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Buccal swab / FTA kadhi DNA yekubvisa kit

Kuongorora kunotevera kwematambudziko anogona kuitika muBuccalswab/FTA card DNA extraction is helpful to your experiment. In addition, for other experimental or technical problems in addition to operating instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us at: 028-83360257 or E-mali:Tech@foregene.com.

 

Koramu yekuchenesa yakavharwa

 

 

Mune kit iyi, mune genomic DNA yekubvisa oparesheni, mbiru yekuchenesa inoshambadzirwa zvakananga pane sampuli enzymatic lysis musanganiswa pasina centrifugation nhanho, uye mbiru yekuchenesa inogona kuvharika nekuda kwekusakwana enzymization uye yakakwirira viscosity yemuenzaniso.

Zvikonzero zvinobvira zvinotevera zvinotevera:

1. Kusakwana enzymatic digestion yemasampuli ematishu.

Kurudziro: Iyo yekuenzanisira yekugadzirisa nguva yeForegene Protease inogona kuwedzerwa zvakafanira kana iyo supernatant inogona kutorwa mushure me centrifugation pa12,000 rpm (~ 13,400× g) kwe5 min.

2. Kunyanya kushandiswa kwematishu samples kana matishu makuru.

Kurudziro: Zvakanakisa kuti usapfuure 1Buccal swab mumuenzaniso;kana sampuli yakakura kwazvo, wedzera dosi yeBuffer ST1, Foregene Protease, buffer ST2 maererano.

3. Muenzaniso viscosity yakanyanyisa.

Kurudziro: Masamples anogona kucheneswa zvakafanira ne10 mM yeTris-HCl isati yatorwa genomic DNA.

4. Zvimedu zvekadhi reropa zvakayamwa.

Kurudziro: Iyo inopfupika centrifugation nguva yenhanho 6 yeropa nzvimbo (FTA Kadhi) genomic extraction inogona kuwedzerwa zvakafanira.

 

Goho rakaderera kana pasina DNA

 

Pane kazhinji zvinhu zvakasiyana-siyana zvinokanganisa goho reGenomic DNA, kusanganisira mabviro emuenzaniso, mamiriro ekuchengetedza emuenzaniso, kugadzirira kwemuenzaniso, kunyengedza, nezvimwe.

Genomic DNA haigone kuwanikwa panguva yekubvisa

Zvikonzero zvinobvira ndezvizvi:

1. Kuchengetedzwa kusina kunaka kwemasampuli kana kuchengetwa kwenguva yakareba kunotungamirira kune genomic DNA degradation.

Kurudziro: Oral swabs inofanirwa kuve ichangobva sampuli, uye hazvikurudzirwe kushandisa yakachengetedzwa swabs ye genomic DNA yekubvisa mashandiro;masampu eropa anofanirwa kuve nechokwadi chekuti mhando yacho inokodzera uye nguva yekuchengetedza haifanirwe kunge yakareba.

2. Kunyanya kushandiswa kwetishu kunogona kukonzera kusatorwa kweiyo genomic DNA inoenderana.

Kurudziro: Tevedzera iyobuccal swab sampling mirayiridzo mugwaro rekushanda, uye pukuta kakawanda sezvinobvira kuitira kuti masero akakwana agone kusungirirwa kune oral swab ye genomic DNA kutorwa;pakutorwa kwesampuli yeropa, nzvimbo yekucheka ropa inogona kuwedzerwa zvakakodzera.

3. Foregene Protease yakachengetedzwa zvisina kunaka, zvichiita kuti iderere mukuita kwayo kana kusashanda.

Kurudziro: Simbisa mamiriro ekuchengetedza eiye Foregene Protease kana kuitsiva neForegene Protease itsva ye enzymatic reaction.

4. Kuchengetedza kusina kukodzera kwekiti kana nguva yekuchengetedza yakanyanyisa, zvichiita kuti kukundikana kwezvimwe zvikamu mukiti.

Kurudziro: Tenga itsvaBuccal swab DNAkuiswa kwemurwere kwake ega kit yemaitiro anoenderana.

5. Buffer WB haiwedzeri absolute ethanol.

Kurudziro: Simbisa kuti buffer WB inowedzera huwandu chaihwo hweabsolute ethanol.

6. Iyo yakashata haina kunyatsowedzerwa kune silicone film.

Kurudziro: Wedzera 65°Cpre-kudziirwa eluent inodonha kusvika pakati pesilicone membrane uye wosiya pakamuri tembiricha kwemaminetsi mashanu kuti uwedzere kugona.

LOw-yield genomic DNA isolated

Zvikonzero zvinobvira zvinotevera zvinotevera:

1. Kuchengetedzwa kusina kunaka kwemasampuli kana kuchengetwa kwenguva yakareba kunotungamirira kune genomic DNA degradation.

Kurudziro: Oral swabs anonyanya kutorwa samples, uye akachengetedzwa swabs haafanirwe kushandiswa kune genomic DNA kutorwa.

2. Kana chiyero chetishu sampuli chiri chidiki, iyo yakabviswa genomic DNA zvinyorwa zvichave zvishoma.

Kurudziro: Tevedzera iyo oral swab sampling rairo mugwaro rekushandisa, uchipukuta kakawanda sezvinobvira kuitira kuti masero akakwana agone kusungirirwa pamuromo swab ye genomic DNA kutorwa.

3. Foregene Protease yakachengetedzwa zvisina kunaka, zvichiita kuti iderere mukuita kwayo kana kusashanda.

Kurudziro: Simbisa mamiriro ekuchengetedza ethe Foregene Protease kana kuitsiva neimwe itsva Foregene Protease ye enzymatic reaction.

4. Eluent matambudziko.

Kurudziro: Shandisa Buffer EB ye elution;kana uchishandisa ddH2O kana zvimwewo zvinonzwisisika, simbisa kuti pH yeiyo eluate iri pakati pe7.0-8.5.

5. The eluate haina kunyatsowedzerwa dropwise.

Kurudziro: Wedzera madonhwe emvura pakati pesilicone membrane uye siya pakamuri tembiricha kwemaminetsi mashanu kuti uwedzere kushanda kwe elution.

6. The elution liquid inoungana zvishoma.

Kurudziro: Shandisa eluent ye genomic DNA elution sezvinodiwa mumirayiridzo, zvirinanikwete zvishoma kupfuura 15μl.

Low kuchena of genomic DNAisolated

Low genomic DNA kuchena kunogona kutungamirira mukukundikana kana kusagutsikana kwemigumisiro yezviyedzo zvepasi, zvakadai se: ma enzymes haagone kuchekwa akazaruka, PCR haigoni kuwana gene fragment yekufarira, nezvimwewo.

Zvikonzero zvinobvira ndezvizvi:

1. Heteroprotein kusvibiswa, RNA kusvibiswa.

Kuongorora: Koramu yekuchenesa haina kugezwa uchishandisa Buffer PW;iyo Buffer PW yekuchenesa koramu haina kugezwa uchishandisa iyo chaiyo centrifugal kumhanya.

Kurudziro: Iva nechokwadi chekuti hapana kunaya musimba repamusoro usati wawedzera ethanol;iva nechokwadi chekushambidza mbiru yekuchenesa maererano nemirairo, uye danho iri harigoni kusiyiwa.

2. Kusvibiswa ion kusvibiswa.

Ongororo: Buffer WB yekugezesa koramu yakasiiwa kana kuwachwa kamwe chete, zvichikonzera kusvibiswa kweionic kwasara.

Kurudziro: Iva nechokwadi chekugeza Buffer WB kaviri sekurairwa kuti ubvise maion akasara zvakanyanya sezvinobvira.

3. RNA enzyme kusvibiswa.

Analysis: Foreign RNases dzakawedzerwa kune buffer;Buffer PW wash operation yanga isiriyo, zvichikonzera RNase zvisaririra, zvichikanganisa maitirwo ekuedza eRNA ezasi, senge in vitro transcription.

Kurudziro: Foregene series nucleic acidisolation kits inogona kubvisa RNA pasina kuwedzera kweRNase,saizvozvo buccal Swab/FTA Kadhi DNA Isolation Kitneedn't wedzera RNase;iva nechokwadi chekutevera mirairo yeBuffer PW yekuchenesa mbiru, uye danho iri harigone kusiiwa.

4. Ethanol yakasara.

Ongororo: Buffer WB haina kuita isina chinhu chubhu centrifugation mushure mekugeza koramu yekuchenesa.

Kurudziro: Ita iyo chaiyo isina chinhu chubhu centrifugation oparesheni zvinoenderana nemirairo.

5. Kumwe kusvibiswa kwetsvina.

Ongororo: Samples dzakachengetwa kana akakosha masampuli haana preteated.

Kurudziro: Nyatsofanogadzirira muenzaniso sezvaunenge warairwa.

 


Nguva yekutumira: Mar-18-2022