W: Hama, iyo reverse transcription yapera, uye pakupedzisira danho rekupedzisira - fluorescent quantitative PCR!Nhai, hama, chii chinonzi real-time quantitative PCR?
M: PCR unogara uchiziva, handiti?
W: Unoziva, PCR ndiyo polymerase chain reaction, uye inyanzvi inowedzera DNA!
M:Zvinenge…Iri nzira yehunyanzvi yekuwedzera kutevedzana kwechinangwa kusvika kanopfuura miriyoni imwe kuburikidza nemaprimers chaiwo, asi izvi zvinongoratidza kuvepo kana kusavapo kwekutevedzana kwechinangwa, uye hazvigone kuona huwandu hwekutanga template.Fluorescence quantitative PCR inzira yehunyanzvi yakagadziridzwa kugadzirisa dambudziko iri.Real time PCR uye uwandu PCR (qPCR) kazhinji inoreva nezvayo.
W: Musiyano kubva kuPCR wakajairwa uri mufluorescence quantification?
M: Ehe, ndiko kuti, muchikamu chekuwedzera chePCR kutenderera kwega kwega, iyo fluorescence chiratidzo chesimba inoonekwa, uye yekutanga template yemari inogona kuverengerwa zvichienderana nekushandurwa kwechiratidzo chesimba.
W: Zvinonzwa zvakaoma!Ndinofanira kuverenga izvi ndega here?
M: Izvi zvinoda kuti udzidze zvishoma nezvishoma, zviri nani kuti ugone kunzwisisa musimboti.Iye zvino iwe unogona kutarisa pane CT kukosha kwekutanga, inova intuitive yekuedza data, uye ukuru hwayo hunoenderana nehukuru hwekutanga template huwandu.Kune zvakare magirafu akadai seamplification curves uye kunyunguduka macurves.Ivo vane rekupedzisira rekuti kuedza kwako kwakabudirira here kana kuti kwete.
W: Ah, mukoma.Ipapo ini ndoita qPCR kuyedza!
M: Chengetedza zvakanyanya kuti usawedzera matemplate kunzvimbo ye reagent, zvikasadaro musanganiswa wako usina kushandiswa uchasvibiswa nyore!
W: Ndazvinzwa!

Real Time PCR Easyᵀᴹ-SYBR Green I Kit

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