, kutama, kupinda uye zvimwe zviratidzo zveeutopic endometrial stromal masero (EM-ESCs) kubva kuvarwere vane endometriosis.Panguva imwecheteyo, modhi yembeva ye endometriosis yakatangwa, uye mbeva dzakarapwa nePBS, MONVs kana M1NVs, zvichiteerana, kuongorora kushanda nekuchengetedzeka kweM1NV mukurapa endometriosis.
Mhedzisiro yacho yakaratidza kuti in vitro M1NVs inogona kumisa zvakananga kana zvisina kunanga kutama uye kupinda kweEM-ESCs, uye inhibit angiogenesis.Mumuenzaniso wembeva, M1NVs inodzivisa kuitika kweendometriosis kuburikidza neM2 macrophage reprogramming pasina kukonzera kukuvara kwenhengo.Zvinotaridza kuti M1NVs inogona kuvharisa zvakananga kuitika kweendometriosis, uye zvakare inogona kuvharidzirwa nekudzokorora M2 mhando macrophages kune M1 mhando.Naizvozvo, kushandiswa kweM1NVs inogona kunge iri nzira nyowani yekurapa endometriosis.
Foregene Help
Muchidzidzo, nokuti M1NV yakagadzirirwa nekuramba ichisvina M1 macrophages, chinyorwa chakashandisa qRT-PCR kuona zvinhu zvinopisa uye M1 macrophage markers iNOS, TNF-a uye IL-6 mRNA muM1NV uye M1 macrophages.Relative multiples of change.Mhedzisiro yacho yakaratidza kuti M1NVs yaive nepro-inflammatory factor mRNA uye M1 macrophage mamaki, zvichiratidza kuti M1NVs inogona kuchengetedza zvinobudirira maitiro eM1 masero.Iyi nzira yekutsvagisa inoshandisa iyo QuickEasy Cell Direct RT-qPCR Kit-SYBR Green I yeForegene.
Sero Yakananga RT-qPCR Kitdetails

Mamiriro ekushandisa:
 
1. Gene regulation uye ongororo yekutaura, verification ye gene overexpression kana kukanganisa maitiro, kuongorora zvinodhaka, nezvimwewo;
2. Kuonekwa kwemajini ekutaura kwemasero akaoma-kurimwa akadai semaprimary cells, stem cells, uye nerve cells;
3. Kuonekwa kwemRNA mumasampuli akadai se exosomes uye nanovesicles.
Features: